补中益气丸对脾虚大鼠能量物质及AMPK的影响
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摘要
目的:从ATP、AMP、AMPK的变化探讨脾虚证与能量代谢的关系。方法:采用高效液相色谱法检测大黄脾虚大鼠ATP、AMP的变化,荧光定量PCR方法检测AMPK-α基因的表达。结果:脾虚模型血浆、肝组织AMP/ATP均增高;肌肉中AMPK-α基因表达显著降低,肝组织中AMPK-α基因表达显著升高;补中益气丸对上述模型中异常的能量物质有调节作用。结论:脾虚静息状态下ATP、AMP及AMPK在不同组织中表达不同,肝组织能量需求大于肌肉组织,补中益气丸可以调整脾虚状态下紊乱的能量物质。
Objective: To discuss the relationship of spleen deficiency syndrome and energy metabolism based on the changes of ATP,AMP and AMPK. Methods: Spleen deficiency syndrome rat model was made by intragastric administration of Rheum. ATP and AMP in plasma,muscle and liver were detected by HPLC. AMPK-α expression in muscle and liver were detected by RT-PCR. Results: Compared with normal group,AMP / ATP ratio were increased obviously in the plasma and liver of model group. AMPK-α gene expression in muscle of model decreased obviously but increased in liver. Buzhong Yiqi pill could regulate the abnormal energy substance above in the model group. Conclusions: There are some differences of ATP,AMP and AMPK in different tissues,and energy supply in liver could be more than in muscle in the spleen deficiency syndrome. Buzhong Yiqi pill could regulate the disordered energy substances in the spleen deficiency syndrome.
Objective: To discuss the relationship of spleen deficiency syndrome and energy metabolism based on the changes of ATP,AMP and AMPK. Methods: Spleen deficiency syndrome rat model was made by intragastric administration of Rheum. ATP and AMP in plasma,muscle and liver were detected by HPLC. AMPK-α expression in muscle and liver were detected by RT-PCR. Results: Compared with normal group,AMP / ATP ratio were increased obviously in the plasma and liver of model group. AMPK-α gene expression in muscle of model decreased obviously but increased in liver. Buzhong Yiqi pill could regulate the abnormal energy substance above in the model group. Conclusions: There are some differences of ATP,AMP and AMPK in different tissues,and energy supply in liver could be more than in muscle in the spleen deficiency syndrome. Buzhong Yiqi pill could regulate the disordered energy substances in the spleen deficiency syndrome.
引文
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[5]冯起国,邓国伟.艾灸对脾虚大鼠骨骼肌细胞线粒体酶活性的影响[J].辽宁中医杂志,2000,27(7):315-317.
[6]曾益宏,刘友章,徐升.益气健脾法对脾虚证大鼠模型骨骼肌线粒体ATPase活性的影响[J].长春中医药大学学报,2009,25(2):171-172.
[7]樊新荣,朱文锋,伍参荣,等.太阴与阳明病证实验大鼠小肠D-木糖的吸收功能及肝脏ATP酶活性研究[J].上海中医药大学学报,2007,21(4):47-49.
[8]彭艳,易受乡,林亚平,等.艾灸对脾虚大鼠空肠组织ATP含量和ATP酶活性的影响[J].中国现代医学杂志,2013,23(1):8-13.
[9]Stapleton D,Mitchelhill KI,Gao G,et al.Mammalian AMPactivated protein kinase subfamily[J].J Biol Chem,1996,271(2):611-614.
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[11]宋雅芳,王汝俊,刘友章,等.健脾益气中药对脾虚大鼠肝组织线粒体功能的影响[J].中药新药与临床药理,2009,20(5):423-426.
[12]刘友章,王昌俊,周俊亮,等.四君子汤修复脾虚大鼠线粒体细胞色素氧化酶的作用及机制[J].中国临床康复,2006,10(35):118-122.
[2]Hardie DG.AMP-activated protein kinase:An energy sensor that regulates all aspects of cell function[J].Genes&Dev,2011,25(18):1895-1908.
[3]李燕舞,王汝俊,王建华,等.补脾方药对脾虚大鼠壁细胞胃泌素受体及细胞内钙离子浓度的影响[J].中医杂志,2006,47(8):616-618.
[4]米红,李燕舞,王晓燕,等.黄芪总苷对脾虚大鼠胃黏膜保护机制探讨[J].中药药理与临床,2012,28(5):61-63.
[5]冯起国,邓国伟.艾灸对脾虚大鼠骨骼肌细胞线粒体酶活性的影响[J].辽宁中医杂志,2000,27(7):315-317.
[6]曾益宏,刘友章,徐升.益气健脾法对脾虚证大鼠模型骨骼肌线粒体ATPase活性的影响[J].长春中医药大学学报,2009,25(2):171-172.
[7]樊新荣,朱文锋,伍参荣,等.太阴与阳明病证实验大鼠小肠D-木糖的吸收功能及肝脏ATP酶活性研究[J].上海中医药大学学报,2007,21(4):47-49.
[8]彭艳,易受乡,林亚平,等.艾灸对脾虚大鼠空肠组织ATP含量和ATP酶活性的影响[J].中国现代医学杂志,2013,23(1):8-13.
[9]Stapleton D,Mitchelhill KI,Gao G,et al.Mammalian AMPactivated protein kinase subfamily[J].J Biol Chem,1996,271(2):611-614.
[10]Stein SC,Woods A,Jones NA,et al.The regulation of AMPactivated protein kinase by phosphorylation[J].Biochem J2000,345(Pt 3):437-443.
[11]宋雅芳,王汝俊,刘友章,等.健脾益气中药对脾虚大鼠肝组织线粒体功能的影响[J].中药新药与临床药理,2009,20(5):423-426.
[12]刘友章,王昌俊,周俊亮,等.四君子汤修复脾虚大鼠线粒体细胞色素氧化酶的作用及机制[J].中国临床康复,2006,10(35):118-122.