益气养阴健脾法对小鼠胃肠动力影响的实验研究
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摘要
目的考察以益气养阴健脾法为指导组成的方剂对正常及免疫抑制模型小鼠胃肠动力的影响。方法采用营养性半固体糊加活性炭法测定胃内容物残留率;墨水推进法测定小肠推进率;酶联免疫吸附法测定血浆胃动素(MTL)。用环磷酰胺经腹腔注射制备免疫抑制模型。结果益气养阴健脾法组方药物中、高剂量组对正常小鼠胃排空率,小肠推进率和胃肠激素水平的作用与空白对照组相比均有显著性差异。免疫抑制状态下低、中、高剂量组的小鼠小肠推进率与模型组相比均有显著性差异,中、高剂量组的小鼠胃排空率和胃肠激素水平与模型组相比均有显著性差异。结论以益气养阴健脾法指导组方的药物对正常小鼠及免疫抑制模型小鼠胃肠动力及胃肠激素水平均有促进作用。
Objective The effect of prescriptions on gastrointestinal motility in normal and immunosuppressed mice was investigated,which were taken as Reinforcing Qi and Nourishing Yin for Spleen method. Methods The residual contents of stomach were determined by the method of nutritional semi solid paste plus activated carbon. Small intestinal propulsion rates were measured by the method of ink propulsion. Plasma motilin level(MTL) was measured by enzyme linked immunosorbent assay. The immunosuppressive model herein was prepared by intraperitoneal injection of cyclophosphamide. Results The efficacy of the prescription Reinforcing Qi and Nourishing Yin for Spleen Method was represented by the medium dose group and the high dose group on the following factors. The gastric emptying rate,the small intestinal propulsion rate and the level of gastrointestinal hormones in normal mice were compared with the blank control group and there were significant differences. The intestinal propulsive rates of mice in low,medium and high dose groups were significantly different from those in model group under immunosuppressive condition. The gastric emptying rate and gastrointestinal hormone levels in the middle and high dose groups were significantly different from those in the model group. Conclusion The effect of prescription on gastrointestinal motility and gastrointestinal hormone levels in normal mice and immunosuppressive mice was promoted,the prescription of which was guided as Reinforcing Qi and Nourishing Yin for Spleen method.
Objective The effect of prescriptions on gastrointestinal motility in normal and immunosuppressed mice was investigated,which were taken as Reinforcing Qi and Nourishing Yin for Spleen method. Methods The residual contents of stomach were determined by the method of nutritional semi solid paste plus activated carbon. Small intestinal propulsion rates were measured by the method of ink propulsion. Plasma motilin level(MTL) was measured by enzyme linked immunosorbent assay. The immunosuppressive model herein was prepared by intraperitoneal injection of cyclophosphamide. Results The efficacy of the prescription Reinforcing Qi and Nourishing Yin for Spleen Method was represented by the medium dose group and the high dose group on the following factors. The gastric emptying rate,the small intestinal propulsion rate and the level of gastrointestinal hormones in normal mice were compared with the blank control group and there were significant differences. The intestinal propulsive rates of mice in low,medium and high dose groups were significantly different from those in model group under immunosuppressive condition. The gastric emptying rate and gastrointestinal hormone levels in the middle and high dose groups were significantly different from those in the model group. Conclusion The effect of prescription on gastrointestinal motility and gastrointestinal hormone levels in normal mice and immunosuppressive mice was promoted,the prescription of which was guided as Reinforcing Qi and Nourishing Yin for Spleen method.
引文
[1]王雨秾,陈德兴.中药对胃肠动力实验研究的影响[J].辽宁中医学院学报,2005,7(2):117.
[2]魏睦新,邹百仓.胃肠动力中药的调节机理研究进展[J].浙江中西医结合杂志,2002,12(7):456.
[3]李慧臻.李永成教授应用中药促胃肠动力的经验总结[J].天津中医药,2006,23(2):101.
[4]Siegel R,Naishadham D,Jemal A.Cancer statistics,2012[J].CA Cancer J Clin,2012,62(1):10.
[5]Tian J,Chen ZC,Wu B,et al.Comparison of quality of life between urban and rural gastric cancerpatients and analysis of influencing factors[J].World J Gastroenterol,2004,10(20):2940.
[6]张钊,郝迎旭,李佩文,等.中医药防治研究放化疗毒副反应40年[J].中国自然医学杂志,2000,2(2):114.
[7]中华人民共和国卫生部.保健食品检验与评价技术规范食品安全性毒理学评价程序和方法(2003版)[S].北京:中国标准出版社,2003.
[8]陆英杰,连至诚.胃肠激素对胃肠动力的影响[J].免疫学杂志,2006,22(3):94.
[9]郭金秀,刘孟安,王慧凯.中药胃肠舒片对胃肠动力障碍小鼠胃排空及肠推进影响的实验研究[J].辽宁中医杂志,2010,37(4):751.
[10]李叶,罗云坚,黄穗平.功能性消化不良与胃肠动力和胃肠激素关系的研究进展[J].新中医,2003,35(2):74.
[11]张卜瑗,仇娜.十全大补汤减轻化疗骨髓抑制的临床观察[J].现代中西医结合杂志,2009,18(18):2157.
[2]魏睦新,邹百仓.胃肠动力中药的调节机理研究进展[J].浙江中西医结合杂志,2002,12(7):456.
[3]李慧臻.李永成教授应用中药促胃肠动力的经验总结[J].天津中医药,2006,23(2):101.
[4]Siegel R,Naishadham D,Jemal A.Cancer statistics,2012[J].CA Cancer J Clin,2012,62(1):10.
[5]Tian J,Chen ZC,Wu B,et al.Comparison of quality of life between urban and rural gastric cancerpatients and analysis of influencing factors[J].World J Gastroenterol,2004,10(20):2940.
[6]张钊,郝迎旭,李佩文,等.中医药防治研究放化疗毒副反应40年[J].中国自然医学杂志,2000,2(2):114.
[7]中华人民共和国卫生部.保健食品检验与评价技术规范食品安全性毒理学评价程序和方法(2003版)[S].北京:中国标准出版社,2003.
[8]陆英杰,连至诚.胃肠激素对胃肠动力的影响[J].免疫学杂志,2006,22(3):94.
[9]郭金秀,刘孟安,王慧凯.中药胃肠舒片对胃肠动力障碍小鼠胃排空及肠推进影响的实验研究[J].辽宁中医杂志,2010,37(4):751.
[10]李叶,罗云坚,黄穗平.功能性消化不良与胃肠动力和胃肠激素关系的研究进展[J].新中医,2003,35(2):74.
[11]张卜瑗,仇娜.十全大补汤减轻化疗骨髓抑制的临床观察[J].现代中西医结合杂志,2009,18(18):2157.