HPV16/18 DNA在原发性胃癌患者肿瘤组织中的表达及意义
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摘要
目的探讨人乳头瘤病毒(HPV)16/18 DNA在原发性胃癌患者胃癌组织中的表达情况及其临床意义。方法选取行手术切除且经病理确诊的72例原发性胃癌患者的胃癌组织标本,采用荧光定量聚合酶链反应检测胃癌组织中HPV16/18 DNA的表达情况,分析HPV16/18 DNA表达与原发性胃癌患者临床特征及预后的关系。结果 72例原发性胃癌患者中,HPV16/18 DNA的阳性表达率为56.94%。不同Lauren分型、TNM分期、淋巴结转移情况胃癌患者胃癌组织中HPV16/18 DNA的阳性表达率比较,差异均有统计学意义(P﹤0.05);其中,Lauren分型为弥漫型和肠型、TNM分期为Ⅲ期和Ⅱ期、有淋巴结转移的胃癌患者胃癌组织中HPV16/18 DNA的阳性表达率较高。HPV16/18 DNA阳性表达胃癌患者的5年累积生存率低于HPV16/18 DNA阴性表达胃癌患者,但差异无统计学意义(P﹥0.05)。结论原发性胃癌患者胃癌组织中HPV16/18 DNA的阳性表达率较高,且HPV16/18 DNA表达与胃癌患者的Lauren分型、TNM分期及淋巴结转移情况有关,并可能影响患者的预后。
Objective To analyze the expression and clinical significance of human papillomavirus(HPV) 16/18 DNA in primary gastric cancer tissues. Method A total of 72 cases with pathologically confirmed primary gastric cancer who were treated with surgical resection were included in the study, from whom the specimens were collected and detected by fluorescence quantitative polymerase chain reaction to determine the expression of HPV16/18 DNA. The relationship between the expression of HPV16/18 DNA and clinical features of primary gastric cancer was analyzed. Result The positive expression rate of HPV16/18 DNA in 72 cases primary gastric cancer was 56.94%. The positive expression rates of HPV16/18 DNA in gastric cancer tissues from patients with different Lauren classification, TNM stage, and lymph node metastasis were statistically significant differences(P<0.05); higher positive expression of HPV16/18 DNA was observed in patients with Lauren classification of diffuse type and intestinal type, TNM stage III and II disease, and positive lymph node metastasis. The 5-year accumulate survival rate among patients with positive expression of HPV16/18 DNA was lower compared to those with negative HPV16/18 DNA expression, but the difference was not statistically significant(P>0.05). Conclusion The positive expression rate of HPV16/18 DNA is high in primary gastric cancer tissues,and the positive expression is closely related to Lauren classification, TNM stage and lymph node metastasis, and may affect the patient's prognosis.
Objective To analyze the expression and clinical significance of human papillomavirus(HPV) 16/18 DNA in primary gastric cancer tissues. Method A total of 72 cases with pathologically confirmed primary gastric cancer who were treated with surgical resection were included in the study, from whom the specimens were collected and detected by fluorescence quantitative polymerase chain reaction to determine the expression of HPV16/18 DNA. The relationship between the expression of HPV16/18 DNA and clinical features of primary gastric cancer was analyzed. Result The positive expression rate of HPV16/18 DNA in 72 cases primary gastric cancer was 56.94%. The positive expression rates of HPV16/18 DNA in gastric cancer tissues from patients with different Lauren classification, TNM stage, and lymph node metastasis were statistically significant differences(P<0.05); higher positive expression of HPV16/18 DNA was observed in patients with Lauren classification of diffuse type and intestinal type, TNM stage III and II disease, and positive lymph node metastasis. The 5-year accumulate survival rate among patients with positive expression of HPV16/18 DNA was lower compared to those with negative HPV16/18 DNA expression, but the difference was not statistically significant(P>0.05). Conclusion The positive expression rate of HPV16/18 DNA is high in primary gastric cancer tissues,and the positive expression is closely related to Lauren classification, TNM stage and lymph node metastasis, and may affect the patient's prognosis.
引文
[1]周易,朴正爱,王海,等.胃癌的Lauren分型与ERCC1、BRCA1、XRCC1表达及临床病理特征的关系[J].癌症进展,2016,14(11):1142-1145.
[2]蒋燕明,杨岚,弋文娟.早期宫颈癌前哨淋巴结中高危型HPV16/18 DNA表达的检测及临床意义[J].癌症进展,2016,14(6):597-600.
[3]卢仲明,李奕萱,许咪咪,等.高危型人乳头状瘤病毒16/18在喉癌中的感染及整合状态[J].实用医学杂志,2016,32(9):1422-1424.
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[5]王国荣,韩延霞.高危型人乳头瘤病毒16、18型感染与宫颈癌及癌前病变的相关性分析[J].中国妇幼保健,2018,33(1):67-69.
[6]卫生部.胃癌诊断标准:WS 316-2010[S].北京:中国标准出版社,2010.
[7]郑真,谢聪颖.胃癌的Lauren分型与个体化治疗[J].肿瘤学杂志,2015,21(8):688-693.
[8]刘俊宝,徐珍,郭亚花,等.HPV DNA、HPV E6蛋白联合检测筛查宫颈癌的应用价值[J].中国实验诊断学,2017,21(7):1213-1215.
[9]谢平,白帆,张杰,等.HPV16/18 DNA、Bcl-2、Survivin mRNA在宫颈癌组织中的表达及其临床意义[J].中国临床研究,2015,28(3):293-297.
[10]韦正树,吴明贵,黄林,等.前列腺癌组织中HPV16/18DNA表达变化及其意义[J].山东医药,2016,56(23):83-85.
[11]周菊,游晶,洪志鹏,等.云南省住院肺癌患者HPV16/18感染及p53codon72多态性检测分析[J].中华肿瘤防治杂志,2015,22(1):19-22.
[12]董卫国,于皆平,罗和生,等.人类乳头瘤病毒HPV16,18型感染与胃癌发生的关系[J].世界华人消化杂志,1999,(1):46-48.
[13]Ding GC,Ren JL,Chang FB,et al.Human papillomavirus DNA and P16(INK4A)expression in concurrent esophageal and gastric cardia cancers[J].World J Gastroenterol,2010,16(46):5901-5906.
[14]Zeng Z,Luo FF,Zou LX,et al.Human papillomavirus as a potential risk factor for gastric cancer:a meta-analysis of 1,917 cases[J].Onco Targets Ther,2016,9:7105-7114.
[15]Fakhraei F,Haghshenas MR,Hosseini V,et al.Detection of human papillomavirus DNA in gastric carcinoma specimens in a high-risk region of Iran[J].Biomed Rep,2016,5(3):371-375.
[16]张春林,陈月红,邓泽义,等.中国口腔癌中高危型HPV感染率的Meta分析[J].中华肿瘤防治杂志,2016,23(10):682-689.
[17]Stanley MA,Winder DM,Sterling JC,et al.HPV infection,anal intra-epithelial neoplasia(AIN)and anal cancer:current issues[J].BMC Cancer,2012,12:398.
[18]Hardefeldt HA,Cox MR,Eslick GD.Association between human papillomavirus(HPV)and oesophageal squamous cell carcinoma:a meta-analysis[J].Epidemiol Infect,2014,142(6):1119-1137.
[19]Baandrup L,Thomsen LT,Olesen TB,et al.The prevalence of human papillomavirus in colorectal adenomas and adenocarcinomas:a systematic review and meta-analysis[J].Eur J Cancer,2014,50(8):1446-1461.
[20]马遇庆,蒲红伟,陈朝伦.HPV16/18、p53在新疆贲门腺癌中的表达及其病理生物学意义[J].新疆医科大学学报,2007,29(9):955-957.
[21]王佐君,张亚琼,章月桃.人乳头瘤病毒16幽门螺杆菌细胞毒素相关蛋白A和尿素酶基因A基因型感染与胃癌的相关性分析[J].实用医技杂志,2013,20(10):1061-1064.
[22]徐扬,王玉,王文义.Lauren分型对胃癌术后患者预后的影响[J].中华实用诊断与治疗杂志,2013,27(6):602-603.
[2]蒋燕明,杨岚,弋文娟.早期宫颈癌前哨淋巴结中高危型HPV16/18 DNA表达的检测及临床意义[J].癌症进展,2016,14(6):597-600.
[3]卢仲明,李奕萱,许咪咪,等.高危型人乳头状瘤病毒16/18在喉癌中的感染及整合状态[J].实用医学杂志,2016,32(9):1422-1424.
[4]王霞,李霞斌,李宗恒.HPV16/18在宫颈上皮内瘤变及宫颈癌中表达[J].中国病原生物学杂志,2017,12(2):182-184;188.
[5]王国荣,韩延霞.高危型人乳头瘤病毒16、18型感染与宫颈癌及癌前病变的相关性分析[J].中国妇幼保健,2018,33(1):67-69.
[6]卫生部.胃癌诊断标准:WS 316-2010[S].北京:中国标准出版社,2010.
[7]郑真,谢聪颖.胃癌的Lauren分型与个体化治疗[J].肿瘤学杂志,2015,21(8):688-693.
[8]刘俊宝,徐珍,郭亚花,等.HPV DNA、HPV E6蛋白联合检测筛查宫颈癌的应用价值[J].中国实验诊断学,2017,21(7):1213-1215.
[9]谢平,白帆,张杰,等.HPV16/18 DNA、Bcl-2、Survivin mRNA在宫颈癌组织中的表达及其临床意义[J].中国临床研究,2015,28(3):293-297.
[10]韦正树,吴明贵,黄林,等.前列腺癌组织中HPV16/18DNA表达变化及其意义[J].山东医药,2016,56(23):83-85.
[11]周菊,游晶,洪志鹏,等.云南省住院肺癌患者HPV16/18感染及p53codon72多态性检测分析[J].中华肿瘤防治杂志,2015,22(1):19-22.
[12]董卫国,于皆平,罗和生,等.人类乳头瘤病毒HPV16,18型感染与胃癌发生的关系[J].世界华人消化杂志,1999,(1):46-48.
[13]Ding GC,Ren JL,Chang FB,et al.Human papillomavirus DNA and P16(INK4A)expression in concurrent esophageal and gastric cardia cancers[J].World J Gastroenterol,2010,16(46):5901-5906.
[14]Zeng Z,Luo FF,Zou LX,et al.Human papillomavirus as a potential risk factor for gastric cancer:a meta-analysis of 1,917 cases[J].Onco Targets Ther,2016,9:7105-7114.
[15]Fakhraei F,Haghshenas MR,Hosseini V,et al.Detection of human papillomavirus DNA in gastric carcinoma specimens in a high-risk region of Iran[J].Biomed Rep,2016,5(3):371-375.
[16]张春林,陈月红,邓泽义,等.中国口腔癌中高危型HPV感染率的Meta分析[J].中华肿瘤防治杂志,2016,23(10):682-689.
[17]Stanley MA,Winder DM,Sterling JC,et al.HPV infection,anal intra-epithelial neoplasia(AIN)and anal cancer:current issues[J].BMC Cancer,2012,12:398.
[18]Hardefeldt HA,Cox MR,Eslick GD.Association between human papillomavirus(HPV)and oesophageal squamous cell carcinoma:a meta-analysis[J].Epidemiol Infect,2014,142(6):1119-1137.
[19]Baandrup L,Thomsen LT,Olesen TB,et al.The prevalence of human papillomavirus in colorectal adenomas and adenocarcinomas:a systematic review and meta-analysis[J].Eur J Cancer,2014,50(8):1446-1461.
[20]马遇庆,蒲红伟,陈朝伦.HPV16/18、p53在新疆贲门腺癌中的表达及其病理生物学意义[J].新疆医科大学学报,2007,29(9):955-957.
[21]王佐君,张亚琼,章月桃.人乳头瘤病毒16幽门螺杆菌细胞毒素相关蛋白A和尿素酶基因A基因型感染与胃癌的相关性分析[J].实用医技杂志,2013,20(10):1061-1064.
[22]徐扬,王玉,王文义.Lauren分型对胃癌术后患者预后的影响[J].中华实用诊断与治疗杂志,2013,27(6):602-603.