细胞外ATP对低温下菜豆叶片光系统Ⅱ运行及光能分配的影响
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摘要
细胞外ATP(eATP)可作为一种重要的胞外分子调节植物的多种生理反应。该研究以菜豆(Phaseolus vulgaris L.)品种‘农普12号’幼苗叶片为实验材料,研究了胞外1.0mmol/L ATP溶液对低温下(10℃、10h)菜豆叶片光系统Ⅱ(PSⅡ)运行及光能分配的影响。结果显示:(1)低温胁迫(10℃、10h)对菜豆叶片PSⅡ的潜在最大光化学效率(F_v/F_m)没有产生显著性影响,但低温胁迫却降低了叶片的实际光化学运行效率[Y(Ⅱ)],同时降低了PSⅡ的光化学反应能量(P),增加了天线热耗散能量(D)和非光化学反应耗散能量(E),使叶片胞外ATP水平有所下降。(2)叶片施加外源ATP可以有效缓解低温胁迫下菜豆叶片胞外ATP水平的下降,同时使叶片的实际光化学效率[Y(Ⅱ)]和光化学反应能量(P)显著上升,也使天线热耗散能量(D)非光化学反应耗散能量(E)均显著下降。研究表明,低温胁迫降低了菜豆叶片PSⅡ的光化学运行并增加了光能的耗散,而在低温胁迫下增加胞外ATP水平能够有效提高菜豆叶片光化学反应的运行效率并降低光能的耗散。
It has been established that extracellular ATP(eATP)is an important extracellular molecule required to regulate many physiological responses of plants.This study investigates the effects of eATP on the operation of photosystem Ⅱand the allocation of absorbed light of bean(Phaseolus vulgaris L)leaves under low temperature.The results showed that although low temperature did not have significant effect on the potential maximal photochemical efficiency of PSⅡ(F_v/F_m),low temperature decreased the values of the effective photochemical quantum yield of PSⅡ [Y(Ⅱ)].Further works showed that low temperature decreased the fraction of photochemical efficiency(P)but increased the fraction of antenna heat dissipation(D)and excess energy(E).The level of eATP decreased under low temperature.When the application of exogenous ATP alleviated the decreases of the eATP level under low temperature,the values of Y(Ⅱ)and P were significantly increased,while the values of Dand E were decreased.These results indicate that low temperature caused a decrease in the operation of the PSⅡ photochemistry and caused an increase in dissipation of light energy,and eATP could have function in enhancing the performance of PSⅡ photochemistryand decreasing the dissipation of light energy under the condition of low temperature.
It has been established that extracellular ATP(eATP)is an important extracellular molecule required to regulate many physiological responses of plants.This study investigates the effects of eATP on the operation of photosystem Ⅱand the allocation of absorbed light of bean(Phaseolus vulgaris L)leaves under low temperature.The results showed that although low temperature did not have significant effect on the potential maximal photochemical efficiency of PSⅡ(F_v/F_m),low temperature decreased the values of the effective photochemical quantum yield of PSⅡ [Y(Ⅱ)].Further works showed that low temperature decreased the fraction of photochemical efficiency(P)but increased the fraction of antenna heat dissipation(D)and excess energy(E).The level of eATP decreased under low temperature.When the application of exogenous ATP alleviated the decreases of the eATP level under low temperature,the values of Y(Ⅱ)and P were significantly increased,while the values of Dand E were decreased.These results indicate that low temperature caused a decrease in the operation of the PSⅡ photochemistry and caused an increase in dissipation of light energy,and eATP could have function in enhancing the performance of PSⅡ photochemistryand decreasing the dissipation of light energy under the condition of low temperature.
引文
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[2]CHIVASA S,SIMON W J,MURPHY A M,et al.The effects of extracellular adenosine 5′-triphosphate on the tobacco proteome[J].Proteomics,2010,10(2):235-244.
[3]CHIVASA S,NDIMBA B K,Simon W J,et al.Extracellular ATP functions as an endogenous external metabolite regulating plant cell viability[J].The Plant Cell Online,2005,17(11):3 019-3 034.
[4]SONG C J,STEINEBRUNNER I,WANG X,et al.Extracellular ATP induces the accumulation of superoxide via NADPH oxidases in Arabidopsis[J].Plant Physiology,2006,140(4):1 222-1 232.
[5]DEMIDCHIK V,NICHOLS C,OLIYNYK M,et al.Is ATP a signaling agent in plants?[J].Plant Physiology,2003,133(2):456-461.
[6]DEMIDCHIK V,SHANG Z,SHIN R,et al.Plant extracellular ATP signalling by plasma membrane NADPH oxidase and Ca2+channels[J].The Plant Journal,2009,58(6):903-913.
[7]FORESI N P,LAXALT A M,TONN C V,et al.Extracellular ATP induces nitric oxide production in tomato cell suspensions[J].Plant Physiology,2007,145(3):589-592.
[8]SUN J,ZHANG C,ZHANG X,et al.Extracellular ATP signaling and homeostasis in plant cells[J].Plant Signaling&Behavior,2012,7(5):566-569.
[9]WOLF C,HENNIG M,ROMANOVICZ D,et al.Developmental defects and seedling lethality in apyrase AtAPY1and AtAPY2double knockout mutants[J].Plant Molecular Biology,2007,64(6):657-672.
[10]WU J,STEINEBRUNNER I,SUN Y,et al.Apyrases(nucleoside triphosphate-diphosphohydrolases)play a key role in growth control in Arabidopsis[J].Plant Physiology,2007,144(2):961-975.
[11]CHIVASA S,MURPHY A M,HAMILTON J M,et al.Extracellular ATP is a regulator of pathogen defence in plants[J].The Plant Journal,2009,60(3):436-448.
[12]RIEWE D,GROSMAN L,FERNIE A R,et al.The potato-specific apyrase is apoplastically localized and has influence on gene expression,growth,and development[J].Plant Physiology,2008,147(3):1 092-1 109.
[13]CLARK G,TORRES J,FINLAYSON S,et al.Apyrase(nucleoside triphosphate-diphosphohydrolase)and extracellular nucleotides regulate cotton fiber elongation in cultured ovules[J].Plant Physiology,2010,152(2):1 073-1 083.
[14]SUN J,ZHANG C L,DENG S R,et al.An ATP signalling pathway in plant cells:extracellular ATP triggers programmed cell death in Populus euphratica[J].Plant,Cell&Environment,2012,35(5):893-916.
[15]WIRTZ K W.Non-uniform scaling in phytoplankton growth rate due to intracellular light and CO2decline[J].Journal of Plankton Research,2011,33(9):1 325-1 341.
[16]HE J(何洁),LIU H X(刘鸿先),WANG Y R(王以柔),et al.Low temperature and photosynthesis of plants[J].Plant Physiology Communications(植物生理学通讯),1986,22(2):1-6(in Chinese).
[17]WU X X(吴雪霞),YANG X CH(杨晓春),ZHU Z W(朱宗文),et al.Effects of exogenous 6-BA on photosynthesis,chlorophyll fluorescence characteristics and the allocation of absorbed light in eggplant seedlings under low temperature stress[J].Plant Physiology Communications(植物生理学报),2013,49(11):1 181-1 188(in Chinese).
[18]DEMMIG-ADAMS B,ADAMS III W W,BARKER D H.Using chlorophyll fluorescence to assess the fraction of absorbed light allocated to thermal dissipation of excess excitation[J].Physiologia Plantarum,1996,98(2):253-264.
[19]SCHREIBER U,BILGER W,NEUBAUER C.Chlorophyll fluorescence as a nonintrusive indicator for rapid assessment of in vivo photosynthesis[M].Springer Berlin Heidelberg,1995:49-70.
[20]SONG L T(宋良图),SONG G L(宋国良),TAO H ZH(陶汉之).The primary study on cold tolerance of Brassica napus cultivars by measuring the electrical conductivity[J].Journal of Anhui Agricultural Sciences(安徽农业科学),1991,1:46-51(in Chinese).
[21]FUKUSHIMA E,ARATA Y,ENDO T,et al.Improved salt tolerance of transgenic tobacco expressing apoplastic yeast-derived invertase[J].Plant and Cell Physiology,2001,42(2):245-249.
[22]PERCIVAL G C,SHERIFFS C N.Identification of drought-tolerant woody periennials using chlorophyll fluorescence[J].Journal of Arboriculture,2002,28(5):215-223.
[23]ZHOU J(周建),YANG L F(杨立峰),HAO F G(郝峰鸽),et al.Photosynthesis and chlorophyll-fluorescence of Magnolia grandiflora seedlings under low temperature stress[J].Acta Bot.Boreal.-Occident.Sin.(西北植物学报),2009,29(1):136-142(in Chinese).
[24]GROOM Q J,BAKER N R.Analysis of light-induced depressions of photosynthesis in leaves of a wheat crop during the winter[J].Plant Physiology,1992,100:1 217-1 223.
[25]ANDREWS JR,FRYER MJ,BAKER NR.Characterization of chilling effects on photosynthetic performance of maize crops during early season growth using chlorophyll fluorescence[J].Journal of Experimental Botany,1995,46:1 195-1 203.
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[31]YOSHIDA K,TERASHIMA I,NOGUCHI K,et al..Distinct roles of the cytochrome pathway and alternative oxidase in leaf photosynthesis[J].Plant Cell Physiol.,2006,47:22-31.
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[33]TANAKA K,GILROY S,JONES A M,et al.Extracellular ATP signaling in plants[J].Trends in Cell Biology,2010,20(10):601-608.
[34]TANAKA K,SWANSON S J,GILROY S,et al.Extracellular nucleotides elicit cytosolic free calcium oscillations in Arabidopsis[J].Plant Physiology,2010,154(2):705-719.
[35]WANG W H,CHEN J,LIU T W,et al.Regulation of the calcium-sensing receptor in both stomatal movement and photosynthetic electron transport is crucial for water use efficiency and drought tolerance in Arabidopsis[J].Journal of Experimental Botany,2014,65(1):223-234.
[36]PETROUTSOS D,BUSCH A,JAN?EN I,et al.The chloroplast calcium sensor CAS is required for photoacclimation in Chlamydomonas reinhardtii[J].The Plant Cell,2011,23(8):2 950-2 963.
[37]LU X M(陆晓民),SUN J(孙锦),GUO SH R(郭世荣),et al.Effects of exogenous 24-epibrassinolide on the leaf photosynthetic characteristics and polyamines content of cucumber seedlings under hypoxia stress[J].Chinese Journal of Applied Ecology(应用生态学报),2012,23(1):140-146(in Chinese).