黄芪的免疫调节作用研究概述
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摘要
黄芪因其广泛的作用范围和明确的功效而成为临床上最常用的中药之一。近年来,国内外学者对黄芪的化学组成及药理活性进行了大量的研究。临床报道及药理实验表明,黄芪及其内含的活性成分具有抗病毒、抗衰老、抗肿瘤等多种生物功能,其中对免疫系统的调节作用受到了广泛的关注。从免疫系统的角度对黄芪及其内含活性物质对机体的固有免疫、适应性免疫、黏膜免疫等的调节作用进行概述,以期从整体上理清黄芪的免疫调节作用脉络,为后续的进一步研究提供理论依据。
Astragali radix is one of the most popular herbs because of its effect range and efficacy.In current years,the scientists from all over the world have been doing great deal of fundamental studies to research the chemical composition and pharmacological activities of astragali radix.Many of the clinical reports and pharmacological experiments claimed that astragali radix and contents contain many biological functions,such as anti-virus,anti-aging and anti-tumor,however,the immune regulation effects of astragali radix is more arrestive.This article would review the immune regulation effects of astragali radix in innate immune,adaptive immunity and mucosal immune.It is hoped that all things about the immune regulation effects of astragali radix would help the next researches.
Astragali radix is one of the most popular herbs because of its effect range and efficacy.In current years,the scientists from all over the world have been doing great deal of fundamental studies to research the chemical composition and pharmacological activities of astragali radix.Many of the clinical reports and pharmacological experiments claimed that astragali radix and contents contain many biological functions,such as anti-virus,anti-aging and anti-tumor,however,the immune regulation effects of astragali radix is more arrestive.This article would review the immune regulation effects of astragali radix in innate immune,adaptive immunity and mucosal immune.It is hoped that all things about the immune regulation effects of astragali radix would help the next researches.
引文
[1]张磊,杨薇,吴瑕,等.不同分子量段黄芪多糖的肠道粘附及对肠道呼吸道防御素分泌的研究[J].中药药理与临床,2012(1):80-83.
[2]黄可儿,李燕舞,王汝俊,等.黄芪总苷对脾虚大鼠壁细胞胃泌素受体作用的研究[J].广州中医药大学学报,2006(1):42-44.
[3]孙雪莲,黄可儿,冯小权,等.黄芪皂苷及黄芪甲苷对慢性胃粘膜损伤大鼠胃粘膜上皮细胞超微结构的影响[J].中药药理与临床,2011(2):75-78.
[4]孙波,陈静,吴娟,等.黄芪多糖对免疫抑制肉鸡生长性能、肠道菌群及免疫功能的影响[J].中国畜牧兽医,2014(8):101-106.
[5]郝钰,邱全瑛,吴珺,等.黄芪多糖促进中性粒细胞与血管内皮细胞黏附及相关黏附分子表达[J].中国中西医结合杂志,2004(5):427-430.
[6]林琳,沈洪,王立新.黄芪甲苷、β-榄香烯增强小鼠巨噬细胞免疫功能的体外实验研究[J].临床检验杂志,2011(2):129-131.
[7]Qin Q,Niu J,Wang Z,et al.Astragalus embranaceus extract activates immune response in macrophages via heparanase[J].Molecules,2012,17(6):7232-7240.
[8]徐荔,何小鹃,柴旺,等.黄芪多糖对内毒素诱导巨噬细胞产生TNF-α、IL-1β及NO的影响[J].中国中医基础医学杂志,2011(5):503-504.
[9]景晓平,裴明,何丽.基于抗体芯片技术的黄芪多糖及板蓝根多糖免疫调节机制研究[J].中华中医药杂志,2013(11):3420-3423.
[10]陈磊,张敏,谢兴亮,等.黄芪组分配伍对小鼠腹腔巨噬细胞免疫活性的影响[J].中国医院药学杂志,2014(10):787-790.
[11]Luo T,Qin J,Liu M,et al.Astragalus polysaccharide attenuates lipopolysaccharide-induced inflammatory responses in microglial cells:regulation of protein kinase B and nuclear factor-kappa B signaling[J].Inflamm Res,2015,64(3-4):205-212.
[12]刘立民,张彦明,陆沭华,等.黄芪多糖促进急性髓细胞白血病患者浆细胞样树突状细胞成熟[J].中国肿瘤生物治疗杂志,2010(6):657-660.
[13]邵鹏,赵鲁杭.黄芪多糖对树突状细胞表型及功能成熟的影响[J].中华微生物学和免疫学杂志,2006,26(7):637-640.
[14]林琳,沈洪,王立新,等.黄芪甲苷、β-榄香烯对小鼠树突状细胞免疫功能的影响[J].东南大学学报(医学版),2011(2):294-297.
[15]王娟,张秋业,陈永兴,等.黄芪对过敏性紫癜患儿急性期外周血树突状细胞分泌细胞因子水平的影响[J].中国中西医结合杂志,2009(9):794-797.
[16]施小山,孙智霞.黄芪注射液对支气管哮喘急性发作的疗效及对细胞免疫功能的影响[J].中国实验方剂学杂志,2012(16):293-296.
[17]季锡清,杜峻峰,陈纲,等.结直肠癌围术期患者免疫功能状态及黄芪注射液的调节作用[J].实用癌症杂志,2011(4):331-334.
[18]刘慰华,张竞之,刘少军,等.黄芪含药血清对NK细胞活性及KLRK1表达的影响[J].现代生物医学进展,2014(24):4637-4640.
[19]崔澂,王润田,胡建军,等.抗肿瘤中药制剂逆转结直肠癌NK细胞免疫抑制的靶分子探讨[J].江苏医药,2011(5):509-512.
[20]吴贤波,杜全宇,梁亚丽,等.黄芪多糖对肺气虚模型小鼠免疫功能的影响[J].中国药房,2012(47):4417-4418.
[21]李萍,景涛.黄芪多糖对大鼠β-防御素基因表达的调节作用[J].中国公共卫生,2004(11):99-100.
[22]Shi L,Yin F,Xin X,et al.Astragalus Polysaccharide Protects Astrocytes from Being Infected by HSV-1 through TLR3/NFkappa B Signaling Pathway[J].Evid Based Complement Alternat Med,2014,2014:285356.
[23]刘印华,赵志强,李树义,等.黄芪多糖对免疫功能影响的体内实验研究[J].河北医药,2015(4):485-487.
[24]杨巧红,苏俊芳,李长福,等.黄芪注射液对诱发性小鼠胸腺萎缩的干预作用及对胸腺淋巴细胞凋亡相关基因Bcl-2的影响[J].中药材,2009(7):1101-1104.
[25]雷澍,朱美飞,江荣林,等.肺炎克雷伯菌肺炎对大鼠胸腺细胞凋亡的影响及其机制[J].中华急诊医学杂志,2011,20(6):627-630.
[26]Abuelsaad A S.Supplementation with Astragalus polysaccharides alters Aeromonas-induced tissue-specific cellular immune response[J].Microb Pathog,2014,66:48-56.
[27]Du X,Zhao B,Li J,et al.Astragalus polysaccharides enhance immune responses of HBV DNA vaccination via promoting the dendritic cell maturation and suppressing Treg frequency in mice[J].Int Immunopharmacol,2012,14(4):463-470.
[28]Zhang A,Zheng Y,Que Z,et al.Astragaloside IV inhibits progression of lung cancer by mediating immune function of Tregs and CTLs by interfering with IDO[J].J Cancer Res Clin Oncol,2014,140(11):1883-1890.
[29]Li Q,Bao JM,Li XL,et al.Inhibiting effect of Astragalus polysaccharides on the functions of CD+4CD25 high Treg cells in the tumor microenvironment of human hepatocellular carcinoma[J].Chin Med J(Engl),2012,125(5):786-793.
[30]Shao BM,Xu W,Dai H,et al.A study on the immune receptors for polysaccharides from the roots of Astragalus membranaceus,a Chinese medicinal herb[J].Biochem Biophys Res Commun,2004,320(4):1103-1111.
[31]张磊,李东晓,吴瑕.玉屏风散总多糖影响肠道黏膜损伤小鼠肠-呼吸道Ig A分泌研究[J].中药药理与临床,2007(5):43-45.
[32]吴瑕,杨薇,张磊,等.不同分子量段黄芪多糖对整体及黏膜免疫功能的影响[J].中国实验方剂学杂志,2011(18):169-172.
[33]刘端勇,赵海梅,周枫,等.黄芪多糖调节小鼠小肠黏膜淋巴细胞因子的表达[J].中国中医基础医学杂志,2008(9):692-693.
[34]张晓莉,宋宝辉,冯艳,等.黄芪及黄芪多糖对隐孢子虫感染小鼠肠黏膜局部免疫的调节作用[J].中国医药科学,2013(3):30-32.
[35]Sun S,Zheng K,Zhao H,et al.Regulatory effect of astragalus polysaccharides on intestinal intraepithelial gammadelta T cells of tumor bearing mice[J].Molecules,2014,19(9):15224-15236.
[2]黄可儿,李燕舞,王汝俊,等.黄芪总苷对脾虚大鼠壁细胞胃泌素受体作用的研究[J].广州中医药大学学报,2006(1):42-44.
[3]孙雪莲,黄可儿,冯小权,等.黄芪皂苷及黄芪甲苷对慢性胃粘膜损伤大鼠胃粘膜上皮细胞超微结构的影响[J].中药药理与临床,2011(2):75-78.
[4]孙波,陈静,吴娟,等.黄芪多糖对免疫抑制肉鸡生长性能、肠道菌群及免疫功能的影响[J].中国畜牧兽医,2014(8):101-106.
[5]郝钰,邱全瑛,吴珺,等.黄芪多糖促进中性粒细胞与血管内皮细胞黏附及相关黏附分子表达[J].中国中西医结合杂志,2004(5):427-430.
[6]林琳,沈洪,王立新.黄芪甲苷、β-榄香烯增强小鼠巨噬细胞免疫功能的体外实验研究[J].临床检验杂志,2011(2):129-131.
[7]Qin Q,Niu J,Wang Z,et al.Astragalus embranaceus extract activates immune response in macrophages via heparanase[J].Molecules,2012,17(6):7232-7240.
[8]徐荔,何小鹃,柴旺,等.黄芪多糖对内毒素诱导巨噬细胞产生TNF-α、IL-1β及NO的影响[J].中国中医基础医学杂志,2011(5):503-504.
[9]景晓平,裴明,何丽.基于抗体芯片技术的黄芪多糖及板蓝根多糖免疫调节机制研究[J].中华中医药杂志,2013(11):3420-3423.
[10]陈磊,张敏,谢兴亮,等.黄芪组分配伍对小鼠腹腔巨噬细胞免疫活性的影响[J].中国医院药学杂志,2014(10):787-790.
[11]Luo T,Qin J,Liu M,et al.Astragalus polysaccharide attenuates lipopolysaccharide-induced inflammatory responses in microglial cells:regulation of protein kinase B and nuclear factor-kappa B signaling[J].Inflamm Res,2015,64(3-4):205-212.
[12]刘立民,张彦明,陆沭华,等.黄芪多糖促进急性髓细胞白血病患者浆细胞样树突状细胞成熟[J].中国肿瘤生物治疗杂志,2010(6):657-660.
[13]邵鹏,赵鲁杭.黄芪多糖对树突状细胞表型及功能成熟的影响[J].中华微生物学和免疫学杂志,2006,26(7):637-640.
[14]林琳,沈洪,王立新,等.黄芪甲苷、β-榄香烯对小鼠树突状细胞免疫功能的影响[J].东南大学学报(医学版),2011(2):294-297.
[15]王娟,张秋业,陈永兴,等.黄芪对过敏性紫癜患儿急性期外周血树突状细胞分泌细胞因子水平的影响[J].中国中西医结合杂志,2009(9):794-797.
[16]施小山,孙智霞.黄芪注射液对支气管哮喘急性发作的疗效及对细胞免疫功能的影响[J].中国实验方剂学杂志,2012(16):293-296.
[17]季锡清,杜峻峰,陈纲,等.结直肠癌围术期患者免疫功能状态及黄芪注射液的调节作用[J].实用癌症杂志,2011(4):331-334.
[18]刘慰华,张竞之,刘少军,等.黄芪含药血清对NK细胞活性及KLRK1表达的影响[J].现代生物医学进展,2014(24):4637-4640.
[19]崔澂,王润田,胡建军,等.抗肿瘤中药制剂逆转结直肠癌NK细胞免疫抑制的靶分子探讨[J].江苏医药,2011(5):509-512.
[20]吴贤波,杜全宇,梁亚丽,等.黄芪多糖对肺气虚模型小鼠免疫功能的影响[J].中国药房,2012(47):4417-4418.
[21]李萍,景涛.黄芪多糖对大鼠β-防御素基因表达的调节作用[J].中国公共卫生,2004(11):99-100.
[22]Shi L,Yin F,Xin X,et al.Astragalus Polysaccharide Protects Astrocytes from Being Infected by HSV-1 through TLR3/NFkappa B Signaling Pathway[J].Evid Based Complement Alternat Med,2014,2014:285356.
[23]刘印华,赵志强,李树义,等.黄芪多糖对免疫功能影响的体内实验研究[J].河北医药,2015(4):485-487.
[24]杨巧红,苏俊芳,李长福,等.黄芪注射液对诱发性小鼠胸腺萎缩的干预作用及对胸腺淋巴细胞凋亡相关基因Bcl-2的影响[J].中药材,2009(7):1101-1104.
[25]雷澍,朱美飞,江荣林,等.肺炎克雷伯菌肺炎对大鼠胸腺细胞凋亡的影响及其机制[J].中华急诊医学杂志,2011,20(6):627-630.
[26]Abuelsaad A S.Supplementation with Astragalus polysaccharides alters Aeromonas-induced tissue-specific cellular immune response[J].Microb Pathog,2014,66:48-56.
[27]Du X,Zhao B,Li J,et al.Astragalus polysaccharides enhance immune responses of HBV DNA vaccination via promoting the dendritic cell maturation and suppressing Treg frequency in mice[J].Int Immunopharmacol,2012,14(4):463-470.
[28]Zhang A,Zheng Y,Que Z,et al.Astragaloside IV inhibits progression of lung cancer by mediating immune function of Tregs and CTLs by interfering with IDO[J].J Cancer Res Clin Oncol,2014,140(11):1883-1890.
[29]Li Q,Bao JM,Li XL,et al.Inhibiting effect of Astragalus polysaccharides on the functions of CD+4CD25 high Treg cells in the tumor microenvironment of human hepatocellular carcinoma[J].Chin Med J(Engl),2012,125(5):786-793.
[30]Shao BM,Xu W,Dai H,et al.A study on the immune receptors for polysaccharides from the roots of Astragalus membranaceus,a Chinese medicinal herb[J].Biochem Biophys Res Commun,2004,320(4):1103-1111.
[31]张磊,李东晓,吴瑕.玉屏风散总多糖影响肠道黏膜损伤小鼠肠-呼吸道Ig A分泌研究[J].中药药理与临床,2007(5):43-45.
[32]吴瑕,杨薇,张磊,等.不同分子量段黄芪多糖对整体及黏膜免疫功能的影响[J].中国实验方剂学杂志,2011(18):169-172.
[33]刘端勇,赵海梅,周枫,等.黄芪多糖调节小鼠小肠黏膜淋巴细胞因子的表达[J].中国中医基础医学杂志,2008(9):692-693.
[34]张晓莉,宋宝辉,冯艳,等.黄芪及黄芪多糖对隐孢子虫感染小鼠肠黏膜局部免疫的调节作用[J].中国医药科学,2013(3):30-32.
[35]Sun S,Zheng K,Zhao H,et al.Regulatory effect of astragalus polysaccharides on intestinal intraepithelial gammadelta T cells of tumor bearing mice[J].Molecules,2014,19(9):15224-15236.