白屈菜红碱抑制肝星状细胞活化和胶原合成
摘要
目的研究白屈菜红碱对大鼠肝星状细胞(HSC-T6)的增殖和胶原合成的影响。方法利用MTT法检测乙醛活化的HSC-T6的细胞活力,消化法检测细胞羟脯氨酸(Hyp)含量,ELISA法检测细胞上清液中Ⅰ型胶原蛋白(Col-Ⅰ)和Ⅲ型胶原蛋白(Col-Ⅲ),RT-PCR检测α-平滑肌肌动蛋白(α-SMA)mRNA的表达。结果与正常组比较,模型组HSC-T6的细胞增殖、Hyp含量、Col-Ⅰ和Col-Ⅲ含量较正常对照组均显著升高(P<0.05),α-SMA mRNA表达明显上调(P<0.05)。而与模型组比较,模型+白屈菜红碱(10μg·ml~(-1),20μg·ml~(-1))组HSC-T6的细胞增殖、Hyp含量、Col-Ⅰ和Col-Ⅲ含量显著降低(P<0.05),α-SMA mRNA的表达明显下调(P<0.05)。结论白屈菜红碱抑制大鼠肝星状细胞的增殖和胶原合成。
引文
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[3] Miller AM,Wang H,Park O,et al.Anti-inflammatory and antiapoptotic roles of endothelial cell STAT3 in alcoholic liver injury[J].Alcohol Clin Exp Res,2010,34(4):719-725.
[4] Klassen LW,Thiele GM,Duryee MJ,et al.An in vitro method of alcoholic liver injury using precision cut liver slices from rats[J].Biochem Pharmacol,2008,76(3):426-436.
[5]王晓丽,郭丽娜,孔寰宇,等.齐齐哈尔地区白屈菜中白屈菜红碱的含量测定[J].齐齐哈尔医学院学报,2014,35(16):2441-2441.
[6]李晓明,欧阳婷庭,董妙先,等.白屈菜红碱对肝纤维化小鼠TGF-β/Smads信号通路的影响[J].中国病理生理杂志,2018,34(7):1323-1328.
[7]王晓丽,郭丽娜,孙辑凯,等.齐齐哈尔地区野生白屈菜的初步质量考察[J].齐齐哈尔医学院学报,2014,35(14):2031-2032.
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