葫芦巴超临界CO_2提取物的GC-MS分析及对DPPH自由基的清除作用
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摘要
采用超临界二氧化碳提取葫芦巴提取物,利用气相色谱-质谱法分析提取物的化学成分,并通过面积归一化法分析各成分的相对含量。从葫芦巴超临界二氧化碳提取物中共鉴定出49个化合物,占提取物总量的88.37%,其中亚油酸(45.29%)含量最高。利用分光光度法测定葫芦巴超临界二氧化碳提取物对DPPH自由基的清除作用,其清除作用良好,半清除浓度为4.57 mg·m L-1,且清除率与样品浓度呈量效关系。葫芦巴超临界提取物营养成分丰富、安全无毒、有特殊香气,在食品、香料等领域可广泛应用。
Supercritical fluid extraction( SFE) using carbon dioxide was employed to extract Trigonella foenum-graecum L.and the chemical components were determined by GC- MS. The relative contents of the chemical components were determined with peak area normalization method. Forty- nine compounds,which occupied88.37% of total constituents were identified. The major constituent was linoleic acid( 45.29%). The scavenging capacity against DPPH free radical of SFE extracts from Trigonella foenum- graecum L.was determined by spectro photometry.The extracts exhibited significant scavenging capacity against DPPH free radical in a concentrationdependent fashion with the SC50 value of 4.57 mg·m L- 1.The SFE extracts was rich in nutrients,safe and non- toxic,and had special aroma.So it could be widely used in the fields of food and perfume.
Supercritical fluid extraction( SFE) using carbon dioxide was employed to extract Trigonella foenum-graecum L.and the chemical components were determined by GC- MS. The relative contents of the chemical components were determined with peak area normalization method. Forty- nine compounds,which occupied88.37% of total constituents were identified. The major constituent was linoleic acid( 45.29%). The scavenging capacity against DPPH free radical of SFE extracts from Trigonella foenum- graecum L.was determined by spectro photometry.The extracts exhibited significant scavenging capacity against DPPH free radical in a concentrationdependent fashion with the SC50 value of 4.57 mg·m L- 1.The SFE extracts was rich in nutrients,safe and non- toxic,and had special aroma.So it could be widely used in the fields of food and perfume.
引文
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[14]王雅,王玉丽,赵萍,等.超声波辅助提取葫芦巴香精油工艺优化及其抑菌性能研究[J].食品与发酵工业,2010,36(5):170-173.
[15]金靚婕,朱世云,梁成红,等.超临界CO2提取辣椒油树脂的工艺研究[J].食品工业科技,2012,33(5):185-189.
[16]Zhenchun Chen,Xin Mei,Yuxia Jin,et al.Optimisation of supercritical carbon dioxide extraction of essential oil of flowers of tea(Camellia sinensis L.)plants and its antioxidative activity[J].Journal of the Science of Food and Agriculture,2014,94:316-321.
[17]孙强伟,胡春芹,齐应才,等.啤酒糟挥发油化学成分的GC-MS分析及对DPPH自由基的清除作用[J].食品工业科技,2014,35(1):49-52.
[18]Awika JM,Rooney LW,Wu X L,et al.Screening methods to measure antioxidant activity of sorghum(Sorghum bicolor)and sorghum products[J].Journal of Agricultural and Food Chemistry,2003,51(23):6657-6662.
[2]LU FR,SHEN L,QIN Y,et al.Clinica1 observation on Trigonella foenum-graecum L total saponins in combinaiton with sulphanylureas in the treatment of type 2 diabetes mellitus[J].Chin J Integr Med,2008,14(1):56-60.
[3]THAKRAN S,SIDDIQUI MR,BAQUER NZ.Trigonella foenum-graecum seed powder protects against histopathological abnormalities in tissues of diabetic rats[J].Mol Cell Biochem,2004,266(1-2):151-159.
[4]卢芙蓉,秦铀,沈霖,等.葫芦巴提取物对肾病综合征大鼠肾损伤的保护作用[J].中西医结合研究,2009,1(6):281-284.
[5]于忠香.芳香特菜葫芦巴[J].北京农业,2003(10):4.
[6]周淑晶.中药葫芦巴的化学成分研究进展[J].中药研究与信息,2000,2(5):19-22.
[7]黄伟.SDE/GC-MS法对两种不同葫芦巴浸膏香气成分的分析[J].上海应用技术学院学报,2005,5(4):318-321.
[8]姚健,马君义,张继,等.发酵对葫芦巴挥发性化学成分的影响[J].食品科学,2006,27(12):194-198.
[9]Moniruzzaman,Shahinuzzaman,Ahsanul Haque,et al.Gas chromatography mass spectrometry analysis and in vitro antibacterial activity of essential oil from Trigonella foenumgraecum[J].Asian Pacific Journal of Tropical Biomedicine,2015,5(12):1033-1036.
[10]刘世巍,黄述州,丁建海.葫芦巴挥发油成分的GC-MS分析[J].华西药学杂志,2013,28(5):504-505.
[11]董丽,蔡凌霜,朱书奎,等.固相微萃取与气相色谱/质谱法联用分析葫芦巴浸膏的挥发性成分[J].武汉大学学报(理学版),2004,50(2):151-156.
[12]苏勇,冒德寿,曲荣芬,等.搅拌棒磁子萃取-热脱附-气相色谱质谱联用分析葫芦巴浸膏的挥发性成分[J].食品工业科技,2012,33(13):78-81.
[13]苏勇,冒德寿,曲荣芬,等.搅拌棒磁子萃取-热脱附-气相色谱质谱联用分析葫芦巴酊剂的的挥发性成分[J].香料香妆化学品,2012,40(4):5-10.
[14]王雅,王玉丽,赵萍,等.超声波辅助提取葫芦巴香精油工艺优化及其抑菌性能研究[J].食品与发酵工业,2010,36(5):170-173.
[15]金靚婕,朱世云,梁成红,等.超临界CO2提取辣椒油树脂的工艺研究[J].食品工业科技,2012,33(5):185-189.
[16]Zhenchun Chen,Xin Mei,Yuxia Jin,et al.Optimisation of supercritical carbon dioxide extraction of essential oil of flowers of tea(Camellia sinensis L.)plants and its antioxidative activity[J].Journal of the Science of Food and Agriculture,2014,94:316-321.
[17]孙强伟,胡春芹,齐应才,等.啤酒糟挥发油化学成分的GC-MS分析及对DPPH自由基的清除作用[J].食品工业科技,2014,35(1):49-52.
[18]Awika JM,Rooney LW,Wu X L,et al.Screening methods to measure antioxidant activity of sorghum(Sorghum bicolor)and sorghum products[J].Journal of Agricultural and Food Chemistry,2003,51(23):6657-6662.