模拟破坏苦杏仁和桃仁品质及酶活性变化研究
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摘要
目的探讨贮存过程中苦杏仁和桃仁品质的变化规律。方法在温度为35℃、相对湿度为80%的环境中放置1,3,4 d,测定油脂含量、酸价、过氧化值、β-葡萄糖苷酶和多酚氧化酶活性。结果放置4 d后,苦杏仁、桃仁油脂含量分别从原来的59%降至47%和37%;油脂氧化程度不断升高;苦杏仁和桃仁的苦杏仁苷含量分别从67,58 mg/g降至55,55 mg/g;β-葡萄糖苷酶和多酚氧化酶活力均呈上升趋势。结论模拟苦杏仁和桃仁实际贮存条件的加速破坏试验,可为探讨其品质的变化规律和变质预测提供参考,从控制酶的活性来保障苦杏仁、桃仁和其他富油果仁贮存品质值得进一步研究。
Objective To investigate the quality changes law of Armeniacae Semen Amarum and Persicae Semen during the process of storage. Methods The lipid content,acid value,peroxide value,β-glucosidase and polyphenol oxidase activity were determined after 1,3 and 4 d of storage at 35 ℃ and 80% relative humidity. Results After 4 d of storage,the oil content of Armeniacae Semen Amarum and Persicae Semen decreased from 59% to 47% and 37% respectively,the degree of oil oxidation increased continuously. The content of amygdalin decreased from 67 mg/g to 55 mg/g in Armeniacae Semen Amarum,and from 58 mg/g to 55 mg/g in Persicae Semen.The activity of β-glucosidase and polyphenol oxidase both showed an upward trend. Conclusion The accelerated damage test simulated the actual storage conditions of Armeniacae Semen Amarum and Persicae Semen can provide reference for investigating the quality change law and the deterioration prediction of Armeniacae Semen Amarum and Persicae Semen. It is worthwhile to further study how to control the activity of enzymes to ensure the storage quality of Armeniacae Semen Amarum and Persicae Semen and other oil-rich kernels.
Objective To investigate the quality changes law of Armeniacae Semen Amarum and Persicae Semen during the process of storage. Methods The lipid content,acid value,peroxide value,β-glucosidase and polyphenol oxidase activity were determined after 1,3 and 4 d of storage at 35 ℃ and 80% relative humidity. Results After 4 d of storage,the oil content of Armeniacae Semen Amarum and Persicae Semen decreased from 59% to 47% and 37% respectively,the degree of oil oxidation increased continuously. The content of amygdalin decreased from 67 mg/g to 55 mg/g in Armeniacae Semen Amarum,and from 58 mg/g to 55 mg/g in Persicae Semen.The activity of β-glucosidase and polyphenol oxidase both showed an upward trend. Conclusion The accelerated damage test simulated the actual storage conditions of Armeniacae Semen Amarum and Persicae Semen can provide reference for investigating the quality change law and the deterioration prediction of Armeniacae Semen Amarum and Persicae Semen. It is worthwhile to further study how to control the activity of enzymes to ensure the storage quality of Armeniacae Semen Amarum and Persicae Semen and other oil-rich kernels.
引文
[1]颜永刚,裴瑾,杨新杰,等.中药桃仁的品种、品质与药效相关性分析研究[J].成都医学院学报,2011,6(4):296-298.
[2]国家药典委员会.中华人民共和国药典[M].北京:中国医药科技出版社,2015:201.
[3]阎敏,廖建萍.贮存条件对苦杏仁质量的影响[J].临床药理学,2009,20(1):72-74.
[4]赵素莲,刘桂芬,戴兆雄,等.苦杏仁甙对小鼠免疫功能的影响[J].山西医药杂志,1993,22(3):166.
[5]赵素莲,戴兆雄,李春华.苦杏仁甙对小鼠脾脏NK细胞活性的影响[J].山西医科大学学报,1993,24(1):14-16.
[6]李春,童德中,解方,等.苦杏仁甙对小鼠肝枯否细胞r DNA活性及吞噬功能的影响[J].实用中西医结合杂志,1994(1):7-8.
[7]王芳,李,薛鑫,等.初匀速法预测制川乌的有效期[J].中国实验方剂学杂志,2014,20(10):12-15.
[8]周大鹏,薛志平,刘杰,等.经典恒温法预测杭白菊和贡菊的有效期[J].中国中药杂志,2012,37(21):3182.
[9]王金梅,姚辰,李昌勤,等.经典恒温法预测红花的有效期[J].天然产物研究与开发,2017(2):273-277.
[10]王金梅,李昌勤,康文艺.初匀速法测定中药薄荷有效期[J].中国中药杂志,2012,37(21):3185.
[11]拱健婷.易变质中药加速试验方法研究与质量预测模型的建立[D].北京:北京中医药大学,2017.
[12]颜永刚.桃仁质量研究[D].成都:成都中医药大学,2008.
[13]张清安,范学辉,武海波,等.苦杏仁多酚氧化酶的理化特性[J].食品科学,2014,35(15):132-135.
[14]赵丽莹,拱健婷,米文娟,等.苦杏仁中β-葡萄糖苷酶活性测定条件的研究[J].中南药学,2017,15(2):166-169.
[15]房敏峰,付志玲,王启林,等.炮制对苦杏仁中苦杏仁苷在大鼠体内代谢的影响[J].中国中药杂志,2010,35(20):2684-2688.
[16]申辉,张清安,张馨允,等.苦杏仁多酚氧化酶的分离纯化及部分特性分析[J].中国油脂,2017,42(9):136-140.
[2]国家药典委员会.中华人民共和国药典[M].北京:中国医药科技出版社,2015:201.
[3]阎敏,廖建萍.贮存条件对苦杏仁质量的影响[J].临床药理学,2009,20(1):72-74.
[4]赵素莲,刘桂芬,戴兆雄,等.苦杏仁甙对小鼠免疫功能的影响[J].山西医药杂志,1993,22(3):166.
[5]赵素莲,戴兆雄,李春华.苦杏仁甙对小鼠脾脏NK细胞活性的影响[J].山西医科大学学报,1993,24(1):14-16.
[6]李春,童德中,解方,等.苦杏仁甙对小鼠肝枯否细胞r DNA活性及吞噬功能的影响[J].实用中西医结合杂志,1994(1):7-8.
[7]王芳,李,薛鑫,等.初匀速法预测制川乌的有效期[J].中国实验方剂学杂志,2014,20(10):12-15.
[8]周大鹏,薛志平,刘杰,等.经典恒温法预测杭白菊和贡菊的有效期[J].中国中药杂志,2012,37(21):3182.
[9]王金梅,姚辰,李昌勤,等.经典恒温法预测红花的有效期[J].天然产物研究与开发,2017(2):273-277.
[10]王金梅,李昌勤,康文艺.初匀速法测定中药薄荷有效期[J].中国中药杂志,2012,37(21):3185.
[11]拱健婷.易变质中药加速试验方法研究与质量预测模型的建立[D].北京:北京中医药大学,2017.
[12]颜永刚.桃仁质量研究[D].成都:成都中医药大学,2008.
[13]张清安,范学辉,武海波,等.苦杏仁多酚氧化酶的理化特性[J].食品科学,2014,35(15):132-135.
[14]赵丽莹,拱健婷,米文娟,等.苦杏仁中β-葡萄糖苷酶活性测定条件的研究[J].中南药学,2017,15(2):166-169.
[15]房敏峰,付志玲,王启林,等.炮制对苦杏仁中苦杏仁苷在大鼠体内代谢的影响[J].中国中药杂志,2010,35(20):2684-2688.
[16]申辉,张清安,张馨允,等.苦杏仁多酚氧化酶的分离纯化及部分特性分析[J].中国油脂,2017,42(9):136-140.