干粉性抗凝剂测定血沉的初步观察
|
摘要
目的初步观察用干粉抗凝剂测定血沉的效果。方法选取两根内置109mmol/L枸橼酸钠0.4 ml的血沉测定管,分别作为标准管和干粉管(80℃烘干后),随机选取31例病人,抽取新鲜血液4.0 ml,分别按随机顺序注入标准管和干粉管,新鲜血量分别为1.6 ml及2.0 ml,混匀后以同样仪器测定血沉值。结果标准管、干粉管血沉值分别为(25.03±24.38)mm/hr,(38.15±33.44)mm/hr,两者比较差异有统计学意义(t=0.041,P<0.05);散点图示干粉管血沉值(Y)与标准管血沉值(X)呈线性正相关,经回归分析两者关系的回归方程为Y=1.31X-5.46(r=0.91,F=279.72,P<0.01)。结论目前使用的标准血沉测定由于血液受到抗凝剂的稀释,与使用干粉性同样抗凝剂相比,其血沉值有一定降低。使用干粉性抗凝剂测定血沉更能真实反映临床实际测定值。
Objective To observe preliminarily the effect of measuring the ESR with dry powder anticoagulant. Methods Two blood sedimentation tubes with 0. 4 ml 109 mmol/L sodium citrate were used as standard tubr and dry powder tube( after drying at80 ℃). 31 patients were selected randomly,and 4. 0 ml of fresh blood was drawn in random order. The fresh blood were injected into the standard tube and the dry powder tube. The volume were 1. 6 ml and 2. 0 ml respectively. After mixing,the blood sedimentation rate was measured with the same instrument. Results The ESR of the standard tube and dry powder tube were( 25. 03± 24. 38) mm/hr and( 38. 15 ± 33. 44) mm/hr respectively; the difference is statistically significant( t = 0. 041,P < 0. 05). The scatterplot shows that the there was a linear positive correlation between the ESR of dry powder tube( Y) and the standard tube( X),and the regression equation for the relationship was Y = 1. 31 X-5. 46( r = 0. 91,F = 279. 72,P < 0. 01). Conclusion Compared with the use of the same anticoagulant,the current standard ESR measuring is certainly reduced because it is diluted by anticoagulant. The use of dry powder anticoagulant to determine ESR can reflect the actual clinical value more effectively.
Objective To observe preliminarily the effect of measuring the ESR with dry powder anticoagulant. Methods Two blood sedimentation tubes with 0. 4 ml 109 mmol/L sodium citrate were used as standard tubr and dry powder tube( after drying at80 ℃). 31 patients were selected randomly,and 4. 0 ml of fresh blood was drawn in random order. The fresh blood were injected into the standard tube and the dry powder tube. The volume were 1. 6 ml and 2. 0 ml respectively. After mixing,the blood sedimentation rate was measured with the same instrument. Results The ESR of the standard tube and dry powder tube were( 25. 03± 24. 38) mm/hr and( 38. 15 ± 33. 44) mm/hr respectively; the difference is statistically significant( t = 0. 041,P < 0. 05). The scatterplot shows that the there was a linear positive correlation between the ESR of dry powder tube( Y) and the standard tube( X),and the regression equation for the relationship was Y = 1. 31 X-5. 46( r = 0. 91,F = 279. 72,P < 0. 01). Conclusion Compared with the use of the same anticoagulant,the current standard ESR measuring is certainly reduced because it is diluted by anticoagulant. The use of dry powder anticoagulant to determine ESR can reflect the actual clinical value more effectively.
引文
[1]李秀芳.一次性塑料真空血沉管与传统魏氏血沉管的临床应用对照研究[J].医学动物防制,2013,29(8):942-943.
[2]郭莉,卢美萍,董桂娟,等.巨噬细胞活化综合征的临床和实验室特征分析[J].中国当代儿科杂志,2017,19(2):188-192.
[3]NISHIMOTO N,TAKAQI N.Assessment of the validity of the 28-joint disease activity score using erythrocyte sedimentation rate(DAS28-ESR)as a disease activity index of rheumatoid arthritis in the efficacy evaluation of 24-week treatment with tocilizumab:subanalysis of theSATORI study[J].Mod Rheumatol,2010,20(6):539-547.
[4]YAYAN J.Erythrocyte sedimentation rate as a marker for coronary heart disease[J].Vascular Health&Risk Management,2012,8(2):219-223.
[5]BIRD P,NICHOLLS D,BARRETT R,et al.Longitudinal study of clinical prognostic factors in patients with early rheumatoid arthritis:the PREDICT study[J].Int J Rheum Dis,2017,20(4):460-468.
[6]HAPPE MR,BATTAFARANO DF,DOOLEY DP,et al.Validation of the Diesse Mini-Ves Erythrocyte Sedimentation Rate(ESR)Analyzer Using the Westergren ESR Method in Patients With Systemic Inflammatory Conditions[J].American Journal of Clinical Pathology,2002,118(1):14-17.
[7]洪伟.国际血液学标准化委员会(ICSH)关于血沉测定的建议[J].国外医学生物化学与检验分册,1994,16(3):125-126.
[8]孙荣荣,任淑贞.血沉参数检验与标准物质的现状及问题[J].上海计量测试,2011,38(5):2-4,7.
[9]LISTED N.Guidelines on selection of laboratory tests for monitoring the acute phase response.International Committee for Standardization in Haematology(expert panel on blood rheology)[J].Journal of Clinical Pathology,1988,41(11):1203-1212.
[10]LISTED N.ICSH recommendations for measurement of erythrocyte sedimentation rate.International Council for Standardization in Haematology(Expert Panel on Blood Rheology)[J].J Clin Pathol,1993,46(3):198-203.
[11]尚红,王毓三,申子瑜.全国临床检验操作规程[M].4版.北京:人民卫生出版社,2015:25-26.
[12]PLEBANI M,PIVA E.Erythrocyte sedimentation Rate Use of Fresh Blood for Quality Control[J].Am J Clin Pathol,2002,117(4):621-626.
[13]SHELAT SG,CHACOSKY D,SHIBUTANI S.Differences in erythrocyte sedimentation rates using the Westergren method and a centrifugation method[J].American Journal of Clinical Pathology,2008,130(1):127-130.
[14]PLEBANI M,PIVA E.Erythrocyte sedimentation Rate Use of Fresh Blood for Quality Control[J].Am J Clin Pathol,2002,117(4):621-626.
[15]乔庆红,傅颖,郭春林,等.血沉、C反应蛋白、CEA检测在消化道恶性肿瘤中的意义[J].中国实用医药,2015,10(2):47-48.
[16]付丽琴.两种不同抗凝剂全血在血沉检测中的应用[J].世界最新医学信息文摘,2016,16(89):138.
[2]郭莉,卢美萍,董桂娟,等.巨噬细胞活化综合征的临床和实验室特征分析[J].中国当代儿科杂志,2017,19(2):188-192.
[3]NISHIMOTO N,TAKAQI N.Assessment of the validity of the 28-joint disease activity score using erythrocyte sedimentation rate(DAS28-ESR)as a disease activity index of rheumatoid arthritis in the efficacy evaluation of 24-week treatment with tocilizumab:subanalysis of theSATORI study[J].Mod Rheumatol,2010,20(6):539-547.
[4]YAYAN J.Erythrocyte sedimentation rate as a marker for coronary heart disease[J].Vascular Health&Risk Management,2012,8(2):219-223.
[5]BIRD P,NICHOLLS D,BARRETT R,et al.Longitudinal study of clinical prognostic factors in patients with early rheumatoid arthritis:the PREDICT study[J].Int J Rheum Dis,2017,20(4):460-468.
[6]HAPPE MR,BATTAFARANO DF,DOOLEY DP,et al.Validation of the Diesse Mini-Ves Erythrocyte Sedimentation Rate(ESR)Analyzer Using the Westergren ESR Method in Patients With Systemic Inflammatory Conditions[J].American Journal of Clinical Pathology,2002,118(1):14-17.
[7]洪伟.国际血液学标准化委员会(ICSH)关于血沉测定的建议[J].国外医学生物化学与检验分册,1994,16(3):125-126.
[8]孙荣荣,任淑贞.血沉参数检验与标准物质的现状及问题[J].上海计量测试,2011,38(5):2-4,7.
[9]LISTED N.Guidelines on selection of laboratory tests for monitoring the acute phase response.International Committee for Standardization in Haematology(expert panel on blood rheology)[J].Journal of Clinical Pathology,1988,41(11):1203-1212.
[10]LISTED N.ICSH recommendations for measurement of erythrocyte sedimentation rate.International Council for Standardization in Haematology(Expert Panel on Blood Rheology)[J].J Clin Pathol,1993,46(3):198-203.
[11]尚红,王毓三,申子瑜.全国临床检验操作规程[M].4版.北京:人民卫生出版社,2015:25-26.
[12]PLEBANI M,PIVA E.Erythrocyte sedimentation Rate Use of Fresh Blood for Quality Control[J].Am J Clin Pathol,2002,117(4):621-626.
[13]SHELAT SG,CHACOSKY D,SHIBUTANI S.Differences in erythrocyte sedimentation rates using the Westergren method and a centrifugation method[J].American Journal of Clinical Pathology,2008,130(1):127-130.
[14]PLEBANI M,PIVA E.Erythrocyte sedimentation Rate Use of Fresh Blood for Quality Control[J].Am J Clin Pathol,2002,117(4):621-626.
[15]乔庆红,傅颖,郭春林,等.血沉、C反应蛋白、CEA检测在消化道恶性肿瘤中的意义[J].中国实用医药,2015,10(2):47-48.
[16]付丽琴.两种不同抗凝剂全血在血沉检测中的应用[J].世界最新医学信息文摘,2016,16(89):138.