灰霉胞外分泌物对蓝莓果实贮藏品质的影响
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摘要
灰霉在侵染蓝莓果实的过程中会分泌胞外分泌物,并在贮藏过程中对蓝莓品质造成影响。本文研究灰霉胞外分泌物对果实采后贮藏品质的影响。以"莱克西"蓝莓品种为试材,蓝莓果实经过灰霉胞外分泌物处理后,在25℃条件下贮藏。结果表明:在贮藏过程中,灰霉胞外分泌物使蓝莓果实的可溶性固形物(TSS)和可滴定酸(TA)维生素C、总酚和花色苷含量等营养指标下降,且含量始终低于同时期的对照组。而超氧阴离子则随着贮藏时间的延长呈上升趋势。蓝莓果实的PAL、SOD、POD和CAT等抗氧化酶活性先上升后下降,且灰霉胞外分泌物组的蓝莓果实酶活高峰比对照组早2 d达到,而后期酶活始终低于对照组,说明灰霉胞外分泌物导致蓝莓贮藏过程中品质的下降,其商品价值降低。
In the process of infecting blueberry fruit, ash moldy secretes extracellular secretions and affects the quality of blueberries during storage. This paper mainly studies the influence on storage quality of Postharvest Botrytis extracellular secretion, with "Lexie" blueberry varieties of blueberry fruit after B.cinerea extracellular secretion after storage under the condition of 25 DEG C. The results show that: in the process of storage, Botrytis extracellular secretion will make soluble blueberry fruit solids(TSS) and titratable acid(TA) prolonged vitamin C, total phenolic and anthocyanin content of the nutrition index with the storage time decreased, and the content is always lower than the control group at the same time period. The superoxide anion increased with the prolongation of storage time. The antioxidant activity of PAL, SOD, POD and CAT in blueberry fruits first increased and then decreased. And the peak of enzyme activity in blueberry fruit exudates was 2 days earlier than that in control group, but the activity of enzyme activity in blueberry fruits was always lower than that in control group. It is indicated that the exudates from the exudate of grey moldy will lead to the decrease of the quality of the blueberry and the value of its commodity during the storage of blueberry.
In the process of infecting blueberry fruit, ash moldy secretes extracellular secretions and affects the quality of blueberries during storage. This paper mainly studies the influence on storage quality of Postharvest Botrytis extracellular secretion, with "Lexie" blueberry varieties of blueberry fruit after B.cinerea extracellular secretion after storage under the condition of 25 DEG C. The results show that: in the process of storage, Botrytis extracellular secretion will make soluble blueberry fruit solids(TSS) and titratable acid(TA) prolonged vitamin C, total phenolic and anthocyanin content of the nutrition index with the storage time decreased, and the content is always lower than the control group at the same time period. The superoxide anion increased with the prolongation of storage time. The antioxidant activity of PAL, SOD, POD and CAT in blueberry fruits first increased and then decreased. And the peak of enzyme activity in blueberry fruit exudates was 2 days earlier than that in control group, but the activity of enzyme activity in blueberry fruits was always lower than that in control group. It is indicated that the exudates from the exudate of grey moldy will lead to the decrease of the quality of the blueberry and the value of its commodity during the storage of blueberry.
引文
[1]PATEL S.Blueberry as functional food and dietary supplement:The natural way to ensure holistic health[J].Mediterranean Journal of Nutrition&Metabolism,2014,7(1):133-143.
[2]卜庆雁,周晏起.浅析蓝莓的营养保健功能及开发利用前景[J].北方园艺,2010,25(8):215-217.
[3]YANG G,YUE J,GONG X,et al.Blueberry leaf extracts incorporated chitosan coatings for preserving postharvest quality of fresh blueberries[J].Postharvest Biology&Technology,2014,92(2):46-53.
[4]MEHRA L K,SAVELLE A T,MACLEAN D D,et al.Postharvest disease development on southern highbush blueberry fruit in relation to berry flesh type and harvest method[J].Plant Disease,2013,97(2):213-221.
[5]REHM B H.Bacterial polymers:biosynthesis,modifications and applications[J].Nature Reviews Microbiology,2010,8(8):578.
[6]郜海燕,肖尚月,陈杭君,等.蓝莓采后主要病原真菌的分离鉴定与生物学特性研究[J].农业机械学报,2017,48(5):327-334.
[7]赵红霞,苟萍.灰葡萄孢菌致病机理研究进展[J].生物技术,2014,24(1):100-103.
[8]VOIGT C A,SCH魧FER W,SALOMON S.A secreted lipase of Fusarium graminearum is a virulence factor required for infection of cereals[J].Plant Journal for Cell&Molecular Biology,2005,42(3):364-375.
[9]杨帅,郜海燕,陈杭君,等.蓝莓外表皮蜡质损伤对果实品质的影响[C]//中国食品科学技术学会年会论文摘要集.无锡:中国食品科学技术学会,2014.
[10]LARA I,BELGE B,GOULAO L F.The fruit cuticle as a modulator of postharvest quality[J].Postharvest Biology&Technology,2014,87(1):103-112.
[11]EDELMANN H G,NEINHUIS C,BARGEL H.Influence of hydration and temperature on the rheological properties of plant cuticles and their impact on plant organ integrity[J].Journal of Plant Growth Regulation,2005,24(2):116-126.
[12]孙媛.灰霉角质酶的致病作用及角质酶突变菌株的基因分析[D].扬州:扬州大学,2011.
[13]CHENG H,CHEN J L,LI X,et al.Differentiation of the volatile profiles of Chinese bayberry cultivars during storage by HS-SPME-GC/MS combined with principal component analysis[J].Postharvest Biology&Technology,2015,100(1):59-72.
[14]杜朝昆,李忠光,龚明.水杨酸诱导的玉米幼苗适应高温和低温胁迫的能力与抗氧化酶系统的关系[J].植物生理学报,2005,41(1):19-22.
[15]胡位荣,张昭其,季作梁,等.荔枝冷害过程中果皮色泽、花色素苷和类黄酮含量的变化[J].园艺学报,2004,31(6):723-726.
[16]李巨秀,王柏玉.福林-酚比色法测定桑椹中总多酚[J].食品科学,2009,30(18):292-295.
[17]曹建康,姜微波,赵玉梅.果蔬采后生理生化实验指导[M].北京:中国轻工业出版社,2007:142-144.
[18]胡琼英,狄洌.生物化学实验[M].北京:北京大学出版社,2007:80-83.
[19]唐燕,杜光源,张继澍.猕猴桃贮藏期电参数和生理参数的变化[J].农业机械学报,2012,43(1):127-133.
[20]龚佳惠,陈贝莉,董欢欢,等.L-精氨酸对绿芦笋贮藏品质及生理生化特性的影响[J].保鲜与加工,2017(1):7-11.
[21]周晓慧,WOLUKAU N,李英,等.甜瓜蔓枯病抗性与SOD、CAT和POD活性变化的关系[J].中国瓜菜,2007,2(2):4-6.
[22]钟玮迪,李学文,张瑞杰,等.果皮脱蜡对库尔勒香梨采后生理活性的影响[J].新疆农业科学,2016,53(7):1223-1229.
[23]李珍慈.库尔勒香梨表皮蜡质成分分析及其在贮藏中的影响研究[D].石河子:石河子大学,2016.
[24]刘娜,蔡丛希,孙勃,等.核盘菌侵染拟南芥的过程及其芥子油苷的变化[J].核农学报,2011,25(2):382-390.
[25]宋会歌,刘美艳,董楠,等.食品中花色苷降解机制研究进展[J].食品科学,2011,32(13):355-359.
[26]LI Q.Effect of carbon monoxide fumigation on the respiration and oxidase activities of postharvest Jujube[J].Journal of Plant Sciences,2014,2(4):139.
[2]卜庆雁,周晏起.浅析蓝莓的营养保健功能及开发利用前景[J].北方园艺,2010,25(8):215-217.
[3]YANG G,YUE J,GONG X,et al.Blueberry leaf extracts incorporated chitosan coatings for preserving postharvest quality of fresh blueberries[J].Postharvest Biology&Technology,2014,92(2):46-53.
[4]MEHRA L K,SAVELLE A T,MACLEAN D D,et al.Postharvest disease development on southern highbush blueberry fruit in relation to berry flesh type and harvest method[J].Plant Disease,2013,97(2):213-221.
[5]REHM B H.Bacterial polymers:biosynthesis,modifications and applications[J].Nature Reviews Microbiology,2010,8(8):578.
[6]郜海燕,肖尚月,陈杭君,等.蓝莓采后主要病原真菌的分离鉴定与生物学特性研究[J].农业机械学报,2017,48(5):327-334.
[7]赵红霞,苟萍.灰葡萄孢菌致病机理研究进展[J].生物技术,2014,24(1):100-103.
[8]VOIGT C A,SCH魧FER W,SALOMON S.A secreted lipase of Fusarium graminearum is a virulence factor required for infection of cereals[J].Plant Journal for Cell&Molecular Biology,2005,42(3):364-375.
[9]杨帅,郜海燕,陈杭君,等.蓝莓外表皮蜡质损伤对果实品质的影响[C]//中国食品科学技术学会年会论文摘要集.无锡:中国食品科学技术学会,2014.
[10]LARA I,BELGE B,GOULAO L F.The fruit cuticle as a modulator of postharvest quality[J].Postharvest Biology&Technology,2014,87(1):103-112.
[11]EDELMANN H G,NEINHUIS C,BARGEL H.Influence of hydration and temperature on the rheological properties of plant cuticles and their impact on plant organ integrity[J].Journal of Plant Growth Regulation,2005,24(2):116-126.
[12]孙媛.灰霉角质酶的致病作用及角质酶突变菌株的基因分析[D].扬州:扬州大学,2011.
[13]CHENG H,CHEN J L,LI X,et al.Differentiation of the volatile profiles of Chinese bayberry cultivars during storage by HS-SPME-GC/MS combined with principal component analysis[J].Postharvest Biology&Technology,2015,100(1):59-72.
[14]杜朝昆,李忠光,龚明.水杨酸诱导的玉米幼苗适应高温和低温胁迫的能力与抗氧化酶系统的关系[J].植物生理学报,2005,41(1):19-22.
[15]胡位荣,张昭其,季作梁,等.荔枝冷害过程中果皮色泽、花色素苷和类黄酮含量的变化[J].园艺学报,2004,31(6):723-726.
[16]李巨秀,王柏玉.福林-酚比色法测定桑椹中总多酚[J].食品科学,2009,30(18):292-295.
[17]曹建康,姜微波,赵玉梅.果蔬采后生理生化实验指导[M].北京:中国轻工业出版社,2007:142-144.
[18]胡琼英,狄洌.生物化学实验[M].北京:北京大学出版社,2007:80-83.
[19]唐燕,杜光源,张继澍.猕猴桃贮藏期电参数和生理参数的变化[J].农业机械学报,2012,43(1):127-133.
[20]龚佳惠,陈贝莉,董欢欢,等.L-精氨酸对绿芦笋贮藏品质及生理生化特性的影响[J].保鲜与加工,2017(1):7-11.
[21]周晓慧,WOLUKAU N,李英,等.甜瓜蔓枯病抗性与SOD、CAT和POD活性变化的关系[J].中国瓜菜,2007,2(2):4-6.
[22]钟玮迪,李学文,张瑞杰,等.果皮脱蜡对库尔勒香梨采后生理活性的影响[J].新疆农业科学,2016,53(7):1223-1229.
[23]李珍慈.库尔勒香梨表皮蜡质成分分析及其在贮藏中的影响研究[D].石河子:石河子大学,2016.
[24]刘娜,蔡丛希,孙勃,等.核盘菌侵染拟南芥的过程及其芥子油苷的变化[J].核农学报,2011,25(2):382-390.
[25]宋会歌,刘美艳,董楠,等.食品中花色苷降解机制研究进展[J].食品科学,2011,32(13):355-359.
[26]LI Q.Effect of carbon monoxide fumigation on the respiration and oxidase activities of postharvest Jujube[J].Journal of Plant Sciences,2014,2(4):139.