HPLC法测定盆炎净洗剂中芍药苷的含量
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摘要
目的建立盆炎净洗剂中芍药苷的含量测定方法。方法采用C18柱(4.6mm×250mm,5μm);流动相为乙腈-0.1%磷酸溶液(14∶86);检测波长230nm;流速1.0mL·min-1。结果芍药苷在80.442ng~1608.840ng的范围内线性关系良好(r=0.9993)范围内与峰面积线性关系良好,平均回收率为95.64±1.17(RSD1.22%)。结论该方法简单易行、准确可靠,可作为盆炎净洗剂的质量控制方法。
OBJECTIVE To establish the method for determination of paeoniflorin in Penyanjing Lotion by HPLC. METHODS C18column( 4. 6mm × 250mm,5μm) was used with mobile phase of Acetonitrile-0. 1% H3PO4( 14∶86). The detection wavelength was 230nm,and the flow rate was 1. 0mL·min- 1. RESULTS The linear relationship of paeoniflorin was in the range of 80. 442ng ~ 1608. 840ng( r = 0. 9993). The average recover was 95. 64 ± 1. 17( RSD1. 22%). CONCLUSION The method is simple,accurate,reliable,and can be used for quality control of Penyanjing Lotion.
OBJECTIVE To establish the method for determination of paeoniflorin in Penyanjing Lotion by HPLC. METHODS C18column( 4. 6mm × 250mm,5μm) was used with mobile phase of Acetonitrile-0. 1% H3PO4( 14∶86). The detection wavelength was 230nm,and the flow rate was 1. 0mL·min- 1. RESULTS The linear relationship of paeoniflorin was in the range of 80. 442ng ~ 1608. 840ng( r = 0. 9993). The average recover was 95. 64 ± 1. 17( RSD1. 22%). CONCLUSION The method is simple,accurate,reliable,and can be used for quality control of Penyanjing Lotion.
引文
[1]邓松岳,王伟丽,朱明伟,等.HPLC法测定桂丹莪棱口服液中芍药苷的含量[J].中国医药导报,2012,9(10):139-140.
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[2]张颖,王伟丽,朱明伟.HPLC法测定胃舒康片中芍药苷含量[J].中国药业,2012,21(23):15-16.
[3]湛延风,严建业,罗堃.高效液相色谱法测定妇炎康胶囊中芍药苷的含量[J].中南药学,2006,4(2):36-38.
[4]李海燕,康建.冠心生脉口服液质量标准研究[J].中国药业,2011,20(1):18-20.
[5]胥爱丽,庄玉坚.胃舒口服液的质量标准研究[J].中国实验方剂学杂志,2008,14(6):9-11.