芪蛭肾安丸对小鼠耳廓微循环的影响
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摘要
目的观察芪蛭肾安丸对小鼠耳廓微循环的影响,探讨其可能作用机制。方法将50只昆明种雄性小鼠通过尾静脉注射肾上腺素制造微循环障碍模型,然后随机分为芪蛭肾安丸低、中、高剂量组及尿毒清颗粒组、空白对照组5组,每组各10只。芪蛭肾安丸低、中、高剂量组分别以1.4、2.8、5.6 g/kg芪蛭肾安丸灌胃给药,尿毒清颗粒组以6.0 g/kg尿毒清颗粒灌胃给药,空白对照组予等量的蒸馏水灌胃。给药20、40min后采用冷光源分别观察记录各组小鼠右耳微动脉、微静脉管径及红细胞流速。结果各组小鼠造模后耳廓微动脉、微静脉管径及微动脉内红细胞流速与本组造模前比较差异有统计学意义(P<0.01);芪蛭肾安丸低、中、高剂量组及尿毒清颗粒组小鼠给药20、40 min后耳廓微动脉、微静脉管径及微动脉内红细胞流速与本组造模后比较差异均有统计学意义(P<0.05),空白对照组与本组造模后比较差异无统计学意义(P>0.05)。结论芪蛭肾安丸可明显增加微循环障碍模型小鼠耳廓微动脉、微静脉管径,加快微血管内红细胞流速,改善小鼠耳廓微循环,其降低蛋白尿、改善肾功能可能是通过改善微循环、增加肾血流量而实现。
Objective To study the effect of Qizhi Shenan pill on auricle microcirculation of mouse.Methods 50 Kunming male mice with microcirculatory disorders were manufactured by tail vein injection of epinephrine.Then 50 mice with microcirculatory disorders model were randomly divided into five groups,high-,middle-,low-dose Qizhi Shenan pill(received 5.6,2.8,1.4 g/kg Qizhi Shenan pill) group,Niuduqing granule group(received 6.0 g/kg Niuduqing granule) and control group(received distilled water).Microarteries,venule diameter,microvascular erythrocyte flow rate of mouse right ear were observed with cold light source after 20 and 40 min of administration.Results There was significant difference between before and after modeling on the pinna microarteries,venule diameter,microvascular erythrocyte flow rate in each group(P<0.01).There was significant difference between high-,middle-,low-dose Qizhi Shenan pill group and control group on the pinna microarteries,venule diameter,microvascular erythrocyte flow rate in each group(P<0.05).Conclusion Qizhi Shenan pill can obviously increase the pinna microarteries,venule diameter,microvascular erythrocyte flow rate of mouse with auricle microcirculation disorders model,improve auricle microcirculation of mouse,reduce proteinuria and improve renal function by improving microcirculation and increasing renal blood flow.
Objective To study the effect of Qizhi Shenan pill on auricle microcirculation of mouse.Methods 50 Kunming male mice with microcirculatory disorders were manufactured by tail vein injection of epinephrine.Then 50 mice with microcirculatory disorders model were randomly divided into five groups,high-,middle-,low-dose Qizhi Shenan pill(received 5.6,2.8,1.4 g/kg Qizhi Shenan pill) group,Niuduqing granule group(received 6.0 g/kg Niuduqing granule) and control group(received distilled water).Microarteries,venule diameter,microvascular erythrocyte flow rate of mouse right ear were observed with cold light source after 20 and 40 min of administration.Results There was significant difference between before and after modeling on the pinna microarteries,venule diameter,microvascular erythrocyte flow rate in each group(P<0.01).There was significant difference between high-,middle-,low-dose Qizhi Shenan pill group and control group on the pinna microarteries,venule diameter,microvascular erythrocyte flow rate in each group(P<0.05).Conclusion Qizhi Shenan pill can obviously increase the pinna microarteries,venule diameter,microvascular erythrocyte flow rate of mouse with auricle microcirculation disorders model,improve auricle microcirculation of mouse,reduce proteinuria and improve renal function by improving microcirculation and increasing renal blood flow.
引文
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[3]王永钧,张敏鸥.痰瘀互结与肾内微型癥积[J].中国中西医结合肾病杂志,2003,4(1):1-3.
[4]刘洪,郑新.郑新肾病专家阐述芪蛭汤治疗肾病综合征的心得体会[J].中国中西医结合肾病杂志,2010,11(12):1100-1101.
[5]黄海艳,高建东.黄芪药理特性及在糖尿病肾病中运用研究概况[J].实用中医内科杂志,2011,25(10):80-82.
[6]胡波,李敏,牛瑜,等.肾保护性中药的有效成分及作用机制研究[J].中国中西医结合肾病杂志,2006,7(5):305-306.
[7]戈娜,李顺民,孙惠力,等.地龙对糖尿病肾病大鼠肾脏保护作用的研究[J].上海中医药杂志,2010,44(6):103-105.
[8]张洪波,史华,陈波,等.丹参对大鼠肾缺血再灌注损伤保护作用的实验研究[J].中国中医药科技,2002,9(3):148.