彝良小草坝乌天麻对东莨菪碱所致记忆障碍模型小鼠的影响及机制研究
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摘要
目的研究彝良小草坝乌天麻提取物对东莨菪碱所致记忆障碍模型小鼠的影响,为阐明天麻道地性及临床应用价值提供实验依据。方法采用腹腔注射东莨菪碱建立小鼠记忆障碍模型,将ICR小鼠随机分为6组:空白组、模型组、石杉碱甲组、小草坝乌天麻水提物低、中、高剂量组。通过Morris水迷宫实验观察彝良小草坝乌天麻对小鼠记忆的影响差异,并测定海马、皮层组织的乙酰胆碱酯酶(acetylcholinesterase,AchE)、超氧化物歧化酶(superoxide dismutase,SOD)、丙二醛(malondialdehyde,MDA)的活性。结果小草坝天麻水提物能缩短东莨菪碱所致记忆获得障碍模型小鼠的逃避潜伏期及搜索距离(P<0.05),明显增加目标象限时间百分比(P<0.01),提高小鼠脑组织中的乙酰胆碱含量(P<0.01)和SOD活力(P<0.05),降低小鼠脑组织中的MDA含量(P<0.01)。结论小草坝天麻水提物能明显改善模型小鼠的记忆获得能力,其作用与增强胆碱能神经系统功能及增强抗氧化能力有关。
Aim To study the effects of the extract of Gastrodia elata Bl. from Yiliang on mice with memory impairment induced by scopolamine and to provide experimental basis for clarifying the genuineness and the clinical value of Gastrodia elata Bl..Methods The method of intraperitoneal injection of scopolamine was used to establish a mouse model of memory impairment. ICR mice were randomly divided into six groups: blank control group, model group, positive drug group, Gastrodia elata Bl low dose group, middle dose group, and high dose group. Morris water maze was used to examine the influences of Gastrodia elata Bl. from Yiliang on the memory, and the levels of AchE, SOD and MDA in the hippocampus and cortex were determined as well.Results The total extract of Gastrodia elata Bl. from Yiliang could shorten the escape latency and distance in target quadrant of scopolamine-induced memory impairment model mice(P<0.05), significantly increase the original quadrant time percentage(P<0.01), increase acetylcholine content(P<0.01) and SOD activity(P<0.05) in brain tissues of mice, and decrease MDA content(P<0.01) in brain tissues of mice.Conclusions The total extract of Gastrodia elata Bl. from Yiliang can obviously improve the memory acquisition ability of the model mice, which is related to the enhancement of the function of the cholinergic nervous system and thus the antioxidant capacity.
Aim To study the effects of the extract of Gastrodia elata Bl. from Yiliang on mice with memory impairment induced by scopolamine and to provide experimental basis for clarifying the genuineness and the clinical value of Gastrodia elata Bl..Methods The method of intraperitoneal injection of scopolamine was used to establish a mouse model of memory impairment. ICR mice were randomly divided into six groups: blank control group, model group, positive drug group, Gastrodia elata Bl low dose group, middle dose group, and high dose group. Morris water maze was used to examine the influences of Gastrodia elata Bl. from Yiliang on the memory, and the levels of AchE, SOD and MDA in the hippocampus and cortex were determined as well.Results The total extract of Gastrodia elata Bl. from Yiliang could shorten the escape latency and distance in target quadrant of scopolamine-induced memory impairment model mice(P<0.05), significantly increase the original quadrant time percentage(P<0.01), increase acetylcholine content(P<0.01) and SOD activity(P<0.05) in brain tissues of mice, and decrease MDA content(P<0.01) in brain tissues of mice.Conclusions The total extract of Gastrodia elata Bl. from Yiliang can obviously improve the memory acquisition ability of the model mice, which is related to the enhancement of the function of the cholinergic nervous system and thus the antioxidant capacity.
引文
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[12] 刘智慧, 马 浩, 王伟平,等. 天麻素及派立辛改善东莨菪碱致学习记忆障碍的构效关系[J]. 药学学报, 2016,51(5):743-8.[12] Liu Z H, Ma H, Wang W P,et al. Structure-activity relationship of gastrodin and parishins on learning and memory deficits induced by scopolamine[J].Acta Pharm Sin, 2016,51(5):743-8.
[2] 韩春妮, 何芳雁, 田 野,等. 天麻提取物对记忆获得障碍模型小鼠胆碱能系统的影响[J]. 中国药业, 2014,23(21):3-4.[2] Han C N,He F Y,Tian Y,et al. Effects of extract from Gastrodiae Rhizoma on cholinergic system in mouse memory acquisition barrier model[J].Chin Pharm,2014,23(21):3-4.
[3] 王忠巧,贺 娜,徐 田.小草坝天麻仿野生栽培技术[J].林业建设,2015,10(5):63-6.[3] Wang Z Q,He N,Xu T. Bionic cultivation techniques of gastrodia elata in Xiaocaoba[J].Forestry Construction,2015,10(5):63-6.
[4] 高 莉, 彭晓明, 张富春,等. 不同剂量东莨菪碱对小鼠学习记忆功能的影响[J]. 医药导报, 2013, 32(5):573-6.[4] Gao L,Peng X M,Zhang F C,et al. Effects of Scopolamine at different doses on learning and memory function in mice[J].Herald Med,2013, 32(5):573-6.
[5] Blake M G, Boccia M M, Krawczyk M C, et al. Choline reverses scopolamine-induced memory impairment by improving memory reconsolidation[J]. Neurobiol Learn Mem, 2012, 98(2):112-21.
[6] Blake M G, Boccia M M, Baratti C M. Behavioral differences on memory retrieval between two variants of step-through inhibitory avoidance task in mice[J]. Neurosci Lett, 2008, 444(1):102-5.
[7] 宋 奇,王 琴,吴韦黎,等.新药T-006对东莨菪碱所致记忆障碍模型动物学习记忆的改善作用[J].中国药理学通报,2016,32(6):812-7.[7] Song Q, Wang Q, Wu W L, et al. Effects of new drug T-006 on improving learning and memory abilities in scopolamine-induced dementia Kunming mice[J].Chin Pharmacol Bull,2016,32(6):812-7.
[8] 周慧君. 天麻有效成分的药理作用与临床应用研究进展[J]. 中医临床研究, 2016, 8(22):56-8.[8] Zhou H J.A review on pharmacological action of active principle in gastrodia elata and the clinical application[J].Clin J Chin Med,2016, 8(22):56-8.
[9] Chen W, Cheng X, Chen J, et al. Lycium barbarum polysaccharides prevent memory and neurogenesis impairments in scopolamine-treated rats[J]. PLoS One, 2014, 9(2):e88076.
[10] Chen Q, Prior M, Dargusch R, et al. A novel neurotrophic drug for cognitive enhancement and Alzheimer′s disease [J]. PLoS One,2011,6(12):e27865.
[11] 明 建, 曾凯芳, 吴素蕊,等. 天麻多糖PGEB-3-H对东莨菪碱所致小鼠学习记忆障碍的影响[J]. 食品科学, 2010, 31(3):246-9.[11] Ming J, Zeng K F, Wu S R, et al. Effect of soluble polysaccharide PGEB-3-H from Gastrodia elata Blume on scopolamine-induced learning and memory disorders in mice[J].Food Sci,2010, 31(3):246-9.
[12] 刘智慧, 马 浩, 王伟平,等. 天麻素及派立辛改善东莨菪碱致学习记忆障碍的构效关系[J]. 药学学报, 2016,51(5):743-8.[12] Liu Z H, Ma H, Wang W P,et al. Structure-activity relationship of gastrodin and parishins on learning and memory deficits induced by scopolamine[J].Acta Pharm Sin, 2016,51(5):743-8.