高氧液对油酸性急性肺损伤家兔肺组织改变和肺表面活性物质的影响
|
摘要
目的观察高氧液对急性油酸性肺损伤家兔肺组织形态改变及肺表面活性物质的影响。方法健康家兔30只,雌雄不拘,体质量2.0~2.5kg,随机分为3组:空白对照组(n=10);高氧液治疗组(n=10);盐水对照组(n=10)。3组动物给予相应处理后分别在ALI模型制备前(基础值)、静脉输液前(损伤后)、输液后30min、1h、2h时观察动脉血气变化,2h后处死动物取肺组织做病理切片观察肺组织形态学改变和免疫组化检测肺表面活性物质的变化。结果高氧液治疗组在输注高氧液后2hPaO2明显高于盐水对照组(P<0.001),而PaCO2则低于盐水对照组(P<0.001)。光镜下高氧液治疗组家兔肺组织损伤程度较盐水对照组轻。免疫组化及肺泡灌洗液测定结果显示,高氧液治疗组肺表面活性物质A含量明显高于盐水对照组(P<0.001)。结论静脉输注高氧液可减轻肺组织损伤程度,减少肺泡表面活性物质的破坏。
Objective To observe the effects of hyperoxia solution on pulmonary surfactant and histology of rabbits with oleic acid-induced acute lung injury.Methods Thirty healthy rabbits weighing 2.0-2.5 kg were randomly divided into three groups with 10 rabbits each:control group,hyperoxia solution treatment group,and saline treatment group.Blood samples were taken for blood-gas analysis before and at 30,60 and120 min after oleic acid or normal saline administration.Two hours later,the animals were killed and pathologic changes of lung tissue were observed microscopically.The expression of SP-A was investigated using the immunohistochemical method and image analysis system.Results PaO2 was significantly higher in hyperoxia solution treatment group than in saline treatment group two hours after treatment(P<0.001),but PaCO2 was significantly lower than that in saline treatment group(P<0.001).Optical microscopy showed that lung tissue damage was milder in hyperoxia solution treatment group than in saline treatment group.The content of surfactant A in hyperoxia solution treatment group was significantly greater than that in saline treatment group(P<0.001).Conclusion Hyperoxia solution can lessen lung tissue injury and damage to pulmonary surfactant.
Objective To observe the effects of hyperoxia solution on pulmonary surfactant and histology of rabbits with oleic acid-induced acute lung injury.Methods Thirty healthy rabbits weighing 2.0-2.5 kg were randomly divided into three groups with 10 rabbits each:control group,hyperoxia solution treatment group,and saline treatment group.Blood samples were taken for blood-gas analysis before and at 30,60 and120 min after oleic acid or normal saline administration.Two hours later,the animals were killed and pathologic changes of lung tissue were observed microscopically.The expression of SP-A was investigated using the immunohistochemical method and image analysis system.Results PaO2 was significantly higher in hyperoxia solution treatment group than in saline treatment group two hours after treatment(P<0.001),but PaCO2 was significantly lower than that in saline treatment group(P<0.001).Optical microscopy showed that lung tissue damage was milder in hyperoxia solution treatment group than in saline treatment group.The content of surfactant A in hyperoxia solution treatment group was significantly greater than that in saline treatment group(P<0.001).Conclusion Hyperoxia solution can lessen lung tissue injury and damage to pulmonary surfactant.
引文
[1]MORALES MM,PIRESNETO RC,INFORSATO N,et al.Small airway remodeling in acute respiratory distress syndrome:a study in autopsy lung tissue[J].Crit Care,2011,15(1):R4.
[2]刘笑玎,原铭贞,王司仪,等.水通道蛋白1、3、4和5与急性肺损伤关系的研究进展[J].吉林大学学报:医学版,2014,40(5):1119-1122.
[3]PIRESNETO RC,MORALES MM,LANCAS T,et al.Expression of acute-phase cytokines,surfactant proteins,and epithelial apoptosis in small airways of human acute respiratory distress syndrome[J].J Crit Care,2013,28(1):111.e9-111e.15.
[4]徐瑞芬,徐礼鲜,徐世荣,等.高氧液对家兔油酸型急性肺损伤的治疗作用[J].第四军医大学学报,2003,24(16):1451-1453.
[5]徐礼鲜,吴利平,张惠,等.高氧液提高乏氧血氧含量及相关基础研究[J].中国药理学通报,2002,18(5):560-562.
[6]GOTO H,LEDFORD JG,MUKHERIEE S,et al.The role of surfactant protein A in bleomycin-induced acute lung injury[J].Am J Respir Crit Care Med,2010,181(12):1336-1344.
[7]王亿胜,李宏云,冯永海,等.小潮气量通气对急性肺损伤/急性呼吸窘迫综合征高风险患者疗效及预后的影响[J].郑州大学学报:医学版,2014,49(3):393-397.
[8]HOLLENHORST MI,RICHTER K,FRONIUS M.Ion transport by pulmonary epithelia[J].J Biomed Biotechnol,2011,2011:174306.
[9]MASON RJ.Biology of alveolar typeⅡcell[J].Respirology,2006,11(Suppl):12-15.
[10]WRIGHT JR.Immunoregulatory functions of surfactant proteins[J].Nat Rev Immunol,2005,5(1):58-68.
[11]程青,李军尧,邬明.高氧液对急性肺损伤家兔肺组织AQP-1表达的影响[J].西北国防医学杂志,2010,28(3):195-197.
[12]何琳,李涛平,朱丽华.油酸肺损伤时肺泡内液体清除和肺泡Ⅱ型细胞内cAMP、cGMP水平的变化[J].南方医科大学学报,2008,28(4):513-515.
[13]乔莉娟,王立贤,颜华,等.猪肺表面活性蛋白A在急性肺损伤的变化及其意义[J].中国农业科学,2010,43(14):3027-3035.
[13]赵晖,柴伟,孙绪德,等.高氧液对模拟高原急性缺氧所致氧化性损伤的保护作用[J].临床麻醉学杂志,2010,26(7):613-615.
[14]陈隽鹏,洪丰,彭建明,等.高氧液对急性呼吸窘迫综合征大鼠的治疗作用[J].广东医学,2007,28(2):209-210.
[15]张晓荣,孙胜,史连胜,等.高氧液在高原肺水肿早期救治中的临床研究[J].华南国防医学杂志,2013,28(9):678-679.
[2]刘笑玎,原铭贞,王司仪,等.水通道蛋白1、3、4和5与急性肺损伤关系的研究进展[J].吉林大学学报:医学版,2014,40(5):1119-1122.
[3]PIRESNETO RC,MORALES MM,LANCAS T,et al.Expression of acute-phase cytokines,surfactant proteins,and epithelial apoptosis in small airways of human acute respiratory distress syndrome[J].J Crit Care,2013,28(1):111.e9-111e.15.
[4]徐瑞芬,徐礼鲜,徐世荣,等.高氧液对家兔油酸型急性肺损伤的治疗作用[J].第四军医大学学报,2003,24(16):1451-1453.
[5]徐礼鲜,吴利平,张惠,等.高氧液提高乏氧血氧含量及相关基础研究[J].中国药理学通报,2002,18(5):560-562.
[6]GOTO H,LEDFORD JG,MUKHERIEE S,et al.The role of surfactant protein A in bleomycin-induced acute lung injury[J].Am J Respir Crit Care Med,2010,181(12):1336-1344.
[7]王亿胜,李宏云,冯永海,等.小潮气量通气对急性肺损伤/急性呼吸窘迫综合征高风险患者疗效及预后的影响[J].郑州大学学报:医学版,2014,49(3):393-397.
[8]HOLLENHORST MI,RICHTER K,FRONIUS M.Ion transport by pulmonary epithelia[J].J Biomed Biotechnol,2011,2011:174306.
[9]MASON RJ.Biology of alveolar typeⅡcell[J].Respirology,2006,11(Suppl):12-15.
[10]WRIGHT JR.Immunoregulatory functions of surfactant proteins[J].Nat Rev Immunol,2005,5(1):58-68.
[11]程青,李军尧,邬明.高氧液对急性肺损伤家兔肺组织AQP-1表达的影响[J].西北国防医学杂志,2010,28(3):195-197.
[12]何琳,李涛平,朱丽华.油酸肺损伤时肺泡内液体清除和肺泡Ⅱ型细胞内cAMP、cGMP水平的变化[J].南方医科大学学报,2008,28(4):513-515.
[13]乔莉娟,王立贤,颜华,等.猪肺表面活性蛋白A在急性肺损伤的变化及其意义[J].中国农业科学,2010,43(14):3027-3035.
[13]赵晖,柴伟,孙绪德,等.高氧液对模拟高原急性缺氧所致氧化性损伤的保护作用[J].临床麻醉学杂志,2010,26(7):613-615.
[14]陈隽鹏,洪丰,彭建明,等.高氧液对急性呼吸窘迫综合征大鼠的治疗作用[J].广东医学,2007,28(2):209-210.
[15]张晓荣,孙胜,史连胜,等.高氧液在高原肺水肿早期救治中的临床研究[J].华南国防医学杂志,2013,28(9):678-679.