老年大鼠股动脉结扎加股动静脉吻合与单纯股动脉结扎的侧支血管生长情况的差异性分析
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摘要
目的观察老年大鼠单纯股动脉结扎和股动脉结扎加股动静脉吻合对侧支血管生长情况的影响。方法选取36只健康的老年SD大鼠,根据随机数字表法将其分为假手术组(12只)、单纯结扎组(12只)和结扎吻合组(12只)。假手术组大鼠不行股动脉结扎和股动静脉吻合,单纯结扎组大鼠行单纯股动脉结扎,结扎吻合组大鼠行股动脉结扎加股动静脉吻合。所有大鼠手术后存活7d,每组取6只大鼠通过明胶四氧化三铅行血管造影后进行X线拍照对其后肢侧支血管生长情况进行观察,剩下6只取后肢带侧支血管肌肉做冰冻切片,采用免疫荧光组织化学法对Ki67、eNOS及Ang-1进行测定。比较三组大鼠后肢侧支血管数量、Ki67、eNOS及Ang-1免疫荧光强度。结果三组大鼠后肢侧支血管数量、Ki67、eNOS及Ang-1免疫荧光强度存在显著差异(P<0.05);单纯结扎组和结扎吻合组大鼠后肢侧支血管数量、Ki67、eNOS及Ang-1免疫荧光强度显著高于假手术组,差异有统计学意义(P<0.05);结扎吻合组大鼠后肢侧支血管数量、Ki67、eNOS及Ang-1免疫荧光强度显著高于单纯结扎组,差异有统计学意义(P<0.05)。结论血流切应力的提高能够有效促进老年大鼠后肢侧支血管生长,在血管生长过程中,Ki67、eNOS及Ang-1在血管表达增加。
Objective To observe the effect of simple femoral artery ligation and femoral artery ligation plus femoral arteriovenous anastomosis on collateral vessel growth in aged rats.Methods Thirty-six healthy aged SD rats were divided into Sham operation group(n=12),simple ligation group(n=12)and ligation anastomosis group(n=12)according to random digital table method.In sham-operation group,femoral artery ligation and femoral arteriovenous anastomosis were not performed.Simple femoral artery ligation was performed in the simple ligation group,and femoral artery ligation plus femoral arteriovenous anastomosis was performed in the ligation and anastomosis group.All the rats survived 7 days after operation.Six rats in each group were taken by gelatin trilead tetroxide to take X-ray photographs to observe the vascular growth of the lateral branches of their hind limbs.The Ki67,eNOS and Ang-1 were detected by immunofluorescence histochemistry.The number of collateral vessels and the immunofluorescence intensity of Ki67,eNOS and Ang-1 were compared among three groups.Results There were significant differences in the number of collateral vessels,the immunofluorescence intensity of Ki67,eNOS and Ang-1 among the three groups(P<0.05).The number of collateral vessels,the immunofluorescence intensity of Ki67,eNOS and Ang-1 in ligation anastomosis group and simple ligation group were significantly higher than that in Sham operation group,the difference was statistically significant(P<0.05).The number of collateral vessels,the immunofluorescence intensity of Ki67,eNOS and Ang-1 in the ligation and anastomosis group were significantly higher than those in the simple ligation group,the difference was statistically significant(P<0.05).Conclusion The increase of blood flow shear stress can effectively promote the growth of collateral vessels in the hindlimb of aged rats.During the process of vascular growth,the expression of Ki67,eNOS and Ang-1 increases in the blood vessels.
Objective To observe the effect of simple femoral artery ligation and femoral artery ligation plus femoral arteriovenous anastomosis on collateral vessel growth in aged rats.Methods Thirty-six healthy aged SD rats were divided into Sham operation group(n=12),simple ligation group(n=12)and ligation anastomosis group(n=12)according to random digital table method.In sham-operation group,femoral artery ligation and femoral arteriovenous anastomosis were not performed.Simple femoral artery ligation was performed in the simple ligation group,and femoral artery ligation plus femoral arteriovenous anastomosis was performed in the ligation and anastomosis group.All the rats survived 7 days after operation.Six rats in each group were taken by gelatin trilead tetroxide to take X-ray photographs to observe the vascular growth of the lateral branches of their hind limbs.The Ki67,eNOS and Ang-1 were detected by immunofluorescence histochemistry.The number of collateral vessels and the immunofluorescence intensity of Ki67,eNOS and Ang-1 were compared among three groups.Results There were significant differences in the number of collateral vessels,the immunofluorescence intensity of Ki67,eNOS and Ang-1 among the three groups(P<0.05).The number of collateral vessels,the immunofluorescence intensity of Ki67,eNOS and Ang-1 in ligation anastomosis group and simple ligation group were significantly higher than that in Sham operation group,the difference was statistically significant(P<0.05).The number of collateral vessels,the immunofluorescence intensity of Ki67,eNOS and Ang-1 in the ligation and anastomosis group were significantly higher than those in the simple ligation group,the difference was statistically significant(P<0.05).Conclusion The increase of blood flow shear stress can effectively promote the growth of collateral vessels in the hindlimb of aged rats.During the process of vascular growth,the expression of Ki67,eNOS and Ang-1 increases in the blood vessels.
引文
[1] 蒋超,王月娟,张静,等.急性颈内动脉系统脑梗死患者侧支循环建立情况与预后关系的研究[J].中华行为医学与脑科学杂志,2017,26(7):615-619.
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[10] 蔡百桢,叶峰,罗明英,等.兔后肢高血流切应力诱导的侧支血管 CCR2 的表达及其与单核/巨噬细胞的关系[J].中国动脉硬化杂志,2015(11):1089-1092.
[11] 李玲,陆晓,励建安,等.血管内皮生长因子和一氧化氮对兔生理性缺血冠状动脉侧支血流量的影响[J].中华物理医学与康复杂志,2013,35(4):241-244.
[12] 李鹏,冯毅.一氧化氮在心血管疾病中的作用[J].医学综述,2010,16(1):31-33.
[13] 彭丹,左后娟,秦瑾,等.CD151蛋白促内皮细胞增殖和eNOS蛋白表达[J].中国组织化学与细胞化学杂志,2011,20(1):1-5.
[14] 石菲,王永春,杜挺媛,等.回转模拟失重对静脉内皮细胞血管生成能力的影响及内皮型一氧化氮合成酶的作用[J].航天医学与医学工程,2013,26(2):83-86.
[15] 唐春花,郭淮莲.血管生长素与治疗性血管生成的研究进展[J].生理科学进展,2011,42(3):210-213.
[16] 刘帅,刘学政.Ang-1/Tie2系统与病理性血管形成的关系[J].解剖科学进展,2010,16(1):85-88.
[17] 郭宜竞,邱要颜,蔡维君.老年大鼠后肢高血流剪切力诱导的侧支血管生长及其与血管生成素-1表达的关系[J].解剖学杂志,2017,40(2):145-148.
[18] 周召锋,徐晤,张超群,等.白藜芦醇对AMI后心脏血管新生及冠脉侧支循环影响的实验研究[J].山东医药,2010,50(23):42-43.
[2] 代德辉,陈青云.护理干预对老年心力衰竭患者治疗依从性及预后的影响[J].中国实用护理杂志,2011,27(36):11-12.
[3] 王改琴,景鹏,张旭东,等.冠状动脉侧支血管生长发育[J].解剖科学进展,2016,22(2):199-201.
[4] 陆四.冠状动脉侧支循环的研究进展[J].医学综述,2014,20(12):2113-2115.
[5] 谢应桂,童建斌,伍校琼,等.血流切应力的增加而非缺血可能是启动侧支血管生长发育的主要因素[J].中国现代医学杂志,2007,17(17):2099-2101.
[6] 黄庆红,王岐本,谢应桂,等.兔股动脉结扎诱导动脉生成过程中iNOS和eNOS的表达[J].中国临床解剖学杂志,2009,27(4):433-435.
[7] 罗明英,邹智荣,范艳,等.去神经支配对鼠后肢缺血诱导的侧支血管管壁激活的影响[J].中国组织化学与细胞化学杂志,2014,23(5):428-432.
[8] 姜波,辛世杰.血管重塑机制研究进展[J].解剖科学进展,2015,21(4):408-411.
[9] 张洪,杨旭明,周华顶.影响冠状动脉侧支循环形成的因素分析[J].心血管康复医学杂志,2016,25(4):364-367.
[10] 蔡百桢,叶峰,罗明英,等.兔后肢高血流切应力诱导的侧支血管 CCR2 的表达及其与单核/巨噬细胞的关系[J].中国动脉硬化杂志,2015(11):1089-1092.
[11] 李玲,陆晓,励建安,等.血管内皮生长因子和一氧化氮对兔生理性缺血冠状动脉侧支血流量的影响[J].中华物理医学与康复杂志,2013,35(4):241-244.
[12] 李鹏,冯毅.一氧化氮在心血管疾病中的作用[J].医学综述,2010,16(1):31-33.
[13] 彭丹,左后娟,秦瑾,等.CD151蛋白促内皮细胞增殖和eNOS蛋白表达[J].中国组织化学与细胞化学杂志,2011,20(1):1-5.
[14] 石菲,王永春,杜挺媛,等.回转模拟失重对静脉内皮细胞血管生成能力的影响及内皮型一氧化氮合成酶的作用[J].航天医学与医学工程,2013,26(2):83-86.
[15] 唐春花,郭淮莲.血管生长素与治疗性血管生成的研究进展[J].生理科学进展,2011,42(3):210-213.
[16] 刘帅,刘学政.Ang-1/Tie2系统与病理性血管形成的关系[J].解剖科学进展,2010,16(1):85-88.
[17] 郭宜竞,邱要颜,蔡维君.老年大鼠后肢高血流剪切力诱导的侧支血管生长及其与血管生成素-1表达的关系[J].解剖学杂志,2017,40(2):145-148.
[18] 周召锋,徐晤,张超群,等.白藜芦醇对AMI后心脏血管新生及冠脉侧支循环影响的实验研究[J].山东医药,2010,50(23):42-43.