摘要
目的了解淮安市一游泳池聚集性感染人腺病毒的hexon基因特征。方法采用实时荧光定量RT-PCR方法对12例游泳后出现呼吸道感染患儿咽拭子标本和2份游泳池水样进行呼吸道多病原体的快速筛查,对核酸阳性的标本进行hexon基因的部分序列扩增、测序并构建系统发生树,分析hexon基因特征及人腺病毒遗传多样性确定腺病毒的型别。结果 12例患儿中有9份咽拭子标本和1份儿童池游泳水样扩增出人腺病毒hexon基因片段,测序分析该毒株(淮安株)与我国GZ01株、日本J1007、美国株(USA27440、USA 5497、USA NHR3)核苷酸同源性及推导的氨基酸同源性均为99.9%。系统进化树分析显示,淮安株属于人腺病毒E亚属4基因型的进化谱系枝。结论该起聚集性呼吸道感染疫情可能由E亚属4基因型人腺病毒污染游泳池水引起。
Objective To study the genetic characteristics of adenovirus from clustered human cases occurring at a municipal swimming pool. Methods Nasopharyngeal swabs were collected from 12 pediatric patients who developed an upper respiratory tract infection after swimming, and 2 samples of pool water were collected. Samples were screened for multiple respiratory pathogens using quantitative real-time RT-PCR. The genetic characteristics of and the phylogenetic relationship among human adenoviruses were determined by analyzing the sequence of the hexon gene. Results Human adenovirus nucleic acids were detected in 9 of 12 nasopharyngeal swabs and one sample of pool water. Comparison indicated that the Huai'an strains were very closely related to GZ01, J1007, USA27440, USA 5497, USA NHR3. Nucleotide sequences were 99.9% similar, and deduced amino acid sequences were 99.9% similar. A phylogenetic tree indicated the Huai'an strains clustered together and belonged to a genetic lineage of HAdV-4 within the E species. Conclusion Swimming pool water contaminated with HAdV-4 was the most likely source of infection.
引文
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