诱变剂对芦笋单倍体染色体加倍的比较
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摘要
[目的]建立芦笋单倍体染色体加倍的方法。[方法]以秋水仙碱与Oryzalin为诱变剂(均添加2%DMSO),在离体培养条件下,分别用浸泡法和培养基添加法处理芦笋单倍体材料的茎尖,通过传统的根尖压片法检测染色体数目,比较不同试剂、不同处理方法、不同浓度、不同处理时间下诱导二倍体的效果。[结果]秋水仙碱的处理效果优于试剂Oryzalin;培养基添加法的诱导效果优于浸泡法,在培养基中添加0.2%秋水仙素并处理5 d时诱导效果最佳,染色体加倍效率与成活率分别可达38.3%和50%。[结论]秋水仙碱仍是芦笋单倍体加倍最有效的诱变剂。
[Objective] To establish the method of doubling the haploid chromosome of asparagus. [Method]Under the conditions of in vitro culture,Colchicine and Oryzalin were used as mutants(both added 2 % DMSO). The stem tips of asparagus haploid materials were treated by soaking method and medium addition method, and the number of chromosomes was detected by the traditional root tip pressing method. Induced diploid effect of the different reagents, different treatment methods, different concentrations, different time were compared. [Result] Colchicine was better than the reagent Oryzalin. The induction effect of medium addition method was better than that of immersion method. When 0.2 % colchicine was added to the medium and treated for 5 days, the induction effect was best. The chromosome doubling frequency and survival rate could reach 38.3 % and 50 %, respectively. [Conclusion]Colchicine is still the most effective mutagenic agent for the haploid doubling of asparagus.
[Objective] To establish the method of doubling the haploid chromosome of asparagus. [Method]Under the conditions of in vitro culture,Colchicine and Oryzalin were used as mutants(both added 2 % DMSO). The stem tips of asparagus haploid materials were treated by soaking method and medium addition method, and the number of chromosomes was detected by the traditional root tip pressing method. Induced diploid effect of the different reagents, different treatment methods, different concentrations, different time were compared. [Result] Colchicine was better than the reagent Oryzalin. The induction effect of medium addition method was better than that of immersion method. When 0.2 % colchicine was added to the medium and treated for 5 days, the induction effect was best. The chromosome doubling frequency and survival rate could reach 38.3 % and 50 %, respectively. [Conclusion]Colchicine is still the most effective mutagenic agent for the haploid doubling of asparagus.
引文
[1] 李书华.芦笋标准化栽培技术[M].北京:中国农业出版社,2004:8-14,67-69.
[2] 叶劲松.芦笋的食疗与食谱[M].北京:台海出版社,2005:15-41.
[3] 张元国,于继庆,陈桂英,等.芦笋全雄株品种的培育途径及其关键技术[J].农业科技通讯,1997(6):16-17.
[4] 王小佳.蔬菜育种学(各论)[M].北京:中国农业出版社,2000:241-251.
[5] CHOO T M,REINBERGS E,KASHA K J.Use of haploids in barley breeding[J].Plant breeding reviews,1985,3:219-252.
[6] 周维燕.植物细胞工程原理与技术[M].北京:中国农业大学出版社,2001:127.
[7] HANSEN N J P,ANDERSEN S B.Efficient production of doubled haploid wheat plants by in vitro treatment of microspores with trifluralin or APM[J].Plants breeding,1998,117(5):401-405.
[8] 李静,李春明,卓泳杉,等.秋水仙碱诱导山杨异源多倍体[J].安徽农业科学,2014,42(4):1098-1100.
[9] VAN TUYL J M,MEIJER B,VAN DIEN M P.The use of Oryzalin as an alternative for Colchicine in in-vitro chromosome doubling of Lilium and Nerine[J].Acta horticulturae,1992,325:625-629.
[10] 朱惠琴.二甲基亚砜(DMSo)对烟草单倍体植株的染色体加倍效应[J].青海师范大学学报(自然科学版),2004(2):54-56.
[11] 张天翔,林宗铿,蔡坤秀,等.芦笋单倍体染色体加倍技术研究[J].中国农学通报,2011,27(13):212-215.
[12] TAKAMURA T, LIM K B, van TUYL J M.Effect of a new compounds on the mitotic polyploidization of Lilium longiflorum and oriental hybrid lilies[J].Acta horticulture,2002,572:37-42.
[2] 叶劲松.芦笋的食疗与食谱[M].北京:台海出版社,2005:15-41.
[3] 张元国,于继庆,陈桂英,等.芦笋全雄株品种的培育途径及其关键技术[J].农业科技通讯,1997(6):16-17.
[4] 王小佳.蔬菜育种学(各论)[M].北京:中国农业出版社,2000:241-251.
[5] CHOO T M,REINBERGS E,KASHA K J.Use of haploids in barley breeding[J].Plant breeding reviews,1985,3:219-252.
[6] 周维燕.植物细胞工程原理与技术[M].北京:中国农业大学出版社,2001:127.
[7] HANSEN N J P,ANDERSEN S B.Efficient production of doubled haploid wheat plants by in vitro treatment of microspores with trifluralin or APM[J].Plants breeding,1998,117(5):401-405.
[8] 李静,李春明,卓泳杉,等.秋水仙碱诱导山杨异源多倍体[J].安徽农业科学,2014,42(4):1098-1100.
[9] VAN TUYL J M,MEIJER B,VAN DIEN M P.The use of Oryzalin as an alternative for Colchicine in in-vitro chromosome doubling of Lilium and Nerine[J].Acta horticulturae,1992,325:625-629.
[10] 朱惠琴.二甲基亚砜(DMSo)对烟草单倍体植株的染色体加倍效应[J].青海师范大学学报(自然科学版),2004(2):54-56.
[11] 张天翔,林宗铿,蔡坤秀,等.芦笋单倍体染色体加倍技术研究[J].中国农学通报,2011,27(13):212-215.
[12] TAKAMURA T, LIM K B, van TUYL J M.Effect of a new compounds on the mitotic polyploidization of Lilium longiflorum and oriental hybrid lilies[J].Acta horticulture,2002,572:37-42.