灭草烟在甘蔗组培苗生根阶段浓度筛选试验
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摘要
以ROC22增殖阶段的组培苗为材料,探究了不同浓度灭草烟处理对小苗生根及生长的影响。灭草烟处理浓度为0.5μmol/L时,生根率低于50%,小苗没有假茎,绿叶数量显著减少。处理浓度为1.2μmol/L以上时,生根率为0。当处理浓度为1.6～2.5μmol/L时,接近半致死浓度。综合致死率和苗的生根及生长情况分析,灭草烟浓度为1.2μmol/L时,ROC22组培苗能部分存活但不能长根。因此,适合ROC22组培苗生根阶段的灭草烟浓度为1.2μmol/L。
With the callus growth of sugarcane variety ROC22 as materials, this paper determined the concentration of imazapyr in rooting stage of ROC22. The results showed that the rooting plantlet percentage of the sugarcane was less than 50% when the imazapyr concentration was 0.5 μmol/L, and the plantlet had no pseudo-stem and much less green leaves. The rooting plantlet percentage was zero when the imazapyr concentration was 1.2 μmol/L. As the imazapyr concentration was 1.6～2.5 μmol/L, it was close to the semi-lethal concentration. Based on some factors such as fatality rate and rooting ratio as well as plantlet growth,the suitable imazapyr concentration at the rooting stage for ROC22 was 1.2 μmol/L.
With the callus growth of sugarcane variety ROC22 as materials, this paper determined the concentration of imazapyr in rooting stage of ROC22. The results showed that the rooting plantlet percentage of the sugarcane was less than 50% when the imazapyr concentration was 0.5 μmol/L, and the plantlet had no pseudo-stem and much less green leaves. The rooting plantlet percentage was zero when the imazapyr concentration was 1.2 μmol/L. As the imazapyr concentration was 1.6～2.5 μmol/L, it was close to the semi-lethal concentration. Based on some factors such as fatality rate and rooting ratio as well as plantlet growth,the suitable imazapyr concentration at the rooting stage for ROC22 was 1.2 μmol/L.
引文
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[13]何慧怡,樊丽娜,齐永文,等.灭草烟在甘蔗愈伤分化阶段和刚成苗阶段浓度筛选试验[J].广东农业科学,2015(5):49-52.
[14]李庚虎,安飞飞,陈霆,等.除草剂Basta对11个木薯品种(品系)组培苗生长的影响[J].热带作物学报,2013,34(4):681-684.
[15]罗素兰,陈若,长孙东亭,等.番茄组培苗的不同阶段对抗生素和PPT的抗性筛选试验[J].海南大学学报(自然科学版),2003,21(1):58-64.
[16]罗素兰,林皎月,长孙东亭,等.甘蔗组织培养中不同阶段的抗生素及PPT的抗性筛选试验[J].海南大学学报(自然科学版),2003,21(3):259-264.
[17]张敏,杨素欣,邵群,等.一种安全高效大豆转化筛选体系的建立[J].生物技术通报,2012(5):66-70.
[18]王罡,吴静叶,王萍,等.马铃薯组培苗对除草剂的耐性[J].贵州农业科学,2014,42(9):116-119.
[2]段维兴,刘许辉,杨海霞,等.4种除草剂对甘蔗新品种产量及构成因素的影响[J].作物研究,2012,26(5):504-507.
[3]赵勇,昝逢刚,罗志明,等.除草剂在甘蔗生产中的应用研究进展[J].中国糖料,2017,39(2):54-57,70.
[4]姚敦团.3种除草剂除草效果及对甘蔗生长的影响[J].农业研究与应用,2015(3):64-65,69.
[5]王学东,欧晓明,樊德方.除草剂咪唑烟酸的环境行为研究进展[J].世界农药,2002,24(6):4,15-17.
[6]王学东,欧晓明,王慧利,等.除草剂咪唑烟酸在土壤中的微生物降解研究[J].土壤学报,2004,41(1):156-159.
[7]TAN S,EVANS R R,DAHMER M L,et a1.Imidazolinone-tolerant crops:history,current status and future[J].Pest Management Science,2005,61(3):246-257.
[8]张敏,杨素欣,邵群,等.一种安全高效大豆转化筛选体系的建立[J].生物技术通报,2012(5):66-70.
[9]王伦旺.广西甘蔗理化诱变育种研究回顾与展望[J].广西蔗糖,2002(2):7-9.
[10]安学丽,蔡一林,王久光,等.化学诱变及其在农作物育种上应用[J].核农学报,2003,17(3):239-242.
[11]KOCH A C,RAMGAREEB S,RUTHERFORD R S,et a1.An in vitro mutagenesis protocol for the production of sugarcane tolerant to the herbicide imazapyr[J].In Vitro Cell Dev-Pl,2012,48(4):417-427.
[12]甘仪梅,张树珍,曾凡云,等.甘蔗转基因育种研究进展[J].生物技术通报,2013(3):1-9.
[13]何慧怡,樊丽娜,齐永文,等.灭草烟在甘蔗愈伤分化阶段和刚成苗阶段浓度筛选试验[J].广东农业科学,2015(5):49-52.
[14]李庚虎,安飞飞,陈霆,等.除草剂Basta对11个木薯品种(品系)组培苗生长的影响[J].热带作物学报,2013,34(4):681-684.
[15]罗素兰,陈若,长孙东亭,等.番茄组培苗的不同阶段对抗生素和PPT的抗性筛选试验[J].海南大学学报(自然科学版),2003,21(1):58-64.
[16]罗素兰,林皎月,长孙东亭,等.甘蔗组织培养中不同阶段的抗生素及PPT的抗性筛选试验[J].海南大学学报(自然科学版),2003,21(3):259-264.
[17]张敏,杨素欣,邵群,等.一种安全高效大豆转化筛选体系的建立[J].生物技术通报,2012(5):66-70.
[18]王罡,吴静叶,王萍,等.马铃薯组培苗对除草剂的耐性[J].贵州农业科学,2014,42(9):116-119.