猪布鲁菌感染对巨噬细胞泛素蛋白酶体功能的影响
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摘要
巨噬细胞(macrophage,M_φ)是布鲁菌的主要宿主细胞,为了探明布鲁菌感染与M_φ泛素-蛋白酶体功能之间的关系,本试验在应用蛋白酶体抑制剂Lactacystin与促进剂IFN-γ的基础上,对M_φ感染猪种布鲁菌S2株(Brucella suis,B.suis)后细胞上清中泛素、蛋白酶体及胞内B.suis数量进行了检测。结果显示,IFN-γ与Lactacystin对M_φ泛素的表达均有明显的促进作用,且IFN-γ效果显著优于Lactacystin;其次IFN-γ有效促进了M_φ蛋白酶体的表达,而Lactacystin显著抑制了M_φ蛋白酶体的表达。在此基础上,将B.suis感染不同状态的M_φ,在0.5~24h分别进行胞内B.suis计数,研究表明,一方面B.suis的感染促进了M_φ泛素及蛋白酶体的表达;另一方面,泛素蛋白酶体系统功能的增强,显著降低了B.suis的早期感染,但对于B.suis感染中后期作用不明显,而感染后期抑制M_φ蛋白酶体功能,却可显著降低B.suis的胞内增殖能力。
M_φ is the main host cell of Brucella.In order to study the relationship between Brucella and ubiquitin proteasome system in M_φ,the ubiquitin,proteasome and the number of intracellular B.suis were tested respectively after application of proteasome inhibitor lactacystin and promoter IFN-gamma to treat M_φ .The results showed that IFN-γand lactacystin both increased the ubiquitin markedly,and the function of IFN-γwas more significant.The IFN-γpromoted the expression of proteasome in M_φ significantly,but the lactacystin inhibited its expression.The number of intracellular B.suis was counted after B.suis affected M_φ treated by IFN-γand Lactacystin from 0.5 h to 24 hrespectively.The results showed that B.suis infection promoted the expression of ubiquitin and proteasome in M_φ .On the other hand,strengthening function of ubiquitin proteasome system reduced B.suis early infection in M_φ significantly,but the function was not obvious for B.suis infection in the later stage,indicating that the inhibition of proteasome function might restraine the proliferation ability of B.suis in M_φ .
M_φ is the main host cell of Brucella.In order to study the relationship between Brucella and ubiquitin proteasome system in M_φ,the ubiquitin,proteasome and the number of intracellular B.suis were tested respectively after application of proteasome inhibitor lactacystin and promoter IFN-gamma to treat M_φ .The results showed that IFN-γand lactacystin both increased the ubiquitin markedly,and the function of IFN-γwas more significant.The IFN-γpromoted the expression of proteasome in M_φ significantly,but the lactacystin inhibited its expression.The number of intracellular B.suis was counted after B.suis affected M_φ treated by IFN-γand Lactacystin from 0.5 h to 24 hrespectively.The results showed that B.suis infection promoted the expression of ubiquitin and proteasome in M_φ .On the other hand,strengthening function of ubiquitin proteasome system reduced B.suis early infection in M_φ significantly,but the function was not obvious for B.suis infection in the later stage,indicating that the inhibition of proteasome function might restraine the proliferation ability of B.suis in M_φ .
引文
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[21]DERETIC V.Autophagy in immunity and cell-autonomous defense against intracellular microbes[J].Immunol Rev,2011,240(1):92-104.
[2]CELLI J.Surviving inside a macrophage:the many ways of Brucella[J].Res Microbiol,2006,157(2):93-98.
[3]SA J C,SILVA T M,COSTA E A,et al.The virB-encoded typeⅣsecretion system is critical for establishment of infection and persistence of Brucella ovis infection in mice[J].Vet Microbiol,2012,159(1/2):130-140.
[4]DAVID R.Cellular microbiology:an unconventional exit for Brucella[J].Nat Rev Microbiol,2012,10(3):160.
[5]BARRIONUEVO P,DELPINO M V,POZNER R G,et al.Brucella abortus induces intracellular retention of MHC-I molecules in human macrophages downmodulating cytotoxic CD8(+)T cell responses[J].Cell Microbiol,2013,15(4):487-502.
[6]LAPAQUE N,MULLER A,ALEXOPOULOU L,et al.Brucella abortus induces Irgm3and Irga6expression via type-I IFN by a MyD88-dependent pathway,without the requirement of TLR2,TLR4,TLR5and TLR9[J].Microb Pathog,2009,47(6):299-304.
[7]SKENDROS P,PAPPAS G,BOURA P,et al.Cellmediated immunity in human Brucellosis[J].Microbes Infect,2011,13(2):134-142.
[8]权伍荣,杨咏洁.布鲁氏菌逃逸宿主的抗感染免疫机制[J].微生物学报,2016,56(5):747-752.
[9]GRICE G L,NATHAN J A.The recognition of ubiquitinated proteins by the proteasome[J].Cell Mol Life Sci,2016,73(18):3497-3506.
[10]DIAZ-HERNANDEZ M,MARTIN-APARICIO E,AVILA J,et al.Enhanced induction of the immunoproteasome by interferon gamma in neurons expressing mutant Huntingtin[J].Neurotox Res,2004,6(6):463-468.
[11]RIVETT A J,HEARN A R.Proteasome function in antigen presentation:immunoproteasome complexes,Peptide production,and interactions with viral proteins[J].Curr Protein Pept Sci,2004,5(3):153-161.
[12]HAORAH J,HEILMAN D,DIEKMANN C,et al.Alcohol and HIV decrease proteasome and immunoproteasome function in macrophages:implications for impaired immune function during disease[J].Cell Immunol,2004,229(2):139-148.
[13]OSNA N A,CLEMENS D L,DONOHUE T M Jr,et al.Interferon gamma enhances proteasome activity in recombinant Hep G2cells that express cytochrome P4502E1:modulation by ethanol[J].Biochem Pharmacol,2003,66(5):697-710.
[14]LIVNEH I,COHEN-KAPLAN V,COHEN-ROSENZWEIG C,et al.The life cycle of the 26Sproteasome:from birth,through regulation and function,and onto its death[J].Cell Res,2016,26(8):869-885.
[15]UNNO M,MIZUSHIMA T,MORIMOTO Y,et al.The structure of the mammalian 20Sproteasome at 2.75Aresolution[J].Structure,2002,10(5):609-618.
[16]COHEN-KAPLAN V,LIVNEH I,AVNI N,et al.The ubiquitin-proteasome system and autophagy:Coordinated and independent activities[J].Int J Biochem Cell Biol,2016,2725(16):30193-30195.
[17]李明,缪泽鸿,丁健.蛋白酶体及其抑制剂的研究进展[J].中国药理学通报,2008,24(5):565-569.
[18]LUO H.Interplay between the virus and the ubiquitinproteasome system:molecular mechanism of viral pathogenesis[J].Curr Opin Virol,2016,17:1-10.
[19]HE K,RAVINDRAN M S,TSAI B,et al.A bacterial toxin and a nonenveloped virus hijack ER-to-cytosol membrane translocation pathways to cause disease[J].Crit Rev Biochem Mol Biol,2015,50(6):477-488.
[20]MINOR M M,SLAGLE B L.Hepatitis B virus HBx protein interactions with the ubiquitin proteasome system[J].Viruses,2014,6(11):4683-4702.
[21]DERETIC V.Autophagy in immunity and cell-autonomous defense against intracellular microbes[J].Immunol Rev,2011,240(1):92-104.