大黄煨制前后对正常大鼠胃肠功能的影响
|
摘要
目的:探讨大黄对正常大鼠胃肠功能的影响及其作用机制,并比较其煨制前后作用差异。方法:将健康SD大鼠随机分为空白组、生大黄高、中、低剂量组(1.5,3.0,6.0 g·kg~(-1)·d~(-1))以及煨大黄高、中、低剂量组(给药剂量同生品)共7组,每组8只,连续灌胃给药10 d,通过大鼠的体质量、进食量、胃内容物残留率、小肠推进率、胃系数以及胃组织形态观察评价大黄对胃肠功能的影响;采用酶联免疫吸附测定法(ELISA)检测大鼠血浆胃动素(MTL),血清胃泌素(GAS),肿瘤坏死因子(TNF)-α,白细胞介素-10(IL-10)的含量,探究大黄对胃肠功能产生影响的作用机制,比较大黄煨制前后对正常大鼠胃肠功能的影响。结果:各给药组不同程度的减缓了大鼠体质量增长,抑制胃排空、小肠推进,引发炎症反应,增加胃黏膜损伤。与空白组相比,除煨大黄低、中剂量组外,其他各组大鼠胃内残留率、胃系数和TNF-α含量显著升高(P<0.05,P<0.01);IL-10含量显著降低(P<0.01);除煨大黄低剂量组外,其他各组大鼠体质量,小肠推进率,GAS和MTL含量显著降低(P<0.05,P<0.01)。与等剂量生大黄组相比,煨大黄各剂量组大鼠体质量,小肠推进率,GAS,IL-10,MTL含量均有不同程度的升高;胃系数、胃内容物残留率和TNF-α含量均有不同程度的降低。结论:大黄导致胃肠功能紊乱的原因可能与其抑制胃肠激素和促进炎症细胞因子的表达有关;大黄煨制后,大黄"苦寒败胃"的作用减弱,为大黄的临床合理用药提供了实验依据。
Objective:To investigate the effect of Rhei Radix et Rhizoma on gastrointestinal function of normal rats and explore its therapeutic mechanism,and compare the difference of this herb before and after simmering.Method:Healthy SD rats were randomly divided into 7 groups,including the blank group,the high,medium and low dose groups of raw Rhei Radix et Rhizoma(1.5,3.0,6.0 g·kg~(-1)·d~(-1))and the high,medium and low dose groups of simmered Rhei Radix et Rhizoma(1.5,3.0,6.0 g·kg~(-1)·d~(-1)),eight rats in each group were continuous intragastric administration for 10 days,the effect of Rhei Radix et Rhizoma on gastrointestinal function of rats were evaluated by body weight,food intake,gastric remnant rate,small intestine propelling rate,stomach weight coefficient and gastrointestinal tissue morphology;enzyme-linked immunosorbent assay(ELISA)was used to detect contents of motilin(MTL)in rat plasma and gastrin(GAS),tumor necrosis factor-α(TNF-α),interleukin-10(IL-10)in rat serum,the mechanism of effect of Rhei Radix et Rhizoma on gastrointestinal function was explored and the effect of Rhei Radix et Rhizoma on gastrointestinal function of normal rats before and after simmering was compared.Result:In each administration group,weight growth of rats was slowed down,gastric emptying and intestinal propulsion were inhibited,inflammatory reaction was triggered,and gastric mucosal injury was increased.Compared with the blank group,except for the low-dose and medium-dose groups of simmered Rhei Radix et Rhizoma,stomach weight coefficient,gastric remnant rate and content of TNF-αof rats were significantly increased(P<0.05,P<0.01),and the content of IL-10 was significantly reduced,in addition to the low-dose group of simmered Rhei Radix et Rhizoma,the weight,small intestine propelling rate,contents of GAS and MTL of rats were significantly decreased(P<0.05,P<0.01).Compared with the isodose raw Rhei Radix et Rhizoma group,the weight,small intestine propelling rate,contents of GAS,IL-10 and MTL were all increased with different degrees,the gastric weight coefficient,gastric remnant rate and content of TNF-αof rats were all decreased to different degrees.Conclusion:Gastrointestinal dysfunction caused by Rhei Radix et Rhizoma is related to inhibiting the expression of gastrointestinal hormones and promoting the expression of inflammatory cytokines,in addition,the effect of"stomach injured by bitterness and cold property"of Rhei Radix et Rhizoma is weakened after simmering,which provides scientific basis for clinical rational use of Rhei Radix et Rhizoma.
Objective:To investigate the effect of Rhei Radix et Rhizoma on gastrointestinal function of normal rats and explore its therapeutic mechanism,and compare the difference of this herb before and after simmering.Method:Healthy SD rats were randomly divided into 7 groups,including the blank group,the high,medium and low dose groups of raw Rhei Radix et Rhizoma(1.5,3.0,6.0 g·kg~(-1)·d~(-1))and the high,medium and low dose groups of simmered Rhei Radix et Rhizoma(1.5,3.0,6.0 g·kg~(-1)·d~(-1)),eight rats in each group were continuous intragastric administration for 10 days,the effect of Rhei Radix et Rhizoma on gastrointestinal function of rats were evaluated by body weight,food intake,gastric remnant rate,small intestine propelling rate,stomach weight coefficient and gastrointestinal tissue morphology;enzyme-linked immunosorbent assay(ELISA)was used to detect contents of motilin(MTL)in rat plasma and gastrin(GAS),tumor necrosis factor-α(TNF-α),interleukin-10(IL-10)in rat serum,the mechanism of effect of Rhei Radix et Rhizoma on gastrointestinal function was explored and the effect of Rhei Radix et Rhizoma on gastrointestinal function of normal rats before and after simmering was compared.Result:In each administration group,weight growth of rats was slowed down,gastric emptying and intestinal propulsion were inhibited,inflammatory reaction was triggered,and gastric mucosal injury was increased.Compared with the blank group,except for the low-dose and medium-dose groups of simmered Rhei Radix et Rhizoma,stomach weight coefficient,gastric remnant rate and content of TNF-αof rats were significantly increased(P<0.05,P<0.01),and the content of IL-10 was significantly reduced,in addition to the low-dose group of simmered Rhei Radix et Rhizoma,the weight,small intestine propelling rate,contents of GAS and MTL of rats were significantly decreased(P<0.05,P<0.01).Compared with the isodose raw Rhei Radix et Rhizoma group,the weight,small intestine propelling rate,contents of GAS,IL-10 and MTL were all increased with different degrees,the gastric weight coefficient,gastric remnant rate and content of TNF-αof rats were all decreased to different degrees.Conclusion:Gastrointestinal dysfunction caused by Rhei Radix et Rhizoma is related to inhibiting the expression of gastrointestinal hormones and promoting the expression of inflammatory cytokines,in addition,the effect of"stomach injured by bitterness and cold property"of Rhei Radix et Rhizoma is weakened after simmering,which provides scientific basis for clinical rational use of Rhei Radix et Rhizoma.
引文
[1]国家药典委员会.中华人民共和国药典.一部[M].北京:中国医药科技出版社,2015:23-24.
[2]龚千锋.中药炮制学[M].北京:中国中医药出版社,2012:203.
[3]北京市药品监督管理局.北京市中药饮片炮制规范.上册[M].北京:化学工业出版社,2008:附录3.
[4]四川省食品药品监督管理局.四川省中药饮片炮制规范[M].成都:四川科学技术出版社,2015:515.
[5]浙江省食品药品监督管理局.浙江省中药饮片炮制规范[M].北京:中国医药科技出版社,2015:附录5.
[6]王孝涛.历代中药炮制法汇典.古代部分[M].南昌:江西科学技术出版社,1998:5-8.
[7]明·万全.万氏家传保命歌括[M].武汉:湖北科学技术出版社,1986:177,235.
[8]宋·钱乙.小儿药证直诀[M].北京:中国医药科技出版社,1998:108,126,170.
[9]张志,李听弦,姚楠,等.多指标正交试验优化大黄的湿纸煨制工艺[J].中国药房,2018,29(7):964-967.
[10]徐雯,王楠,丁浩然,等.广藿香对湿阻中焦证大鼠胃肠功能的影响[J].中国中药杂志,2017,42(23):4649-4655.
[11]林雪娇,王姝瑞,李鲜.苦寒伤阴与坚阴之探讨[J].中医研究,2018,31(6):5-7.
[12]李飞艳.从苦寒药伤胃看常用苦寒药一般毒性及对胃肠运动影响的实验研究[D].长沙:湖南中医药大学,2005.
[13]项华,章晓玲,查捷,等. SD大鼠生长发育指标和主要脏器正常参考值的探讨[J].中国卫生检验杂志,2008,18(2):299-301.
[14]刘婉青,郝菲菲,聂克.小半夏汤对小鼠胃排空和小肠推进的影响[J].辽宁中医杂志,2018,45(4):832-834.
[15]舒尊鹏,杨燕妮,王毅,等.枳壳化学拆分组分的性味药理学评价——化学拆分组分的制备及其对胃肠功能作用的影响[J].中国实验方剂学杂志,2018,24(20):14-19.
[16]高杰,曹春雨,贺蓉,等.大黄、苍术对正常大鼠胃肠激素水平的影响[J].中国实验方剂学杂志,2012,18(9):220-224.
[17]韩正贵,陆江涛,王文静,等.宣肺通腑汤辅助治疗中老年重症肺炎合并胃肠功能障碍的临床观察[J].中国实验方剂学杂志,2018,24(16):188-193.
[18]祝婧,钟凌云,叶喜德,等.枳壳不同炮制品的燥性比较及其对功能性消化不良大鼠胃肠功能的影响[J].中国实验方剂学杂志,2017,23(22):20-26.
[19]孙雄杰.苍术炒焦工艺及炒焦前后药效学与化学成分对比研究[D].武汉:湖北中医药大学,2016.
[20]闫美娟,隋峰,李燕,等.大黄各炮制品泻下作用的比较研究[J].中国实验方剂学杂志,2010,16(13):170-171.
[2]龚千锋.中药炮制学[M].北京:中国中医药出版社,2012:203.
[3]北京市药品监督管理局.北京市中药饮片炮制规范.上册[M].北京:化学工业出版社,2008:附录3.
[4]四川省食品药品监督管理局.四川省中药饮片炮制规范[M].成都:四川科学技术出版社,2015:515.
[5]浙江省食品药品监督管理局.浙江省中药饮片炮制规范[M].北京:中国医药科技出版社,2015:附录5.
[6]王孝涛.历代中药炮制法汇典.古代部分[M].南昌:江西科学技术出版社,1998:5-8.
[7]明·万全.万氏家传保命歌括[M].武汉:湖北科学技术出版社,1986:177,235.
[8]宋·钱乙.小儿药证直诀[M].北京:中国医药科技出版社,1998:108,126,170.
[9]张志,李听弦,姚楠,等.多指标正交试验优化大黄的湿纸煨制工艺[J].中国药房,2018,29(7):964-967.
[10]徐雯,王楠,丁浩然,等.广藿香对湿阻中焦证大鼠胃肠功能的影响[J].中国中药杂志,2017,42(23):4649-4655.
[11]林雪娇,王姝瑞,李鲜.苦寒伤阴与坚阴之探讨[J].中医研究,2018,31(6):5-7.
[12]李飞艳.从苦寒药伤胃看常用苦寒药一般毒性及对胃肠运动影响的实验研究[D].长沙:湖南中医药大学,2005.
[13]项华,章晓玲,查捷,等. SD大鼠生长发育指标和主要脏器正常参考值的探讨[J].中国卫生检验杂志,2008,18(2):299-301.
[14]刘婉青,郝菲菲,聂克.小半夏汤对小鼠胃排空和小肠推进的影响[J].辽宁中医杂志,2018,45(4):832-834.
[15]舒尊鹏,杨燕妮,王毅,等.枳壳化学拆分组分的性味药理学评价——化学拆分组分的制备及其对胃肠功能作用的影响[J].中国实验方剂学杂志,2018,24(20):14-19.
[16]高杰,曹春雨,贺蓉,等.大黄、苍术对正常大鼠胃肠激素水平的影响[J].中国实验方剂学杂志,2012,18(9):220-224.
[17]韩正贵,陆江涛,王文静,等.宣肺通腑汤辅助治疗中老年重症肺炎合并胃肠功能障碍的临床观察[J].中国实验方剂学杂志,2018,24(16):188-193.
[18]祝婧,钟凌云,叶喜德,等.枳壳不同炮制品的燥性比较及其对功能性消化不良大鼠胃肠功能的影响[J].中国实验方剂学杂志,2017,23(22):20-26.
[19]孙雄杰.苍术炒焦工艺及炒焦前后药效学与化学成分对比研究[D].武汉:湖北中医药大学,2016.
[20]闫美娟,隋峰,李燕,等.大黄各炮制品泻下作用的比较研究[J].中国实验方剂学杂志,2010,16(13):170-171.