摘要
目的:探讨三七总皂苷(PNS)肠溶微丸对家兔血液流变学的影响。方法:建立空白对照组、模型组、血栓通注射剂(冻干)组(15 mg·kg~(-1)·d~(-1),im)、PNS肠溶微丸高剂量组(45 mg·kg~(-1)·d~(-1),ig)、中剂量组(30 mg·kg~(-1)·d~(-1),ig)、低剂量组(15 mg·kg~(-1)·d~(-1),ig),予高脂饲料配方灌胃造模;运用血液流变学检测方法测定各组全血黏度、血浆黏度、红细胞聚集指数、红细胞刚性指数、红细胞电泳率5项指标。结果:模型组血液流变学5项指标明显高于空白对照组(P<0.01),提示动物模型复制成功。与模型组比较,PNS肠溶微丸高、中剂量组均能明显降低全血黏度和血浆黏度(P<0.01或P<0.05);PNS肠溶微丸低剂量组能显著降低中切黏度及血浆黏度(P<0.05);PNS肠溶微丸高、中、低剂量组红细胞聚集指数显著降低(P<0.01);PNS肠溶微丸高、中剂量组红细胞刚性指数显著降低(P<0.01或P<0.05);PNS肠溶微丸高、中、低剂量有降低红细胞电泳率的趋势,但差异无统计学意义(P>0.05)。PNS肠溶微丸中剂量组降低中切黏度效果优于血栓通注射剂(冻干)组(P<0.05)。结论:PNS肠溶微丸能降低家兔全血黏度、血浆黏度、红细胞聚集指数、红细胞刚性指数、红细胞电泳率等指标,发挥PNS肠溶微丸活血化瘀、抑制血栓形成、增加心脑血管的血液供应的作用。
Objective: To discuss the effects of panax notoginseng saponins( PNS) enteric-coated pellets on hemorrheology in rabbits. Methods: The rabbits were divided into the normal control group,the model control group,Xueshuangtong injection( lyophilization) group(15 mg·kg~(-1)·d~(-1),im),PNS enteric-coated pellets groups respectively at high(45 mg·kg~(-1)·d~(-1),ig),medium(30mg·kg~(-1)·d~(-1),ig) and low(15 mg·kg~(-1)·d~(-1),ig) dose. The model was established by intragastric administration of high-fat diet. The whole-blood viscosity,plasma viscosity,erythrocyte aggregational index,crythrocyte index of rigidity and erythrocyte electrophoresis rate in the groups were detected using hemorheological methods. Results: The above indices of hemorheology in the model control group were all significantly higher than those in the normal control group( P < 0. 01),suggesting the successful establishment of the model. Compared with the model control group,PNS enteric-coated pellets at high and medium doses could significantly reduce the whole blood viscosity and plasma viscosity( P < 0. 01 or P < 0. 05); PNS enteric-coated pellets group at low dose could significantly reduce the whole blood middle shear viscosity and plasma viscosity( P < 0. 05); the three PNS enteric-coated pellets groups could significantly reduce the agglutination index( P < 0. 01); PNS enteric-coated pellets at high and medium doses could significantly reduce the crythrocyte index of rigidity( P < 0. 01 or P < 0. 05); the three PNS enteric-coated pellets groups could significantly reduce the erythrocyte electro-phoresis rate,while there was no significant difference( P > 0. 05). Compared with Xueshuangtong injection( lyophilization) group,PNS enteric-coated pellets group at medium dose could significantly reduce the whole blood middle shear viscosity( P <0. 05). Conclusion: PNS enteric-coated pellets can reduce the whole-blood viscosity,plasma viscosity,erythrocyte aggregational index,crythrocyte index of rigidity and erythrocyte electro-phoresis rate,and effectively promote blood circulation and remove stasis,inhibit thrombosis formation and increase blood supply for heart and cerebral vessels.
引文
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