基于形态学性状和SSR标记的花生品种遗传多样性分析和特异性鉴定
|
摘要
以101份南方花生区试品种为材料,利用形态学性状和SSR标记进行品种遗传多样性分析和特异性鉴定。结果表明, 29个形态学性状中有7个无多样性,其余22个的多样性指数为0.23～0.77,平均为0.43。在相似系数为0.76处,将供试品种划分为七大类群,同一育种单位的品种倾向于聚在一起。用40个SSR标记共检测出167个等位基因,单个标记检测的等位基因数2～6个,平均为4.18个。标记的多态性信息量(PIC)差异较大,最大为0.79,最小为0.26,平均为0.55。在相似系数为0.70处,供试品种可被划分为六大类群,同一省份育成的品种多聚为一类。Mantel检验发现品种间的形态学性状和SSR标记的相似系数矩阵相关性弱(r=0.36),SSR标记无法取代形态学性状单独用于花生品种特异性鉴定,但两者相结合能有效提高花生品种特异性鉴定的准确性。
The genetic diversity and distinctness of 101 peanut varieties participated in the South China peanut field trial were analyzed based on their morphological traits and SSR markers. Among 29 morphological traits seven were no difference while 22 demonstrated diversity indexes ranging from 0.23 to 0.77, with an average of 0.43. The varieties were divided into seven groups at the similarity coefficient of 0.76, and those released by the same breeding institution tended to converge into one cluster. Molecular characterization with 40 highly informative SSRs generated a total of 167 alleles ranging from 2 to 6(averaged 4.18) alleles per marker. The polymorphism information content(PIC) of these markers varied from 0.79 to 0.26 with an average of 0.55/marker. The varieties were divided into six groups based on the similarity coefficient of 0.70, and those released by the same province tended to converge into one cluster. Mantel testing revealed that the correlations of the similarity coefficient matrixes between the morphological traits and SSR markers were weak(r = 0.36), implying that SSR markers are unable to replace morphological traits to be solely adopted to identify the distinctness of peanut varieties, but the combination of morphological traits and SSR markers will effectively increase the accuracy of distinctiveness identification.
The genetic diversity and distinctness of 101 peanut varieties participated in the South China peanut field trial were analyzed based on their morphological traits and SSR markers. Among 29 morphological traits seven were no difference while 22 demonstrated diversity indexes ranging from 0.23 to 0.77, with an average of 0.43. The varieties were divided into seven groups at the similarity coefficient of 0.76, and those released by the same breeding institution tended to converge into one cluster. Molecular characterization with 40 highly informative SSRs generated a total of 167 alleles ranging from 2 to 6(averaged 4.18) alleles per marker. The polymorphism information content(PIC) of these markers varied from 0.79 to 0.26 with an average of 0.55/marker. The varieties were divided into six groups based on the similarity coefficient of 0.70, and those released by the same province tended to converge into one cluster. Mantel testing revealed that the correlations of the similarity coefficient matrixes between the morphological traits and SSR markers were weak(r = 0.36), implying that SSR markers are unable to replace morphological traits to be solely adopted to identify the distinctness of peanut varieties, but the combination of morphological traits and SSR markers will effectively increase the accuracy of distinctiveness identification.
引文
[1]中华人民共和国农业农村部种子管理局.中国种业大数据平台.北京:中华人民共和国农业农村部,2017[2018-04-01].http://202.127.42.47:6009/Home/BigDataIndex.Bureau of Seed Management of Ministry of Agriculture and Rural Affairs of the People’s Republic of China.China Seed Industry Big Data Platform.Beijing:Ministry of Agriculture and Rural Affairs of the People’s Republic of China,2017[2018-04-01].http://202.127.42.47:6009/Home/BigDataIndex.
[2]中华人民共和国国务院.中华人民共和国植物新品种保护条例.北京:中国农业出版社,1997.pp 4-5.The State Council of the People’s Republic of China.Regulations of the People’s Republic of China Plant Variety Protection.Beijing:China Agriculture Press,1997.pp 4-5(in Chinese).
[3]鲁清,李少雄,陈小平,周桂元,洪彦彬,李海芬,梁炫强.我国南方产区花生育种现状、存在问题及育种建议.中国油料作物学报,2017,39:556-566.Lu Q,Li S X,Chen X P,Zhou G Y,Hong Y B,Li H F,Liang XQ.Current situation,problems and suggestions of peanut breeding in southern China.Chin J Oil Crop Sci,2017,39:556-566(in Chinese with English abstract).
[4]Hayward A C,Tollenaere R,Dalton-Morgan J,Batley J.Molecular marker applications in plants.Methods Mol Biol,2015,1245:13-27.
[5]Cockram J,Jones H,Norris C,O'Sullivan D M.Evaluation of diagnostic molecular markers for DUS phenotypic assessment in the cereal crop,barley(Hordeum vulgare ssp.vulgare L.).Theor Appl Genet,2012,125:1735-1749
[6]Jones H,Norris C,Smith D,Cockram J,Lee D,O’Sullivan D M,Mackay I.Evaluation of the use of high-density SNP genotyping to implement UPOV Model 2 for DUS testing in barley.Theor Appl Genet,2013,126:901-911.
[7]Tommasini L,Batley J,Arnold G M,Cooke R J,Donini P,Lee D,Law J R,Lowe C,Moule C,Trick M,Edwards K J.The development of multiplex simple sequence repeat(SSR)markers to complement distinctness,uniformity and stability testing of rape(Brassica napus L.)varieties.Theor Appl Genet,2003,106:1091-1101.
[8]Arens P,Mansilla C,Deinum D,Cavellini L,Moretti A,Rolland S,van der Schoot H,Calvache D,Ponz F,Collonnier C,Mathis R,Smilde D,Caranta C,Vosman B.Development and evaluation of robust molecular markers linked to disease resistance in tomato for distinctness,uniformity and stability testing.Theor Appl Genet,2010,120:655-664.
[9]Jones H,Mackay I.Implications of using genomic prediction within a high-density SNP dataset to predict DUS traits in barley.Theor Appl Genet,2015,128:2461-2470.
[10]滕海涛,吕波,赵久然,徐岩,王凤格,堵苑苑,杨坤,唐浩,李祥羽.利用DNA指纹图谱辅助植物新品种保护的可能性.生物技术通报,2009,(1):1-6.Teng H T,Lyu B,Zhao J R,Xu Y,Wang F G,Du Y Y,Yang K,Tang H,Li X Y.DNA fingerprint profile involved in plant variety protection practice.Biotechnol Bull,2009,(1):1-6(in Chinese with English abstract).
[11]中华人民共和国农业农村部.植物新品种特异性、一致性和稳定性测试指南:花生.北京:中国农业出版社,2012.pp 4-7.Ministry of Agriculture and Rural Affairs of the People’s Republic of China.Guidelines for the Conduct of Tests for Distinctness,Uniformity and Stability:Peanut(Arachis hypogaea L.).Beijing:China Agriculture Press,2012.pp 4-7(in Chinese).
[12]Shirasawa K,Bertioli D J,Varshney R K,Moretzsohn M C,Leal-Bertioli S C M,Thudi M,Pandey M K,Rami J F,Foncéka D,Gowda M V C,Qin H,Guo B,Hong Y,Liang X,Hirakawa H,Tabata S,Isobe S.Integrated consensus map of cultivated peanut and wild relatives reveals structures of the A and B genomes of Arachis and divergence of the legume genomes.DNA Res,2013,20:173-184.
[13]Heilmann-Clausen J,Christensen M,Fr?slev T G,Kj?ller R.Taxonomy of Tricholoma in northern Europe based on ITSsequence data and morphological characters.Persoonia,2017,38:38-57.
[14]Smykal P,Horacek J,Dostalova R,Hybl M.Variety discrimination in pea(Pisum sativum L.)by molecular,biochemical and morphological markers.J Appl Genet,2008,49:155-166.
[15]Ebrahimi A,Zarei A,Zamani F M,Lawson S.Evaluation of genetic variability among“Early Mature”Juglans regia using microsatellite markers and morphological traits.Peer J,2017,5:e3834.
[16]Rebaa F,Abid G,Aouida M,Abdelkarim S,Aroua I,Muhovski Y,Baudoin J P,M’hamdi M,Sassi K,Jebara M.Genetic variability in Tunisian populations of faba bean(Vicia faba L.var.major)assessed by morphological and SSR markers.Physiol Mol Biol Plants,2017,23:397-409.
[17]李瑞峰,高鹏,朱子成,栾非时.基于形态学标记及SSR标记的甜瓜主栽品种分类鉴定研究.中国蔬菜,2014,(6):20-27.Li R F,Gao P,Zhu Z C,Luan F S.Studies on classification and identification based on morphological markers and SSR markers for elite varieties of Cucumis melo L.China Vegetables,2014,(6):20-27(in Chinese with English abstract).
[18]李鹤,郭世荣,束胜,徐扬,孙锦.砧用南瓜种质资源形态学性状与SSR标记分析.园艺学报,2014,41:1379-1390.Li H,Guo S R,Shu S,Xu Y,Sun J.Germplasm resources analysis of rootstock-used pumpkins by phenotype and SSR.Acta Hortic Sin,2014,41:1379-1390(in Chinese with English abstract).
[2]中华人民共和国国务院.中华人民共和国植物新品种保护条例.北京:中国农业出版社,1997.pp 4-5.The State Council of the People’s Republic of China.Regulations of the People’s Republic of China Plant Variety Protection.Beijing:China Agriculture Press,1997.pp 4-5(in Chinese).
[3]鲁清,李少雄,陈小平,周桂元,洪彦彬,李海芬,梁炫强.我国南方产区花生育种现状、存在问题及育种建议.中国油料作物学报,2017,39:556-566.Lu Q,Li S X,Chen X P,Zhou G Y,Hong Y B,Li H F,Liang XQ.Current situation,problems and suggestions of peanut breeding in southern China.Chin J Oil Crop Sci,2017,39:556-566(in Chinese with English abstract).
[4]Hayward A C,Tollenaere R,Dalton-Morgan J,Batley J.Molecular marker applications in plants.Methods Mol Biol,2015,1245:13-27.
[5]Cockram J,Jones H,Norris C,O'Sullivan D M.Evaluation of diagnostic molecular markers for DUS phenotypic assessment in the cereal crop,barley(Hordeum vulgare ssp.vulgare L.).Theor Appl Genet,2012,125:1735-1749
[6]Jones H,Norris C,Smith D,Cockram J,Lee D,O’Sullivan D M,Mackay I.Evaluation of the use of high-density SNP genotyping to implement UPOV Model 2 for DUS testing in barley.Theor Appl Genet,2013,126:901-911.
[7]Tommasini L,Batley J,Arnold G M,Cooke R J,Donini P,Lee D,Law J R,Lowe C,Moule C,Trick M,Edwards K J.The development of multiplex simple sequence repeat(SSR)markers to complement distinctness,uniformity and stability testing of rape(Brassica napus L.)varieties.Theor Appl Genet,2003,106:1091-1101.
[8]Arens P,Mansilla C,Deinum D,Cavellini L,Moretti A,Rolland S,van der Schoot H,Calvache D,Ponz F,Collonnier C,Mathis R,Smilde D,Caranta C,Vosman B.Development and evaluation of robust molecular markers linked to disease resistance in tomato for distinctness,uniformity and stability testing.Theor Appl Genet,2010,120:655-664.
[9]Jones H,Mackay I.Implications of using genomic prediction within a high-density SNP dataset to predict DUS traits in barley.Theor Appl Genet,2015,128:2461-2470.
[10]滕海涛,吕波,赵久然,徐岩,王凤格,堵苑苑,杨坤,唐浩,李祥羽.利用DNA指纹图谱辅助植物新品种保护的可能性.生物技术通报,2009,(1):1-6.Teng H T,Lyu B,Zhao J R,Xu Y,Wang F G,Du Y Y,Yang K,Tang H,Li X Y.DNA fingerprint profile involved in plant variety protection practice.Biotechnol Bull,2009,(1):1-6(in Chinese with English abstract).
[11]中华人民共和国农业农村部.植物新品种特异性、一致性和稳定性测试指南:花生.北京:中国农业出版社,2012.pp 4-7.Ministry of Agriculture and Rural Affairs of the People’s Republic of China.Guidelines for the Conduct of Tests for Distinctness,Uniformity and Stability:Peanut(Arachis hypogaea L.).Beijing:China Agriculture Press,2012.pp 4-7(in Chinese).
[12]Shirasawa K,Bertioli D J,Varshney R K,Moretzsohn M C,Leal-Bertioli S C M,Thudi M,Pandey M K,Rami J F,Foncéka D,Gowda M V C,Qin H,Guo B,Hong Y,Liang X,Hirakawa H,Tabata S,Isobe S.Integrated consensus map of cultivated peanut and wild relatives reveals structures of the A and B genomes of Arachis and divergence of the legume genomes.DNA Res,2013,20:173-184.
[13]Heilmann-Clausen J,Christensen M,Fr?slev T G,Kj?ller R.Taxonomy of Tricholoma in northern Europe based on ITSsequence data and morphological characters.Persoonia,2017,38:38-57.
[14]Smykal P,Horacek J,Dostalova R,Hybl M.Variety discrimination in pea(Pisum sativum L.)by molecular,biochemical and morphological markers.J Appl Genet,2008,49:155-166.
[15]Ebrahimi A,Zarei A,Zamani F M,Lawson S.Evaluation of genetic variability among“Early Mature”Juglans regia using microsatellite markers and morphological traits.Peer J,2017,5:e3834.
[16]Rebaa F,Abid G,Aouida M,Abdelkarim S,Aroua I,Muhovski Y,Baudoin J P,M’hamdi M,Sassi K,Jebara M.Genetic variability in Tunisian populations of faba bean(Vicia faba L.var.major)assessed by morphological and SSR markers.Physiol Mol Biol Plants,2017,23:397-409.
[17]李瑞峰,高鹏,朱子成,栾非时.基于形态学标记及SSR标记的甜瓜主栽品种分类鉴定研究.中国蔬菜,2014,(6):20-27.Li R F,Gao P,Zhu Z C,Luan F S.Studies on classification and identification based on morphological markers and SSR markers for elite varieties of Cucumis melo L.China Vegetables,2014,(6):20-27(in Chinese with English abstract).
[18]李鹤,郭世荣,束胜,徐扬,孙锦.砧用南瓜种质资源形态学性状与SSR标记分析.园艺学报,2014,41:1379-1390.Li H,Guo S R,Shu S,Xu Y,Sun J.Germplasm resources analysis of rootstock-used pumpkins by phenotype and SSR.Acta Hortic Sin,2014,41:1379-1390(in Chinese with English abstract).