虚寒呕吐模型的复制和吴茱萸汤的止呕作用研究
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摘要
目的:为客观准确评价吴茱萸汤的止呕作用,本研究首次尝试顺铂致呕模型的基础上。方法:采用给予苦寒中药大黄的方式制备了病症结合的虚寒呕吐模型,并基于两种呕吐模型,分别从体质量、生化指标和脏器指数等机体生理生理病理状态,以及异食癖等行为学多个方面对吴茱萸汤的止呕作用进行了评价。结果:大鼠口饲大黄一段时间后表现出了明显的虚寒状态和一定程度的病理指标的变化,而对证给予吴茱萸汤后这种病理状态和病理指标均有较为明显的改善,而不具有虚寒状态的顺铂对照组大鼠给予吴茱萸汤治疗后反而加重了病理指标的变化;吴茱萸汤对两种呕吐模型均有显著的止呕作用(P<0.01),但对大黄加顺铂制备的虚寒呕吐动物模型的作用优于单纯顺铂制备的呕吐模型(P<0.01)。结论:该方法制备的病症结合模型比顺铂致呕模型更适于吴茱萸汤等中医复方治疗虚寒呕吐药效学的评价研究,本研究结果也从以方测证和方证相应的角度反映了该方法复制虚寒呕吐模型的可行性和合理性。
Objective: To objectively and accurately evaluate the antiemetic effect of Wuzhuyi Decoction. In this study we established cisplatin-induced vomitting insufficiency-cold model based on the cisplatin-induced vomitting model for the first time. Methods: Efficacy and rationality of the model were evaluated in terms of body weight,blood biological indices,behaviours and abnormal eating habit. In addition,the antiemetic effect of Wuzhuyu Decoction was also observed. Results: Wuzhuyu Decoction was found possessing antiemetic effect against two models with the cisplatin-induced vomiting insufficiency-cold model more effective than the cisplatin-induced vomiting model. Conclusion: The rhubarb-induced vomitting insufficiency-cold model is more suitable for the evaluation of the effectiveness of antiemetic effects of Chinese compound formulae and this experimental result also supports the feasibility and rationality of the theory "corresponding of compound formulae with syndromes".
Objective: To objectively and accurately evaluate the antiemetic effect of Wuzhuyi Decoction. In this study we established cisplatin-induced vomitting insufficiency-cold model based on the cisplatin-induced vomitting model for the first time. Methods: Efficacy and rationality of the model were evaluated in terms of body weight,blood biological indices,behaviours and abnormal eating habit. In addition,the antiemetic effect of Wuzhuyu Decoction was also observed. Results: Wuzhuyu Decoction was found possessing antiemetic effect against two models with the cisplatin-induced vomiting insufficiency-cold model more effective than the cisplatin-induced vomiting model. Conclusion: The rhubarb-induced vomitting insufficiency-cold model is more suitable for the evaluation of the effectiveness of antiemetic effects of Chinese compound formulae and this experimental result also supports the feasibility and rationality of the theory "corresponding of compound formulae with syndromes".
引文
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[15]隋峰,王汝俊,王建华,等.补脾方药血清对离体脾虚大鼠壁细胞胞内[Ca2+]的影响[J].世界华人消化杂志,13(3):392-394.
[16]隋峰,王汝俊,王建华,等.补中益气汤药物血清对脾虚大鼠离体胃壁细胞胃泌素受体结合容量的影响[J].中药药理与临床,2005,21(2):4-5.
[2]王冰.重广补注黄帝内经素问[M].北京:学苑出版社,2008.
[3]张婷.方剂辨证论治方法体系之建立—吴茱萸汤证的辨证施治[D].哈尔滨:黑龙江中医药大学,2015.
[4]郑攀.吴茱萸汤证机探析[J].时珍国医国药,2006,17(10):1928-1929.
[5]唐映红,窦昌贵.吴茱萸汤温脾止泻作用的实验研究[J].中药药理与临床,1990,6(1):6-9.
[6]潘守杰,殷常春,丰育来.吴茱萸汤治疗晚期胃癌呕吐32例临床观察[J].辽宁中医杂志,2009,36(9):1519-1520.
[7]张雪,邹大威.通窍活血汤合方治疗偏头痛[J].辽宁中医杂志,2014,41(12):2694-2696.
[8]杨枫,罗红斌,王霞.加减吴茱萸汤治疗经行头痛34例[J].光明中医,2009,24(8):1485-1486.
[9]Ji C X,Fan D S,Li W,et al.Evaluation of the anti-ulcergenicactivity of the antidepressants duloxetine,amitriptyline,fluoxetine and mirtazapine in different models of experimental gastric ulcer in rats[J].Eur J Pharm,2012,691(1-3):46.
[10]郭丽霞,郭玉兰.舒适护理减缓食管癌术后化疗呕吐的效果观察[J].护理研究,2012,26(6):539-540.
[11]曾麟,杨水秀,袁海珍.吴茱萸对化疗呕吐抑制的临床观察及其机制研究.中国当代医药,2016,23(8):58-60
[12]Craig J B,Powell B L.The management of nausea and vomiting in clinical oncology[J].American Journal of the Medical Sciences,1987,293(1):34-44.
[13]于功昌,张勇,杜秀伟,等.小半夏汤对化疗性异食癖大鼠P物质和NK1受体的影响[J].中药药理与临床,2015,31(1):17-20
[14]杨志宏,赵德雪,侯海燕.辣椒提取物对3种异嗜高岭土模型大鼠的止呕作用[J].中国新药杂志,2007,6(20):1682-1685.
[15]隋峰,王汝俊,王建华,等.补脾方药血清对离体脾虚大鼠壁细胞胞内[Ca2+]的影响[J].世界华人消化杂志,13(3):392-394.
[16]隋峰,王汝俊,王建华,等.补中益气汤药物血清对脾虚大鼠离体胃壁细胞胃泌素受体结合容量的影响[J].中药药理与临床,2005,21(2):4-5.