不同浓度杨梅树皮浸提液对白菜种子的化感效应
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摘要
以白菜种子为受体,采用生物测定的方法,研究了杨梅树皮化感效应。结果表明,不同浓度杨梅树皮浸提液对白菜种子的发芽势、发芽指数表现为抑制作用,且浓度越高抑制作用越明显,在发芽率和活力指数方面呈现出"低促高抑"现象,当浓度为0.4 g·m L~(-1)时,促进作用转变为抑制作用;对白菜幼苗的茎生长表现出促进作用,且低浓度促进作用显著,对根生长表现为抑制作用,且高浓度抑制作用显著。综上说明,杨梅树皮浸提液中存在化感物质,且其化感物质对白菜各项生理指标的化感效应和作用强度均不同。
The allelopathic effects of extracts from the bark of Myrica rubra on cabbage seeds were studied by adopting the bioassay method. The results showed that different concentrations of extract liquid of Myrica rubra bark had a negative effect on germination potential and germination index of cabbage seeds and the higher the concentration was the stronger the inhibition became.It also had effect on the germination rate and vigor index and the effect showed the"Hormesis effect",when the concentration reached 0.4 g·m L~(-1),the promotion was changed into inhibition.It had a positive effect on the growth of stem and the promoting effect was conspicuous.It had a negative effect on the growth of root and the inhibiting effect of the high concentration was conspicuous.In conclusion,there were some allelochemicals that had allelopathic influence and different strength of the physiological indexes of cabbage seeds.
The allelopathic effects of extracts from the bark of Myrica rubra on cabbage seeds were studied by adopting the bioassay method. The results showed that different concentrations of extract liquid of Myrica rubra bark had a negative effect on germination potential and germination index of cabbage seeds and the higher the concentration was the stronger the inhibition became.It also had effect on the germination rate and vigor index and the effect showed the"Hormesis effect",when the concentration reached 0.4 g·m L~(-1),the promotion was changed into inhibition.It had a positive effect on the growth of stem and the promoting effect was conspicuous.It had a negative effect on the growth of root and the inhibiting effect of the high concentration was conspicuous.In conclusion,there were some allelochemicals that had allelopathic influence and different strength of the physiological indexes of cabbage seeds.
引文
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[23]王晓英,冯殿齐,张元湖,等.古树对萝卜、白菜化感作用的生物测定和作用机制研究[J].山东农业大学学报:自然科学版,2013,44(3):333-339.
[24]胡远彬.茎直黄芪的化感作用及抗氧化性研究[D].杨凌:西北农林科技大学,2013.
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[26]夏其乐,陈健初,吴丹.杨梅叶提取物抗氧化活性的研究[J].食品科学,2004,25(8):80-83.
[27]董悦涵.杨梅树皮提取物中总黄酮快速分析方法研究[J].南方农业学报,2011,42(10):1263-1266.
[28]李国成,陈楚雄,罗嘉玲,等.杨梅叶降血糖有效部位的化学成分研究[J].中草药,2011,42(5):863-865.
[29]谢苑,高素萍.白三叶水浸液对高羊茅种子萌发及幼苗生长的影响[J].安徽农业科学,2008,36(9):3616-3618.
[30]杜吉到,屈春媛,李明,等.PEG模拟干旱胁迫对芸豆萌发的影响[J].黑龙江八一农垦大学学报,2015,26(5):1-5.
[31]Pandey D K,Kauraw L P,Bhan V M.Inhibitory effect of parthenium(Parthenium hysterophorus L.)residue on growth of water hyacinth(Eichhornia crassipes mart solms).Ⅰ.Effect of leaf residue[J].Journal of Chemical Ecology,1993,19:2651-2662.
[2]李三玉,李兴军,胡东维,等.杨梅花芽发端期间芽体和叶肉细胞内钙调素的分布[J].科技通报,2002,18(2):137-141.
[3]中国科学院中国植物志编辑委员会.中国植物志:(第二十一卷)[M].北京:科学出版社,1979.
[4]熊文愈.林农复合生态系统学术讨论会论文集(林农复合生态系统的类型和效益)[M].哈尔滨:东北林业大学出版社,1988.
[5]郭兰萍,黄璐琦,蒋有绪,等.苍术根茎及根际土水提物生物活性研究及化感物质的鉴定[J].生态学报,2006,26(2):528-535.
[6]王大力.水稻化感作用研究综述[J].生态学报,1998,18(3):104-112.
[7]阎飞,杨振明,韩丽梅.植物化感作用(Allelopathy)及其作用物的研究方法[J].生态学报,2000,20(4):692-696.
[8]田楠,刘增文,李俊,等.林(果)粮间作中树木枯落叶对小麦发芽期和苗期的化感效应[J].中国生态农业学报,2013,21(6):707-714.
[9]由海霞,梁银丽,吕文,等.不同作物根系分泌物对黄瓜的化感作用[J].西北农林科技大学学报:自然科学版,2006,34(6):101-105.
[10]刘传菊.杨梅中酚类物质的分离、分析、特性及应用研究[D].武汉:华中农业大学,2008.
[11]夏其乐,陈健初,吴丹.杨梅叶提取物抗氧化活性的研究[J].食品科学,2004,25(8):80-83.
[12]黄海智.杨梅酚类化合物抗氧化和抗癌功能及机理研究[D].杭州:浙江大学,2015.
[13]佟岩,王淑君,周晓棉,等.杨梅素对小鼠被动皮肤过敏反应的影响[J].沈阳药科大学学报,2009,26(10):822-825.
[14]曹建平.杨梅黄酮对人肝癌细胞Hep G2自噬的诱导作用及其机制研究[D].杭州:浙江大学,2015.
[15]肖薪龙,张晨,海燕,等.利用两种溶剂浸提探究苦荞各部分化感效应[J].西北农业学报,2011,20(1):153-158.
[16]何海洋,胡春芹,丁强强,等.不同p H对光皮桦种子萌发及幼苗生长的影响[J].西南林业大学学报,2013,10(5):29-33.
[17]杨田甜,杜海荣,陈刚,等.植物化感作用的研究现状及其在农业生产中的应用[J].浙江农业学报,2012,24(2):343-348.
[18]Butcko V M,Jensen R J.Evidence of tissue-specific allelopathic activity in Euthamia graminifolia and Solidago canadensis(Asteraceae)[J].American Midland Naturalist,2002,148(2):253-262.
[19]孙岩,全炳武.模拟酸雨胁迫对3种玉米幼苗的伤害及其敏感性反应[J].延边大学农学学报,2010(3):181-184.
[20]李键,洪滔,林晗,等.人工栽培雷公藤水浸提液对杉木种子发芽的化感效应[J].北华大学学报:自然科学版,2010,11(4):353-356.
[21]贾黎明,翟明普,尹伟伦,等.油松、辽东栎混交林中生化他感作用的研究[J].林业科学,1995,31(6):491-498.
[22]丁国昌,曹光球,林思祖,等.2种杉木化感物质对杉木种子萌发的化感效应[J].福建农林大学学报,2007,36(2):134-137.
[23]王晓英,冯殿齐,张元湖,等.古树对萝卜、白菜化感作用的生物测定和作用机制研究[J].山东农业大学学报:自然科学版,2013,44(3):333-339.
[24]胡远彬.茎直黄芪的化感作用及抗氧化性研究[D].杨凌:西北农林科技大学,2013.
[25]Matsuda H,Morikawa T,TAO J,et al.Bioactive constituents of Chinese natural medicines,Ⅶ,Inhibitors of degranulation in RBL-2H3 cells and absolute stereostructures of three new diaryheptanoid glycosides from the bark of Myrica ruba[J].Chemical and Pharmaceutical Bulletin,2002,50(2):208-215.
[26]夏其乐,陈健初,吴丹.杨梅叶提取物抗氧化活性的研究[J].食品科学,2004,25(8):80-83.
[27]董悦涵.杨梅树皮提取物中总黄酮快速分析方法研究[J].南方农业学报,2011,42(10):1263-1266.
[28]李国成,陈楚雄,罗嘉玲,等.杨梅叶降血糖有效部位的化学成分研究[J].中草药,2011,42(5):863-865.
[29]谢苑,高素萍.白三叶水浸液对高羊茅种子萌发及幼苗生长的影响[J].安徽农业科学,2008,36(9):3616-3618.
[30]杜吉到,屈春媛,李明,等.PEG模拟干旱胁迫对芸豆萌发的影响[J].黑龙江八一农垦大学学报,2015,26(5):1-5.
[31]Pandey D K,Kauraw L P,Bhan V M.Inhibitory effect of parthenium(Parthenium hysterophorus L.)residue on growth of water hyacinth(Eichhornia crassipes mart solms).Ⅰ.Effect of leaf residue[J].Journal of Chemical Ecology,1993,19:2651-2662.