采后苯并噻重氮处理对‘玉金香’甜瓜单萜类香气及其代谢关键酶的影响分析
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摘要
以‘玉金香’甜瓜(Cucumis melo L.)为实验材料,监测采后常温贮藏期间单萜类香气组分代谢l-脱氧-D-木酮糖-5-磷酸还原异构酶(l-deoxy-D-xylulose-5-phosphate reductoisomerase,DXR)活力及其单萜类香气组分的变化,探讨采后苯并噻重氮(benzo-(1,2,3)-thiadiazole-7-carbothioic acid S-methyl ester,BTH)诱抗处理对DXR活力和单萜类香气构成的影响,分析DXR活力与单萜类香气物质释放量的相关性。结果表明:贮藏期间,样品中共检出9种单萜类香气组分,其中8种单萜类香气物质含量与DXR活力呈正相关;采后BTH处理抑制了甜瓜DXR活力和单萜类香气物质的释放,延迟了DXR活力峰值出现,改变了单萜类香气释放规律及其释放量与DXR活力的相关性。与CC组(蒸馏水浸泡)相比,BTH处理组果皮、果肉样DXR活力分别降低了19.48%、25.49%;条件因子水可增大DXR活力峰值,但对DXR活力和单萜类香气的影响无明显规律。由此可见,采后BTH处理可通过抑制‘玉金香’甜瓜DXR活力,延迟活性峰值出现,改变单萜类香气释放量与DXR活力的相关性,影响单萜类香气的释放。
The objective of this study was to investigate the effect of postharvest treatment with the plant resistance inducer benzo(1,2,3)-thiadiazole-7-carbothioic acid S-methyl ester(BTH) on the activity of 1-deoxy-D-xylulose-5-phosphate reductoisomerase(DXR) and aroma-active monoterpenes in muskmelon(Cucumis melo L. cv. Yujinxiang) and to analyze the correlation between DXR activity and the release of aroma-active monoterpenes. Changes in DXR activity, involved in the metabolic pathway of aroma-active monoterpenes and monoterpene composition were monitored during postharvest storage at room temperature. The results showed that a total of nine aroma-active monoterpene components were detected in the samples during the storage period. Eight of these compounds were positively correlated with DXR activity. Upon postharvest BTH treatment, DXR activity and the release of monoterpenes were inhibited accompanied by delayed peak time of DXR activity as well as altered release pattern of aroma-active monoterpenes and correlation with DXR activity. Compared with the CC group(distilled water immersion), DXR activity in the rind and pulp of BTH-treated melon was reduced by 19.48% and 25.49%, respectively. The effect of distilled water immersion on DXR activity and the release of aroma-active monoterpenes was irregular although it increased the peak of DXR activity. From these findings, it is clear that postharvest BTH treatment can inhibit DXR activity, delay the peak time of DXR activity and affect the release of aromaactive monoterpenes and its correlation with DXR activity.
The objective of this study was to investigate the effect of postharvest treatment with the plant resistance inducer benzo(1,2,3)-thiadiazole-7-carbothioic acid S-methyl ester(BTH) on the activity of 1-deoxy-D-xylulose-5-phosphate reductoisomerase(DXR) and aroma-active monoterpenes in muskmelon(Cucumis melo L. cv. Yujinxiang) and to analyze the correlation between DXR activity and the release of aroma-active monoterpenes. Changes in DXR activity, involved in the metabolic pathway of aroma-active monoterpenes and monoterpene composition were monitored during postharvest storage at room temperature. The results showed that a total of nine aroma-active monoterpene components were detected in the samples during the storage period. Eight of these compounds were positively correlated with DXR activity. Upon postharvest BTH treatment, DXR activity and the release of monoterpenes were inhibited accompanied by delayed peak time of DXR activity as well as altered release pattern of aroma-active monoterpenes and correlation with DXR activity. Compared with the CC group(distilled water immersion), DXR activity in the rind and pulp of BTH-treated melon was reduced by 19.48% and 25.49%, respectively. The effect of distilled water immersion on DXR activity and the release of aroma-active monoterpenes was irregular although it increased the peak of DXR activity. From these findings, it is clear that postharvest BTH treatment can inhibit DXR activity, delay the peak time of DXR activity and affect the release of aromaactive monoterpenes and its correlation with DXR activity.
引文
[1]徐涛,蒋玉梅,李霁昕,等.采后BTH处理对“玉金香”厚皮甜瓜生理特性及感官品质的影响[J].食品工业科技, 2014, 35(11): 315-318.DOI:10.13386/j.issn1002-0306.2014.11.061.
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[3]徐兰英,汪跃华,庞学群,等.BTH和BABA处理对采后沙糖桔病害和品质的影响[J].广东农业科学, 2010, 37(8): 256-258. DOI:10.3969/j.issn.1004-874X.2010.08.103.
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[6]邬子燕,韩育梅.甜瓜风味物质的研究进展[J].保鲜与加工,2010,10(6): 51-53.DOI:10.3969/j.issn.1009-6221.2010.06.014.
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[9]张娜.采后BTH处理对“玉金香”甜瓜特征香气及LOX酶活性的影响[D].兰州: 甘肃农业大学, 2014: 35.
[10]王博,李霁昕,李经纬,等.采后苯并噻重氮处理对‘玉金香’甜瓜氨基酸代谢酯类香气物质及其代谢机理的影响[J].食品科学,2018,39(17): 212-220. DOI:10.7506/spkx1002-6630-201817035.
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[12]孙晋.番茄萜类化合物生物合成途径相关酶基因的克隆与分析[D].武汉: 华中农业大学, 2008: 6-8. DOI:10.7666/d.Y1659568.
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[14]张冬桃,孙君,叶乃兴,等.茶树萜烯类香气物质合成相关酶研究进展[J].茶叶学报, 2015, 56(2): 68-79.DOI:10.3969/j.issn.1007-4872.2015.02.002.
[15] VIEIRA DOS SANTOS M C, CURTIS R H C, ABRANTES I, et al. Effect of plant elicitors on the reproduction of the root-knot nematode Meloidogyne chitwoodi on susceptible hosts[J]. European Journal Plant Pathology, 2013. 136(1): 193-202.DOI:10.1007/s10658-012-0155-6.
[16] BI Yang, GE Yonghong, LI Yongcai. Postharvest acibenzolar-S-methyl treatment suppresses decay and induces resistance in Hami melon[J]. Acta Hoculturae, 2006, 712(1): 393-399.DOI:10.17660/ActaHortic.2006.712.45.
[17] ZHANG Bo, YIN Xueren, LI Xian, et al. Lipoxygenase gene expression in ripening kiwifruit in relation to ethylene and aroma production[J]. Journal of Agricultural and Food Chemistry, 2009, 57(7): 2875-2881. DOI:10.1021/jf9000378.
[18]HASUNUMA T,TAKENO S,HAYASHI S,et al.Overexpression of1-deoxy-D-xylulose-5-phosphate reductoisomerase gene in chloroplast contributes to increment of isoprenoid production[J].Bioscience and Bioengineering,2008,105(5):518-526.DOI:10.1263/jbb.105.518.
[19]徐涛.采后ASM处理对“玉金香”甜瓜乙烯代谢及挥发性物质释放的影响[D].兰州:甘肃农业大学,2014:16.
[20]刘宇.过表达苹果α-法尼烯合酶基因烟草早衰机制研究[D].泰安:山东农业大学,2016:11.
[21]MUNOZ-BERTOMEU J,ARRILLAGA I,ROS R,et al.Up-regulation of 1-deoxy-D-xylulose-5-phosphate synthase enhances production of essential oils in transgenic spike lavender[J]. Plant Physiology, 2006, 142(3): 890-900.DOI:10.1104/pp.106.086355.
[22]WU S J,SHI M,WU J Y.Cloning and characterization of the 1-deoxyD-xylulose 5-phosphate reductoisomerase gene for diterpenoid tanshinone biosynthesis in Salvia miltiorrhiza(Chinese sage)hairy roots[J].Biotechnology Applied Biochemistry,2009,52(1):89-95.DOI:10.1042/BA20080004.
[23]李恒.1.萜类化合物MEP生物合成途径中dxr基因的克隆表达及酶活性分析2.野芝麻中环烯醚萜苷的生物合成研究[D].西安: 西北大学, 2009: 11. DOI:10.7666/d.y1454054.
[24] MAHMOUD S S, CROTEAU R B. Metabolic engineering of essential oil yield and composition in mint by altering expression of deoxyxylulose phosphate reductoisomerase and menthofuran synthase[J]. Proceeds of National Academy of Science, 2001, 98(15): 8915-8920.DOI:10.1073 /pnas.141237298.
[25]王旭日.姜花DXS、DXR基因克隆及萜类香气代谢途径研究[D].广州: 华南农业大学, 2008: 47-49. DOI:10.7666/d.Y1278020.
[26]王伟.低醇起泡葡萄酒风味物质的研究[D].天津: 天津大学, 2011: 3-4.
[27]孙磊,朱保庆,王晓玥,等.早中熟鲜食葡萄5个品种及其亲本果实单萜成分分析[J].园艺学报, 2016, 43(11): 2109-2118.DOI:10.16420/j.issn.0513-353x.2016-0164.
[28]吴勇.萜烯类化合物与茶叶香气[J].化学工程与装备, 2009(11): 123-125.DOI:10.3969/j.issn.1003-0735.2009.11.042.
[29]涂崔,潘秋红,朱保庆,等.葡萄与葡萄酒单萜化合物的研究进展[J].园艺学报, 2011, 38(7): 1397-1406.DOI:10.16420/j.issn.0513-353x.2011.07.025.
[30]RIBéREAU-GAYON P,GLORIES Y,MAJUEAN A,et al.Handbook of enology:the chemistry of wine stabilization and treatments[M].2nd.New York:John Wiley&Sons,2006:112-116.DOI:10.1002/0470010398.ch5.
[2]刘芳,董生俊,陈年来.贮藏温度对甜瓜果实采后病害和生理代谢的影响[J].食品科技, 2005, 30(1): 84-87.DOI:10.3969/j.issn.1005-9989.2005.01.028.
[3]徐兰英,汪跃华,庞学群,等.BTH和BABA处理对采后沙糖桔病害和品质的影响[J].广东农业科学, 2010, 37(8): 256-258. DOI:10.3969/j.issn.1004-874X.2010.08.103.
[4] GE Y H, BI Y, LI X, et al. Induces resistance against fusarium and pink rots by acibenzolar-S-methyl in harvested muskmelon (cv. Yindi)[J]. Agricultural Sciences in China, 2008, 7(1): 58-64.DOI:10.1016/S1671-2927(08)60022-X.
[5]任亚琳,毕阳,葛永红,等.BTH浸泡处理对厚皮甜瓜采后病害的控制及贮藏品质的影响[J].食品科学, 2013, 34 (2): 267-272.
[6]邬子燕,韩育梅.甜瓜风味物质的研究进展[J].保鲜与加工,2010,10(6): 51-53.DOI:10.3969/j.issn.1009-6221.2010.06.014.
[7] AUBERT C, BOURGER N. Investigation of volatiles in Charentais cantaloupe melons (Cucumis melo Var.cantalupensis). characterization of aroma constituents in some cultivars[J]. Journal Agricultural and Food Chemistry, 2004, 52(14): 4522-4528.DOI:10.1021/jf049777s.
[8] DIXON J, HEWETT E W. Factors affecting apple aroma/flavour volatile concentration: a review[J]. New Zealand Journal of Crop and Horticultural Science, 2000, 28(3): 155-173.DOI:10.1080/01140671.2000.9514136.
[9]张娜.采后BTH处理对“玉金香”甜瓜特征香气及LOX酶活性的影响[D].兰州: 甘肃农业大学, 2014: 35.
[10]王博,李霁昕,李经纬,等.采后苯并噻重氮处理对‘玉金香’甜瓜氨基酸代谢酯类香气物质及其代谢机理的影响[J].食品科学,2018,39(17): 212-220. DOI:10.7506/spkx1002-6630-201817035.
[11] BOHLMANN J, KEELING C I. Terpenoid biomaterials[J]. Plant Journal, 2008, 54(4): 656-669.DOI:10.1111/j.1365-313X.2008.03449.
[12]孙晋.番茄萜类化合物生物合成途径相关酶基因的克隆与分析[D].武汉: 华中农业大学, 2008: 6-8. DOI:10.7666/d.Y1659568.
[13] ESTéVEZ J M, CANTERO A, REINDL A, et al. 1-deoxy-D-xylulose-5-phosphate synthase, a limiting enzyme for plastidic isoprenoid biosynthesis in plants[J]. Journal of Biological Chemistry, 2001, 276(25): 22901-22909.DOI:10.1074/jbc.M100854200.
[14]张冬桃,孙君,叶乃兴,等.茶树萜烯类香气物质合成相关酶研究进展[J].茶叶学报, 2015, 56(2): 68-79.DOI:10.3969/j.issn.1007-4872.2015.02.002.
[15] VIEIRA DOS SANTOS M C, CURTIS R H C, ABRANTES I, et al. Effect of plant elicitors on the reproduction of the root-knot nematode Meloidogyne chitwoodi on susceptible hosts[J]. European Journal Plant Pathology, 2013. 136(1): 193-202.DOI:10.1007/s10658-012-0155-6.
[16] BI Yang, GE Yonghong, LI Yongcai. Postharvest acibenzolar-S-methyl treatment suppresses decay and induces resistance in Hami melon[J]. Acta Hoculturae, 2006, 712(1): 393-399.DOI:10.17660/ActaHortic.2006.712.45.
[17] ZHANG Bo, YIN Xueren, LI Xian, et al. Lipoxygenase gene expression in ripening kiwifruit in relation to ethylene and aroma production[J]. Journal of Agricultural and Food Chemistry, 2009, 57(7): 2875-2881. DOI:10.1021/jf9000378.
[18]HASUNUMA T,TAKENO S,HAYASHI S,et al.Overexpression of1-deoxy-D-xylulose-5-phosphate reductoisomerase gene in chloroplast contributes to increment of isoprenoid production[J].Bioscience and Bioengineering,2008,105(5):518-526.DOI:10.1263/jbb.105.518.
[19]徐涛.采后ASM处理对“玉金香”甜瓜乙烯代谢及挥发性物质释放的影响[D].兰州:甘肃农业大学,2014:16.
[20]刘宇.过表达苹果α-法尼烯合酶基因烟草早衰机制研究[D].泰安:山东农业大学,2016:11.
[21]MUNOZ-BERTOMEU J,ARRILLAGA I,ROS R,et al.Up-regulation of 1-deoxy-D-xylulose-5-phosphate synthase enhances production of essential oils in transgenic spike lavender[J]. Plant Physiology, 2006, 142(3): 890-900.DOI:10.1104/pp.106.086355.
[22]WU S J,SHI M,WU J Y.Cloning and characterization of the 1-deoxyD-xylulose 5-phosphate reductoisomerase gene for diterpenoid tanshinone biosynthesis in Salvia miltiorrhiza(Chinese sage)hairy roots[J].Biotechnology Applied Biochemistry,2009,52(1):89-95.DOI:10.1042/BA20080004.
[23]李恒.1.萜类化合物MEP生物合成途径中dxr基因的克隆表达及酶活性分析2.野芝麻中环烯醚萜苷的生物合成研究[D].西安: 西北大学, 2009: 11. DOI:10.7666/d.y1454054.
[24] MAHMOUD S S, CROTEAU R B. Metabolic engineering of essential oil yield and composition in mint by altering expression of deoxyxylulose phosphate reductoisomerase and menthofuran synthase[J]. Proceeds of National Academy of Science, 2001, 98(15): 8915-8920.DOI:10.1073 /pnas.141237298.
[25]王旭日.姜花DXS、DXR基因克隆及萜类香气代谢途径研究[D].广州: 华南农业大学, 2008: 47-49. DOI:10.7666/d.Y1278020.
[26]王伟.低醇起泡葡萄酒风味物质的研究[D].天津: 天津大学, 2011: 3-4.
[27]孙磊,朱保庆,王晓玥,等.早中熟鲜食葡萄5个品种及其亲本果实单萜成分分析[J].园艺学报, 2016, 43(11): 2109-2118.DOI:10.16420/j.issn.0513-353x.2016-0164.
[28]吴勇.萜烯类化合物与茶叶香气[J].化学工程与装备, 2009(11): 123-125.DOI:10.3969/j.issn.1003-0735.2009.11.042.
[29]涂崔,潘秋红,朱保庆,等.葡萄与葡萄酒单萜化合物的研究进展[J].园艺学报, 2011, 38(7): 1397-1406.DOI:10.16420/j.issn.0513-353x.2011.07.025.
[30]RIBéREAU-GAYON P,GLORIES Y,MAJUEAN A,et al.Handbook of enology:the chemistry of wine stabilization and treatments[M].2nd.New York:John Wiley&Sons,2006:112-116.DOI:10.1002/0470010398.ch5.