同一SSR引物在不同棉种BAC文库中的筛选及其FISH分析
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摘要
本实验室前期利用SSR引物NAU1201筛选海岛棉Pima 90-53 BAC文库,其中BAC克隆299N22含有特殊的重复序列。本试验用SSR引物NAU1201分别在阿非利加棉文库和达尔文氏棉文库中筛选到3个和1个阳性BAC克隆。这些BAC克隆在A(亚)组所有的染色体上均有弥散的杂交信号,而在D(亚)组所有的染色体上几乎没有杂交信号,这与海岛棉文库BAC克隆299N22的FISH结果一致。相似的FISH杂交信号说明这些BAC克隆极有可能均含有相同的重复序列。这些BAC克隆的获得为研究该重复序列在不同棉种基因组中的分布情况以及序列差异提供了物质基础。
引文
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[2]Hendrix B,Stewart J M.Estimation of the nuclear DNAcontent of Gossypium species[J].Annals of Botany,2005,95(5):789-797.
[3]Jurka J,Kapitonov V V,Kohany O,et al.Repetitive sequences in complex genomes:structure and evolution[J].Annual Review of Genomics and Human Genetics,2007,8:241-259.
[4]Wessler S R.Transposable elements and the evolution of eukaryotic genomes[J].Proceedings of the National Academy of Sciences,2006,103(47):17600-17601.
[5]Wang K B,Wang Z W,Li F G,et al.The draft genome of a diploid cotton Gossypium raimondii[J].Nature Genetics,2012,44(10):1098-1103.
[6]Li F G,Fan G Y,Wang K B,et al.Genome sequence of the cultivated cotton Gossypium arboreum[J].Nature Genetics,2014,46(6):567-572.
[7]Zhao X P,Si Y,Hanson R E,et al.Dispersed repetitive DNA has spread to new genomes since polyploid formation in cotton[J].Genome Research,1998,8(5):479-492.
[8]Wendel J F,Cronn R C.Polyploidy and the evolutionary history of cotton[J].Advances in Agronomy,2003,78:139-186.
[9]Wendel J F.Genome evolution in ployploids[M]//Doyle JJ,Gaut B C.Plant molecular evolution.Dordrecht:Springer,2000:225-249.
[10]Cui X L,Liu F,Liu Y L,et al.Screening and chromosome localization of two cotton BAC clones[J].Comparative Cytogenetics,2016,10(1):1-15.
[11]高海燕.阿非利加棉细菌人工染色体文库的构建[D].北京:中国农业科学院,2012.
[12]宋国立,崔荣霞.改良CTAB法快速提取棉花DNA[J].棉花学报,1998,10(5):273-275.
[13]程华,彭仁海,张香娣,等.棉花BAC文库快速筛选法[J].生物技术,2012(3):55-57.
[14]Sambrook J,Fritsch E F,Maniatis T.Molecular cloning:Alaboratory manual[M].2nd ed.New York:Cold Spring Harbor Laboratory Press,1989.
[15]崔兴雷,徐俊民,刘玉玲,等.雷蒙德氏棉一段单拷贝序列的FISH分析[J].棉花学报,2016,28(2):179-183.
[16]Lu H J,Cui X L,Liu Z,et al.Discovery and annotation of a novel transposable element family in Gossypium[J].BMC Plant Biology,2018,18(1):307.
[17]Grover C E,Kim H,Wing R A,et al.Incongruent patterns of local and global genome size evolution in cotton[J].Genome Research,2004,14(8):1474-1482.