三七总皂苷肠溶微丸对高脂血症家兔血液流变学的影响
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摘要
目的:探讨三七总皂苷(PNS)肠溶微丸对家兔血液流变学的影响。方法:建立空白对照组、模型组、血栓通注射剂(冻干)组(15 mg·kg~(-1)·d~(-1),im)、PNS肠溶微丸高剂量组(45 mg·kg~(-1)·d~(-1),ig)、中剂量组(30 mg·kg~(-1)·d~(-1),ig)、低剂量组(15 mg·kg~(-1)·d~(-1),ig),予高脂饲料配方灌胃造模;运用血液流变学检测方法测定各组全血黏度、血浆黏度、红细胞聚集指数、红细胞刚性指数、红细胞电泳率5项指标。结果:模型组血液流变学5项指标明显高于空白对照组(P<0.01),提示动物模型复制成功。与模型组比较,PNS肠溶微丸高、中剂量组均能明显降低全血黏度和血浆黏度(P<0.01或P<0.05);PNS肠溶微丸低剂量组能显著降低中切黏度及血浆黏度(P<0.05);PNS肠溶微丸高、中、低剂量组红细胞聚集指数显著降低(P<0.01);PNS肠溶微丸高、中剂量组红细胞刚性指数显著降低(P<0.01或P<0.05);PNS肠溶微丸高、中、低剂量有降低红细胞电泳率的趋势,但差异无统计学意义(P>0.05)。PNS肠溶微丸中剂量组降低中切黏度效果优于血栓通注射剂(冻干)组(P<0.05)。结论:PNS肠溶微丸能降低家兔全血黏度、血浆黏度、红细胞聚集指数、红细胞刚性指数、红细胞电泳率等指标,发挥PNS肠溶微丸活血化瘀、抑制血栓形成、增加心脑血管的血液供应的作用。
Objective: To discuss the effects of panax notoginseng saponins( PNS) enteric-coated pellets on hemorrheology in rabbits. Methods: The rabbits were divided into the normal control group,the model control group,Xueshuangtong injection( lyophilization) group(15 mg·kg~(-1)·d~(-1),im),PNS enteric-coated pellets groups respectively at high(45 mg·kg~(-1)·d~(-1),ig),medium(30mg·kg~(-1)·d~(-1),ig) and low(15 mg·kg~(-1)·d~(-1),ig) dose. The model was established by intragastric administration of high-fat diet. The whole-blood viscosity,plasma viscosity,erythrocyte aggregational index,crythrocyte index of rigidity and erythrocyte electrophoresis rate in the groups were detected using hemorheological methods. Results: The above indices of hemorheology in the model control group were all significantly higher than those in the normal control group( P < 0. 01),suggesting the successful establishment of the model. Compared with the model control group,PNS enteric-coated pellets at high and medium doses could significantly reduce the whole blood viscosity and plasma viscosity( P < 0. 01 or P < 0. 05); PNS enteric-coated pellets group at low dose could significantly reduce the whole blood middle shear viscosity and plasma viscosity( P < 0. 05); the three PNS enteric-coated pellets groups could significantly reduce the agglutination index( P < 0. 01); PNS enteric-coated pellets at high and medium doses could significantly reduce the crythrocyte index of rigidity( P < 0. 01 or P < 0. 05); the three PNS enteric-coated pellets groups could significantly reduce the erythrocyte electro-phoresis rate,while there was no significant difference( P > 0. 05). Compared with Xueshuangtong injection( lyophilization) group,PNS enteric-coated pellets group at medium dose could significantly reduce the whole blood middle shear viscosity( P <0. 05). Conclusion: PNS enteric-coated pellets can reduce the whole-blood viscosity,plasma viscosity,erythrocyte aggregational index,crythrocyte index of rigidity and erythrocyte electro-phoresis rate,and effectively promote blood circulation and remove stasis,inhibit thrombosis formation and increase blood supply for heart and cerebral vessels.
Objective: To discuss the effects of panax notoginseng saponins( PNS) enteric-coated pellets on hemorrheology in rabbits. Methods: The rabbits were divided into the normal control group,the model control group,Xueshuangtong injection( lyophilization) group(15 mg·kg~(-1)·d~(-1),im),PNS enteric-coated pellets groups respectively at high(45 mg·kg~(-1)·d~(-1),ig),medium(30mg·kg~(-1)·d~(-1),ig) and low(15 mg·kg~(-1)·d~(-1),ig) dose. The model was established by intragastric administration of high-fat diet. The whole-blood viscosity,plasma viscosity,erythrocyte aggregational index,crythrocyte index of rigidity and erythrocyte electrophoresis rate in the groups were detected using hemorheological methods. Results: The above indices of hemorheology in the model control group were all significantly higher than those in the normal control group( P < 0. 01),suggesting the successful establishment of the model. Compared with the model control group,PNS enteric-coated pellets at high and medium doses could significantly reduce the whole blood viscosity and plasma viscosity( P < 0. 01 or P < 0. 05); PNS enteric-coated pellets group at low dose could significantly reduce the whole blood middle shear viscosity and plasma viscosity( P < 0. 05); the three PNS enteric-coated pellets groups could significantly reduce the agglutination index( P < 0. 01); PNS enteric-coated pellets at high and medium doses could significantly reduce the crythrocyte index of rigidity( P < 0. 01 or P < 0. 05); the three PNS enteric-coated pellets groups could significantly reduce the erythrocyte electro-phoresis rate,while there was no significant difference( P > 0. 05). Compared with Xueshuangtong injection( lyophilization) group,PNS enteric-coated pellets group at medium dose could significantly reduce the whole blood middle shear viscosity( P <0. 05). Conclusion: PNS enteric-coated pellets can reduce the whole-blood viscosity,plasma viscosity,erythrocyte aggregational index,crythrocyte index of rigidity and erythrocyte electro-phoresis rate,and effectively promote blood circulation and remove stasis,inhibit thrombosis formation and increase blood supply for heart and cerebral vessels.
引文
1赖玲,刘华钢,文丽,等.空白丸芯上药法制备三七总皂苷肠溶微丸[J].广西医科大学学报,2011,28(5):681
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7 陈素红,吕圭源,高建莉,等.复方决明对大鼠血液流变学的影响[J].中药药理与临床,2003,19(6):40-41
8 马珂,汤金土.三七皂甙的实验研究进展[J].浙江中西医结合杂志,2002,12(3):197-199
9 陈汉广,王良盟,刘新.麝香保心丸联合常规治疗对不稳定型心绞痛患者的疗效及血液流变学的影响[J].中国药师,2014,17(7):1166-1168
10 马伯艳,袁良杰,牛雯颖,等.延胡索乙素对老龄大鼠血液黏度及红细胞变形性的影响[J].中医药信息,2011,28(5):100-101
2 赖玲.三七总皂苷肠溶制剂的研究[D].南宁:广西医科大学博士学位论文,2010
3 秦艳娥,刘华钢,陆仕华,等.三七总皂苷肠溶微丸和三七总皂苷对家兔动脉粥样硬化预防作用比较[J].中国实验方剂学杂志,2013,19(12):261-264
4 许淑清,赵学伟,陈明.三七及其提取物在制备治疗和/或预防PTCA术后再狭窄药物的用途的制作方法[P].中国专利:CN200910223951,2010-4-28
5 曾庆锋,谭军.枸骨叶提取物对高脂血症模型大鼠脂代谢及血液流变学的影响[J].中国药师,2016,19(4):631-638
6 吴巧凤,郝云云,严云良.祛瘀清热颗粒对血瘀模型家兔血液流变学的影响[J].中华中医药学刊,2014,32(3):458-459
7 陈素红,吕圭源,高建莉,等.复方决明对大鼠血液流变学的影响[J].中药药理与临床,2003,19(6):40-41
8 马珂,汤金土.三七皂甙的实验研究进展[J].浙江中西医结合杂志,2002,12(3):197-199
9 陈汉广,王良盟,刘新.麝香保心丸联合常规治疗对不稳定型心绞痛患者的疗效及血液流变学的影响[J].中国药师,2014,17(7):1166-1168
10 马伯艳,袁良杰,牛雯颖,等.延胡索乙素对老龄大鼠血液黏度及红细胞变形性的影响[J].中医药信息,2011,28(5):100-101