3种方法构建BALB/c裸鼠BEL-7402人肝癌模型
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摘要
【目的】以3种不同方法构建BALB/c裸鼠BEL-7402人肝癌模型,并观察BALB/c裸鼠肿瘤生长情况。【方法】将51只BALB/c裸鼠分为A、B、C 3组,每组17只。A组动物皮下注射0.2 m L BEL-7402人肝癌细胞,B组动物于肝脏原位注射0.05 mL BEL-7402人肝癌细胞与Matrigel Matrix的混合物,C组动物于肝脏原位接种BEL-7402人肝癌细胞瘤块组织。从接种后第2周至第5周,每周测定裸鼠体质量,且每组随机取3只动物解剖取肿瘤测量其大小和质量。第5周后,观察记录各组剩余动物(每组5只)的一般状态和存活天数。采用苏木素—伊红(HE)染色观察肿瘤组织病理学变化。【结果】A、B、C 3组实验动物成瘤率均为100%。5周后,A、B、C 3组剩余动物存活天数分别为(90.8±10.2)、(75.6±14.0)、(67.4±11.1) d。解剖所取肿瘤呈结节状,组织病理学检查可见肿瘤细胞呈一定方向排列,细胞呈梭形、多边形,异型明显,可见核分裂。【结论】本实验所采用的3种不同方法均能成功建立BALB/c裸鼠BEL-7402人肝癌模型。
Objective To establish BALB/c nude mouse model bearing human BEL-7402 hepatoma cells by 3 different methods,and to observe the tumor growth in BALB/c nude mouse model. Methods Fifty-one BALB/cnude mice were randomly divided into groups A,B and C,17 mice in each group. Mice of group A were givensubcutaneous injection of 0.2 mL of human BEL-7402 hepatoma cell suspension. Mice of group B were givenintrahepatic injection 0.1 m L of the mixture of human BEL-7402 hepatoma cell suspension and Matrigel Matrix.Mice of group C were given intrahepatic implantation of human BEL-7402 hepatoma cell tumor tissue. From the 2 nd week to 5 thweek after implantation,the body mass of nude mice was recorded weekly,and the size and mass ofthe tumors were measured in three mice randomly sampled from each group. From the 5 thweek, the generalcondition and survival time of the rest model mice(5 mice in each group) were recorded. The pathological featuresof tumor tissues were observed by haematoxylin and eosin(HE) staining method. Results The incidence oftransplanted tumor was 100%. After 5 weeks,the survival time for the remained mice in groups A,B and C was(90.8 ± 10.2),(75.6 ± 14.0),and(67.4 ± 11.1)days,respectively. The appearance of transplanted tumor wasnodular,and the pathological examination results showed that the tumor cells were spindle-like and in polygon,arranging in a certain direction, manifesting as clear heterocysts and nuclear division. Conclusion Theestablishment of BALB/c nude mouse model bearing human BEL-7402 hepatoma cells were successful by 3 different methods.
Objective To establish BALB/c nude mouse model bearing human BEL-7402 hepatoma cells by 3 different methods,and to observe the tumor growth in BALB/c nude mouse model. Methods Fifty-one BALB/cnude mice were randomly divided into groups A,B and C,17 mice in each group. Mice of group A were givensubcutaneous injection of 0.2 mL of human BEL-7402 hepatoma cell suspension. Mice of group B were givenintrahepatic injection 0.1 m L of the mixture of human BEL-7402 hepatoma cell suspension and Matrigel Matrix.Mice of group C were given intrahepatic implantation of human BEL-7402 hepatoma cell tumor tissue. From the 2 nd week to 5 thweek after implantation,the body mass of nude mice was recorded weekly,and the size and mass ofthe tumors were measured in three mice randomly sampled from each group. From the 5 thweek, the generalcondition and survival time of the rest model mice(5 mice in each group) were recorded. The pathological featuresof tumor tissues were observed by haematoxylin and eosin(HE) staining method. Results The incidence oftransplanted tumor was 100%. After 5 weeks,the survival time for the remained mice in groups A,B and C was(90.8 ± 10.2),(75.6 ± 14.0),and(67.4 ± 11.1)days,respectively. The appearance of transplanted tumor wasnodular,and the pathological examination results showed that the tumor cells were spindle-like and in polygon,arranging in a certain direction, manifesting as clear heterocysts and nuclear division. Conclusion Theestablishment of BALB/c nude mouse model bearing human BEL-7402 hepatoma cells were successful by 3 different methods.
引文
[1]叶家才,崔书中,巴明臣.原发性肝癌的流行病学特征及其危险因素[J].实用医学杂志,2008,24(10):1839.
[2]徐加菊,叶欣.兔VX2肿瘤动物模型的建立[J].泰山医学院学报,2010,31(3):227.
[3]胡敏,汪剑峰,赖天幸.肿瘤细胞皮下接种法建立裸鼠肝癌模型的研究[J].今日药学,2016,26(6):397.
[4]沈艳.廖菁,周文江,等.比较两种免疫缺陷动物人肝癌模型的肿瘤生物学特性[J].上海交通大学学报(农业科学版),2009,27(4):335.
[5]张燕.白芍总苷脂质体抗肿瘤活性研究及对荷瘤小鼠免疫功能的影响[D].泸州:泸州医学院,2015.
[6]李雁,汤钊猷.我国肝癌模型研究的历史和现状[J].中华实验外科杂志,2001,18(5):479.
[7]吴孟超.原发性肝癌在中国的治疗和研究现状[J].成都医学院学报,2012,7(2):161.
[8] Schmid M C,Varner J A. Myeloid cell trafficking and tumor angiogenesis[J]. Cancer Lett,2007,250(1):1.
[9]张小艳.裸鼠原位肿瘤移植模型的建立及建模过程中出现过度角质化现象的初步探讨[D].上海:上海交通大学,2015.
[10] Rygaard J. Immunobiology of the mouse mutant “Nude”.Preliminary investigations[J]. Acta Pathol Microbiol Scand,1969,77(4):761.
[11]陈瑞铭,朱德厚,叶秀珍,等.人体肝癌体外细胞株(BEL-7402)的建立及其特征[J].科学通报,1975,20(9):434.
[12]陈瑞铭,朱德厚,叶秀珍,等.人体肝癌体外细胞系BEL-7402的建立及其特征[J].实验生物学报,1978,11(1):37.
[13]农宜熙,黄俊玲,黄赞松,等.人肝癌细胞株的特性及其实验应用[J].世界华人消化杂志,2017,25(2):159.
[2]徐加菊,叶欣.兔VX2肿瘤动物模型的建立[J].泰山医学院学报,2010,31(3):227.
[3]胡敏,汪剑峰,赖天幸.肿瘤细胞皮下接种法建立裸鼠肝癌模型的研究[J].今日药学,2016,26(6):397.
[4]沈艳.廖菁,周文江,等.比较两种免疫缺陷动物人肝癌模型的肿瘤生物学特性[J].上海交通大学学报(农业科学版),2009,27(4):335.
[5]张燕.白芍总苷脂质体抗肿瘤活性研究及对荷瘤小鼠免疫功能的影响[D].泸州:泸州医学院,2015.
[6]李雁,汤钊猷.我国肝癌模型研究的历史和现状[J].中华实验外科杂志,2001,18(5):479.
[7]吴孟超.原发性肝癌在中国的治疗和研究现状[J].成都医学院学报,2012,7(2):161.
[8] Schmid M C,Varner J A. Myeloid cell trafficking and tumor angiogenesis[J]. Cancer Lett,2007,250(1):1.
[9]张小艳.裸鼠原位肿瘤移植模型的建立及建模过程中出现过度角质化现象的初步探讨[D].上海:上海交通大学,2015.
[10] Rygaard J. Immunobiology of the mouse mutant “Nude”.Preliminary investigations[J]. Acta Pathol Microbiol Scand,1969,77(4):761.
[11]陈瑞铭,朱德厚,叶秀珍,等.人体肝癌体外细胞株(BEL-7402)的建立及其特征[J].科学通报,1975,20(9):434.
[12]陈瑞铭,朱德厚,叶秀珍,等.人体肝癌体外细胞系BEL-7402的建立及其特征[J].实验生物学报,1978,11(1):37.
[13]农宜熙,黄俊玲,黄赞松,等.人肝癌细胞株的特性及其实验应用[J].世界华人消化杂志,2017,25(2):159.