江苏小麦品种春化和光周期基因的组成分析
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摘要
利用春化基因Vrn-A1、Vrn-B1、Vrn-D1、Vrn-B3及光周期基因Ppd-A1、Ppd-B1和Ppd-D1的STS分子标记对选自江苏境内的114份小麦品种(系)进行检测,研究江苏小麦品种中春化和光周期基因的分布情况。结果表明:春化基因显性等位变异的分布频率依次为Vrn-D1a(57.9%)、Vrn-D1b(15.8%)、Vrn-A1b(5.3%)、Vrn-B1 (2.6%)和Vrn-B3 (0)。江苏淮南麦区中Vrn-D1a的分布频率最高(77.5%);江苏淮北麦区中Vrn-D1b的分布频率最高(38.2%)。江苏地区共存在6种春化基因等位变异组合:vrn-A1+vrn-B1+Vrn-D1a(56.1%)、vrn-A1+vrn-B1+vrn-D1 (21.1%)、vrn-A1+vrn-B1+Vrn-D1b(15.8%)、Vrn-A1b+vrn-B1+vrn-D1 (2.6%)、vrn-A1+Vrn-B1+vrn-D1 (2.6%)和Vrn-A1b+vrn-B1+Vrn-D1a(1.8%)。淮南麦区vrn-A1+vrn-B1+Vrn-D1a(75.0%)组合占主导地位;淮北麦区以vrn-A1+vrn-B1+vrn-D1 (50.0%)和vrn-A1+vrn-B1+Vrn-D1b(38.2%)组合为主。所有品种(系)均含有光周期敏感型基因Ppd-A1b和Ppd-B1b,有7个品种携带光周期敏感型基因Ppd-D1b,其余品种均携带光周期不敏感型基因Ppd-D1a,占93.9%。
In order to elucidate the distribution of vernalization and photoperiod genes in wheat varieties from Jiangsu region, 114 wheat varieties(lines) from Jiangsu province were characterized by STS markers for vernalization and photoperiod genesloci Vrn-A1, Vrn-B1, Vrn-D1, Vrn-B3, Ppd-A1, Ppd-B1 and Ppd-D1. The results showed that the distribution frequencies of dominant alleles of vernalization genes were Vrn-D1a(57.9%), Vrn-D1b(15.8%), Vrn-A1b(5.3%), Vrn-B1(2.6%) and Vrn-B3(0). The distribution frequency of Vrn-D1a was the highest in Huainan wheat region of Jiangsu province, reaching 77.5%, and that of Vrn-D1b was the highest in Huaibei wheat region of Jiangsu province, reaching 38.2%. Six vernalization gene allele variant combinations were found in Jiangsu region: vrn-A1+vrn-B1+Vrn-D1a(56.1%), vrn-A1+vrn-B1+vrn-D1(21.1%), vrn-A1+vrn-B1+Vrn-D1b(15.8%), Vrn-A1b+Vrn-B1+vrn-D1(2.6%), vrn-A1+Vrn-B1+vrn-D1(2.6%) and Vrn-A1b+vrn-B1+Vrn-D1a(1.8%). The combination of vrn-A1+vrn-B1+Vrn-D1a(75.0%) was dominant in Huainan wheat region, while the combination of vrn-A1+vrn-B1+vrn-D1(50.0%) and vrn-A1+vrn-B1+Vrn-D1b(38.2%) were dominant in Huaibei wheat region. All varieties(lines) contained photoperiod-sensitive genes Ppd-A1 b and Ppd-B1 b;seven cultivars carried photoperiod-sensitive genes PpdD1 b,while the rest carried photoperiod-insensitive genes Ppd-D1 a,accounting for 93.9%.
In order to elucidate the distribution of vernalization and photoperiod genes in wheat varieties from Jiangsu region, 114 wheat varieties(lines) from Jiangsu province were characterized by STS markers for vernalization and photoperiod genesloci Vrn-A1, Vrn-B1, Vrn-D1, Vrn-B3, Ppd-A1, Ppd-B1 and Ppd-D1. The results showed that the distribution frequencies of dominant alleles of vernalization genes were Vrn-D1a(57.9%), Vrn-D1b(15.8%), Vrn-A1b(5.3%), Vrn-B1(2.6%) and Vrn-B3(0). The distribution frequency of Vrn-D1a was the highest in Huainan wheat region of Jiangsu province, reaching 77.5%, and that of Vrn-D1b was the highest in Huaibei wheat region of Jiangsu province, reaching 38.2%. Six vernalization gene allele variant combinations were found in Jiangsu region: vrn-A1+vrn-B1+Vrn-D1a(56.1%), vrn-A1+vrn-B1+vrn-D1(21.1%), vrn-A1+vrn-B1+Vrn-D1b(15.8%), Vrn-A1b+Vrn-B1+vrn-D1(2.6%), vrn-A1+Vrn-B1+vrn-D1(2.6%) and Vrn-A1b+vrn-B1+Vrn-D1a(1.8%). The combination of vrn-A1+vrn-B1+Vrn-D1a(75.0%) was dominant in Huainan wheat region, while the combination of vrn-A1+vrn-B1+vrn-D1(50.0%) and vrn-A1+vrn-B1+Vrn-D1b(38.2%) were dominant in Huaibei wheat region. All varieties(lines) contained photoperiod-sensitive genes Ppd-A1 b and Ppd-B1 b;seven cultivars carried photoperiod-sensitive genes PpdD1 b,while the rest carried photoperiod-insensitive genes Ppd-D1 a,accounting for 93.9%.
引文
[1] 尹钧.小麦温光发育研究进展(Ⅰ):春化和光周期发育规律 [J].麦类作物学报,2016,36(6):681-688.
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[3] PUGSLEY A T.A genetic analysis of the spring-winter habit of growth in wheat [J].Australian Journal of Agricultural Research,1971,22(1):21-31.
[4] YAN L,LOUKOIANOV A,BLECHL A,et al.The wheat VRN2 gene is a flowering repressor down-regulated byvernalization [J].Science,2004,303(5664):1640-1644.
[5] DISTELFELD A,TRANQUILLI G,Li C,et al.Genetic and molecular characterization of the VRN2 loci in tetraploid wheat [J].Plant Physiology,2009,149(1):245-257.
[6] YAN L,FU D,LI C,et al.The wheat and barley vernalization gene VRN3 is an orthologue of FT [J].Proceedings of the National Academy of Sciences of the United States of America,2006,103(51):19581-19586.
[7] YOSHIDA T,NISHIDA H,ZHU J,et al.Vrn-D4 is avernalization gene located on the centromeric region of chromosome 5D in hexaploid wheat [J].Theoretical & Applied Genetics,2010,120(3):543-552.
[8] YAN L,HELGUERA M,KATO K,et al.Allelic variation at the VRN-1,promoter region in polyploid wheat [J].Theor Appl Genet,2004,109(8):1677-1686.
[9] YAN L,LOUKOIANOY A,TRANQUILLI G,et al.Positional cloning of the wheat vernalization gene VRN1 [J].Proceedings of the National Academy of Sciences of the United States of America,2003,100(10):6263-6268.
[10] FU D,SZUCS P,YAN L,et al.Large deletions within the first intron in VRN-1 are associated with spring growth habit in barley and wheat [J].Molecular Genetics & Genomics,2005,274(4):442-443.
[11] 姜莹,黄林周,胡银岗.中国小麦地方品种春化基因的分布及其与冬春性的关系 [J].中国农业科学,2010,43(13):2619-2632.
[12] ZHANG J,WANG Y,WU S,et al.A single nucleotide polymorphism at the Vrn-D1 promoter region in common wheat is associated with vernalization response [J].Theoretical Applied Genetics,2012,125(8):1697-1704.
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[14] 王敏慧.小麦春化基因Vrn-1新等位变异发掘 [D].北京:中国农业科学院,2011.
[15] 潘瑞炽.植物生理学 [M].北京:高等教育出版社,2008:276.
[16] WELSH J R,KEIN D L,PIRASTEH B,et al.Genetic control of photoperiod response in wheat [C]// Missouri:Proceeding of the 4th International wheat Genetic Symposium.1973:89
[17] WORLAND A J,BORNER A,KORZUN V,et al.The influence of photoperiod genes on the adaptability of European winter wheats [J].Euphytica,1998,100(1/2/3):385-394.
[18] NISHIDA H,YOSHIDA T,KAWAKAMI K,et al.Structural variation in the 5′ upstream region of photoperiod-insensitive alleles Ppd-A1a and Ppd-B1a identified in hexaploid wheat (Triticum aestivum L.),and their effect on heading time [J].Molecular Breeding,2013,31(1):27-37.
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[20] ZHANG X K,XIAO Y G,ZHANG Y,et al.Allelic variation at the vernalization genes Vrn-A1,Vrn-B1,Vrn-D1,and Vrn-B3 in Chinese wheat cultivars and their association with growth habit [J].Crop Science,2008,42(2):1690-1694.
[21] 韩领锋,亢玲,张博,等.小麦光周期基因在我国不同麦区中的组成分布 [J].麦类作物学报,2016,36(12):1617-1622.
[22] WORLAND A J.The influence of flowering time genes on environmental adaptability in European wheats [J].Euphytica,1996,89(1):49-57.
[23] 赵虹,胡卫国,詹克慧,等.黄淮南片冬麦区主导品种春化基因及冬春性分析 [J].西北植物学报,2010,30(3):495-504.
[24] 刘政,陈晓杰,胡银岗.春化及光周期基因的等位变异及其对冬小麦光合及产量性状的影响 [J].麦类作物学报,2016,36(6):714-720.
[25] 金善宝.中国小麦学 [M].北京:农业出版社,1996.
[26] 张晓祥,王玲,寿路路.一种快速提取小麦基因组DNA的改良CTAB方法 [J].中国农学通报,2012,28(36):46-49.
[27] 江苏省农科院粮作所品种资源研究所.江苏省小麦大麦品种志 [M].南京:江苏科学技术出版社,1985.
[28] 杨芳萍,韩利明,阎俊,等.春化和光周期基因等位变异在23个国家小麦品种中的分布 [J].作物学报,2011,37(11):1917-1925.
[29] YANG F P,ZHANG X K,XIA X C,et al.Distribution of the photoperiod insensitive Ppd-D1a allele in Chinese wheat cultivars [J].Euphytica,2009,165(3):445-452.
[30] 郭总总,王翔,卫丽,等.黄淮麦区小麦春化基因组成的多态性分布研究 [J].河南农业大学学报,2014,48(3):255-262.
[31] 许世蛟,熊小丽,谢梦洁,等.江苏省小麦品种资源农艺性状演变规律研究 [J].湖南农业科学,2009(9):1-3,7.
[32] 高德荣,张晓,康建鹏,等.长江中下游麦区小麦迟播的不利影响及育种对策 [J].麦类作物学报,2014,34(2):279-283.
[33] GALIBA G,QUARRIE S A,SUTKA J,et al.RFLP mapping of the vernalization (Vrn1) and frost resistance (Fr1) genes on chromosome 5A of wheat [J].Theoretical Applied Genetics,1995,90(7/8):1174.
[2] FLOOD R G,HALLORAN G M.Genetics and physiology of vernalization response in wheat [J].Adv Agr,1986,39(39):87-125.
[3] PUGSLEY A T.A genetic analysis of the spring-winter habit of growth in wheat [J].Australian Journal of Agricultural Research,1971,22(1):21-31.
[4] YAN L,LOUKOIANOV A,BLECHL A,et al.The wheat VRN2 gene is a flowering repressor down-regulated byvernalization [J].Science,2004,303(5664):1640-1644.
[5] DISTELFELD A,TRANQUILLI G,Li C,et al.Genetic and molecular characterization of the VRN2 loci in tetraploid wheat [J].Plant Physiology,2009,149(1):245-257.
[6] YAN L,FU D,LI C,et al.The wheat and barley vernalization gene VRN3 is an orthologue of FT [J].Proceedings of the National Academy of Sciences of the United States of America,2006,103(51):19581-19586.
[7] YOSHIDA T,NISHIDA H,ZHU J,et al.Vrn-D4 is avernalization gene located on the centromeric region of chromosome 5D in hexaploid wheat [J].Theoretical & Applied Genetics,2010,120(3):543-552.
[8] YAN L,HELGUERA M,KATO K,et al.Allelic variation at the VRN-1,promoter region in polyploid wheat [J].Theor Appl Genet,2004,109(8):1677-1686.
[9] YAN L,LOUKOIANOY A,TRANQUILLI G,et al.Positional cloning of the wheat vernalization gene VRN1 [J].Proceedings of the National Academy of Sciences of the United States of America,2003,100(10):6263-6268.
[10] FU D,SZUCS P,YAN L,et al.Large deletions within the first intron in VRN-1 are associated with spring growth habit in barley and wheat [J].Molecular Genetics & Genomics,2005,274(4):442-443.
[11] 姜莹,黄林周,胡银岗.中国小麦地方品种春化基因的分布及其与冬春性的关系 [J].中国农业科学,2010,43(13):2619-2632.
[12] ZHANG J,WANG Y,WU S,et al.A single nucleotide polymorphism at the Vrn-D1 promoter region in common wheat is associated with vernalization response [J].Theoretical Applied Genetics,2012,125(8):1697-1704.
[13] STELMAKH A F.Growth habit in common wheat (Triticum aestivum L.em.Thell.)[J].Euphytica,1987,36(2):513-519.
[14] 王敏慧.小麦春化基因Vrn-1新等位变异发掘 [D].北京:中国农业科学院,2011.
[15] 潘瑞炽.植物生理学 [M].北京:高等教育出版社,2008:276.
[16] WELSH J R,KEIN D L,PIRASTEH B,et al.Genetic control of photoperiod response in wheat [C]// Missouri:Proceeding of the 4th International wheat Genetic Symposium.1973:89
[17] WORLAND A J,BORNER A,KORZUN V,et al.The influence of photoperiod genes on the adaptability of European winter wheats [J].Euphytica,1998,100(1/2/3):385-394.
[18] NISHIDA H,YOSHIDA T,KAWAKAMI K,et al.Structural variation in the 5′ upstream region of photoperiod-insensitive alleles Ppd-A1a and Ppd-B1a identified in hexaploid wheat (Triticum aestivum L.),and their effect on heading time [J].Molecular Breeding,2013,31(1):27-37.
[19] BEALES J,TURNER A,GRIFFITHS S,et al.A pseudo-response regulator,is misexpressed in the photoperiod insensitive Ppd-D1a,mutant of wheat (Triticum aestivum L.) [J].Theoretical Applied Genetics,2007,115(5):721-733.
[20] ZHANG X K,XIAO Y G,ZHANG Y,et al.Allelic variation at the vernalization genes Vrn-A1,Vrn-B1,Vrn-D1,and Vrn-B3 in Chinese wheat cultivars and their association with growth habit [J].Crop Science,2008,42(2):1690-1694.
[21] 韩领锋,亢玲,张博,等.小麦光周期基因在我国不同麦区中的组成分布 [J].麦类作物学报,2016,36(12):1617-1622.
[22] WORLAND A J.The influence of flowering time genes on environmental adaptability in European wheats [J].Euphytica,1996,89(1):49-57.
[23] 赵虹,胡卫国,詹克慧,等.黄淮南片冬麦区主导品种春化基因及冬春性分析 [J].西北植物学报,2010,30(3):495-504.
[24] 刘政,陈晓杰,胡银岗.春化及光周期基因的等位变异及其对冬小麦光合及产量性状的影响 [J].麦类作物学报,2016,36(6):714-720.
[25] 金善宝.中国小麦学 [M].北京:农业出版社,1996.
[26] 张晓祥,王玲,寿路路.一种快速提取小麦基因组DNA的改良CTAB方法 [J].中国农学通报,2012,28(36):46-49.
[27] 江苏省农科院粮作所品种资源研究所.江苏省小麦大麦品种志 [M].南京:江苏科学技术出版社,1985.
[28] 杨芳萍,韩利明,阎俊,等.春化和光周期基因等位变异在23个国家小麦品种中的分布 [J].作物学报,2011,37(11):1917-1925.
[29] YANG F P,ZHANG X K,XIA X C,et al.Distribution of the photoperiod insensitive Ppd-D1a allele in Chinese wheat cultivars [J].Euphytica,2009,165(3):445-452.
[30] 郭总总,王翔,卫丽,等.黄淮麦区小麦春化基因组成的多态性分布研究 [J].河南农业大学学报,2014,48(3):255-262.
[31] 许世蛟,熊小丽,谢梦洁,等.江苏省小麦品种资源农艺性状演变规律研究 [J].湖南农业科学,2009(9):1-3,7.
[32] 高德荣,张晓,康建鹏,等.长江中下游麦区小麦迟播的不利影响及育种对策 [J].麦类作物学报,2014,34(2):279-283.
[33] GALIBA G,QUARRIE S A,SUTKA J,et al.RFLP mapping of the vernalization (Vrn1) and frost resistance (Fr1) genes on chromosome 5A of wheat [J].Theoretical Applied Genetics,1995,90(7/8):1174.