细胞外ATP和水杨酸对烟草光合指标的影响
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摘要
研究了细胞外ATP(eATP)和水杨酸(SA)对烟草(Nicotiana tabacum)叶片的气孔导度(GH2O)、蒸腾速率(E)、光合作用速率(A)与叶绿素荧光参数[包括PSⅡ潜在最大光化学量子效率(Fv/Fm)、PSⅡ光适应下实际光化学效率Y(Ⅱ)、电子传递速率(ETR)、非光化学荧光淬灭(NPQ)和光化学荧光淬灭(qP)]的影响。结果表明:SA能导致A、GH2O和E的下降,而eATP的处理能缓解SA造成的A、GH2O和E的下降;但SA未对叶绿素荧光参数Fv/Fm、Y(Ⅱ)、NPQ、qP和ETR造成显著影响,eATP的加入也未改变SA处理下叶片叶绿素荧光参数的水平。这说明SA能导致光合作用的抑制,而eATP能明显缓解SA对光合作用的抑制,但以上作用可能均和光反应阶段无关。并对其内在机理进行了探讨。
The present work studied the effects of extracellular ATP(eATP)and salicylic acid(SA)on transpiration rate(E),stomatal conductance(GH2O),photosynthesis rate(A)and chlorophyll fluorescence parameters,including maximal photochemical efficiency of PSⅡ(Fv/Fm),effective photochemical quantum yield of PSⅡ(Y(Ⅱ)),PSⅡ electron transport rate(ETR),non-photochemical quenching coefficient(NPQ)and the coefficient of photochemical fluorescence(qP))of tobacco(Nicotiana tabacum).The results showed that the transpiration rate(E),stomatal conductance(GH2O)and photosynthesis rate(A)decreased under SA stress,while these decreases were anesised by the addition of eATP.However,SA had no significant effects on the chlorophyll fluorescence parameters,and the addition of eATP did not significantly changed the values of the chlorophyll fluorescence parameters under SA stress.These results indicated that SA could inhibit photosynthesis of tobacco leaves and the addition of eATP could alleviate the inhibition of photosynthesis by SA stress,while these effects were not related to the light reaction.We also discussed the possible mechanism based on these observations.
The present work studied the effects of extracellular ATP(eATP)and salicylic acid(SA)on transpiration rate(E),stomatal conductance(GH2O),photosynthesis rate(A)and chlorophyll fluorescence parameters,including maximal photochemical efficiency of PSⅡ(Fv/Fm),effective photochemical quantum yield of PSⅡ(Y(Ⅱ)),PSⅡ electron transport rate(ETR),non-photochemical quenching coefficient(NPQ)and the coefficient of photochemical fluorescence(qP))of tobacco(Nicotiana tabacum).The results showed that the transpiration rate(E),stomatal conductance(GH2O)and photosynthesis rate(A)decreased under SA stress,while these decreases were anesised by the addition of eATP.However,SA had no significant effects on the chlorophyll fluorescence parameters,and the addition of eATP did not significantly changed the values of the chlorophyll fluorescence parameters under SA stress.These results indicated that SA could inhibit photosynthesis of tobacco leaves and the addition of eATP could alleviate the inhibition of photosynthesis by SA stress,while these effects were not related to the light reaction.We also discussed the possible mechanism based on these observations.
引文
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Shen ZX(沈竹夏).2010.The study on the mechanisms of regulation for stomatal movements by calcium signals(钙信号对气孔调控作用机制的探讨)[D].Hangzhou(杭州):Zhejiang University(浙江大学)
Song CJ,Steinebrunner I,Wang XZ,et al.2006.ExtracellularATP induces the accumulation of superoxide via NADPH oxidases in Arabidopsis[J].Plant Physiol,140(4):1 222-1 232
Sun J,Zhang CL,Deng SR,et al.2012.An ATP signalingpathway in plant cells:extracellular ATP triggers programmed cell death in Populus euphratica[J].Plant CellEnviron,35(5):893-916
Wolf C,Hennig M,Romanovicz D,et al.2007.Developmentaldefects and seedling lethality in apyrase AtAPY1and AtAPY2double knockout mutants[J].Plant Mol Biol,64(6):657-672
Yuan S,Lin HZ.2008.Role of salicylic acid in plant abiotic stress[J].Z Naturforsch C,63(5/6):313-320
Zhou X(周欣),Chen SN(陈善娜).2001.Advance in studying ofsalicylic acidon induce resistance in plant(水杨酸在植物诱导抗性方面研究进展)[J].J Yunnan Univ:Nat Sci Edit(云南大学学学报·自然科学版),23(S1):106-108
Zhong C(钟楚),Zhu Y(朱勇).2013.Response of stomatalconductance to light in tobacco plants(烟草气孔导度对光强的响应)[J].Chin J Eco-Agric(中国生态农业学报),21(8):966-972
Bilgin DD,Zavala JA,Jin Z.2010.Biotic stress globally down regulates photosynthesis genes[J].Plant Cell Environ,33(10):1 597-1 613
Chen ZQ(陈志强).2010.Study on the effects of exogenous salicylic acid on the oxidative burst a nd on photosynthesis and celldeath in Arabidopsis leaves(外源水杨酸诱导的活性氧迸发对拟南芥光合作用和细胞死亡的影响研究)[D].Guangzhou(广州):South China Normal University(华南师范大学)
Chivasa S,Simon WJ,Murphy AM.2010.The effects of extracellular adenosine 5-triphosphate on the tobacco proteome[J].Proteomics,10(2):235-244
Clark G,Torres J,Finlayson S,et al.2010.Apyrase(nucleosidetriphosphate diphosphohydrolase)and extracellular nucleotidesregulate cotton fiber elongation in cultured ovules[J].PlantPhysiol,152(2):1 073-1 083
Demidchik V,Shang Z,Shin R,et al.2011.Receptor-like activity evoked by extracellular ADP in Arabidopsis thalianaroot epidermal plasma membrane[J].Plant Physiol,156(3):1 375-1 385
Genty B,Briantais JM,Baker NR.1989.The relationship betweenthe quantum yield of photosynthetic electron transport andquenching of chlorophyll fluorescence[J].Biochem Biophys Acta,990(1):87-92
Hao BZ(蒿宝珍),Jiang LN(姜丽娜),Zhang H(张菡),et al.2011.Irrigation management effects on photosynthetic charateristics of flag leaf and grain yield of winter wheat in northernhenan province(水分运筹对豫北冬小麦生育后期光合特性及产量的影响)[J].Chin Agric Sci Bull(中国农学通报),27(18):180-184
Jiang YP(姜玉萍).2010.Brassinosteroids regulate CO2assimilation through redox changes in Cucuis sativus(氧化还原信号介导的表油菜素内酯对黄瓜光合作用的调控)[D].Hangzhou(杭州):Zhejiang University(浙江大学)
Jia WS,Zhang JH.2008.Stomatal movements and long-distancesignaling in plants[J].Plant Signal Behav,3(10):772-777
Meng XJ(孟雪娇),Di K(邸昆),Ding GH(丁国华).2010.Progress of study on the physiological role of salicylic acid in plant(水杨酸在植物体内的生理作用研究进展)[J].Chin AgricSci Bull(中国农学通报),26(15):207-214
Shen ZX(沈竹夏).2010.The study on the mechanisms of regulation for stomatal movements by calcium signals(钙信号对气孔调控作用机制的探讨)[D].Hangzhou(杭州):Zhejiang University(浙江大学)
Song CJ,Steinebrunner I,Wang XZ,et al.2006.ExtracellularATP induces the accumulation of superoxide via NADPH oxidases in Arabidopsis[J].Plant Physiol,140(4):1 222-1 232
Sun J,Zhang CL,Deng SR,et al.2012.An ATP signalingpathway in plant cells:extracellular ATP triggers programmed cell death in Populus euphratica[J].Plant CellEnviron,35(5):893-916
Wolf C,Hennig M,Romanovicz D,et al.2007.Developmentaldefects and seedling lethality in apyrase AtAPY1and AtAPY2double knockout mutants[J].Plant Mol Biol,64(6):657-672
Yuan S,Lin HZ.2008.Role of salicylic acid in plant abiotic stress[J].Z Naturforsch C,63(5/6):313-320
Zhou X(周欣),Chen SN(陈善娜).2001.Advance in studying ofsalicylic acidon induce resistance in plant(水杨酸在植物诱导抗性方面研究进展)[J].J Yunnan Univ:Nat Sci Edit(云南大学学学报·自然科学版),23(S1):106-108
Zhong C(钟楚),Zhu Y(朱勇).2013.Response of stomatalconductance to light in tobacco plants(烟草气孔导度对光强的响应)[J].Chin J Eco-Agric(中国生态农业学报),21(8):966-972