一株产黑色素海洋真菌的分离、鉴定及色素性质的研究
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摘要
从海州湾筛选产黑色素的真菌,确定其分类地位,并对其所产色素的性质进行研究。对海州湾海域采集的海水、海葵、海泥等样品进行富集培养,筛选具有产色素能力的真菌。利用摇瓶发酵进行复筛,筛选得到产黑色素的菌株,对筛选得到的菌株进行鉴定,然后对其所产色素进行粗提,并对性质进行测定。通过筛选获得一株产黑色素丝状真菌菌株CXPF01,结合形态学观察和ITS分子序列分析将CXPF01鉴定为Cladosporium cladosporioides。所产黑色素在220 nm处具有最大吸收值。光照对色素的稳定性影响不显著,高温和酸碱对黑色素稳定性具有显著影响。该色素对Staphylococcus aureus和Escherichia coli具有抑制作用。该真菌所产黑色素对在光照条件下有较好的稳定性并具有抑菌作用。
Our aims are to isolate melanin-producing fungi from Haizhou Bay,to clarify the taxonomic status,and to study theproperties of the melanin. The pigments-producing fungi were isolated from the collected samples of sea water,sea anemone,and sea mudfrom Haizhou Bay with the method of enrichment culture. The melanin-producing fungi were re-screened by shake flask fermentation. Then themelanin-producing fungi were identified and the properties of the crudly extracted melanin were measured. The strain CXPF01 was obtained andidentified as Cladosporium cladosporioides by its morphological and ITS sequence homology. The pigment had maximum absorption value at 220 nm. There was non-significant effect of light on the pigment while high temperature and alkalinity or acidity had a remarkable impact on it. Thepigment presented inhibitory effect on Staphylococcus aureus and Escherichia coli. Conclusively,the melanin-producing fungus CXPF01 wasobtained,from which the melanin was stable in light and it had antibacterial activity.
Our aims are to isolate melanin-producing fungi from Haizhou Bay,to clarify the taxonomic status,and to study theproperties of the melanin. The pigments-producing fungi were isolated from the collected samples of sea water,sea anemone,and sea mudfrom Haizhou Bay with the method of enrichment culture. The melanin-producing fungi were re-screened by shake flask fermentation. Then themelanin-producing fungi were identified and the properties of the crudly extracted melanin were measured. The strain CXPF01 was obtained andidentified as Cladosporium cladosporioides by its morphological and ITS sequence homology. The pigment had maximum absorption value at 220 nm. There was non-significant effect of light on the pigment while high temperature and alkalinity or acidity had a remarkable impact on it. Thepigment presented inhibitory effect on Staphylococcus aureus and Escherichia coli. Conclusively,the melanin-producing fungus CXPF01 wasobtained,from which the melanin was stable in light and it had antibacterial activity.
引文
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[13]叶明,陈晓,朱立,等.暗盘孢属YM421黑色素稳定性及其抗氧化活性[J].菌物学报,2010,29(2):254-260.
[14]戴德慧,胡伟莲.丝状真菌D447胞外黑色素性质的研究[J].安徽农业科学,2013,8(8):3303-3305.
[2]Toledo AV,Franco MEE,Yanil Lopez SM,et al.Melanins in fungi:Types,localization and putative biological roles[J].Physiologicaland Molecular Plant Pathology,2017,99:2-6.
[3]Helene C.Eisenman,Arturo Casadevall.Synthesis and assemblyof fungal melanin[J].Applied Microbiology and Biotechnology,2012,93(3):931-940.
[4]Zou Y,Hu W,Ma K,et al.Physicochemical properties andantioxidant activities of melanin and fractions from Auricularia auricula fruiting bodies[J].Food Science and Biotechnology,2015,24(1):15-21.
[5]王玉洁,符坚,刘楠,等重要的生物资源黑色素及其功能的机理[J].氨基酸和生物资源,2003,25(1):12-14.
[6]van De Sande WW,de Kat J,Coppens J,et al.Melanin biosynthesisin Madurella mycetomatis and its effect on susceptibility toitraconazole and ketoconazole[J].Microbes Infect,2007,9(9):1114-1123.
[7]倪丽娜.一株高产黑色素细菌的分离及鉴定[J].微生物学通报,2004,31(1):55-59.
[8]Cordero RJB,Casadevall A.Functions of fungal melanin beyondvirulence[J].Fungal Biology Reviews,2017,31(2):99-112.
[9]Nosanchuk JD,Arturo C.The contribution of melanin to microbialpathogenesis[J].Cellular Microbiology,2003,5(4):203-223.
[10]李鹏,苗增良,王健鑫.一株产黑褐色色素海洋放线菌的研究[J].海洋环境科学,2011,30(5):689-693.
[11]郑晨娜,方柏山,罗菊香,等.链霉菌G-HD-4产黑色素的提取及理化性质[J].华侨大学学报:自然版,2009,30(3):292-296.
[12]徐静,吴少杰,阎斌伦,等.产黑色素海洋放线菌的分离、鉴定和产色素条件优化[J].微生物学通报,2012,39(12):1711-1719.
[13]叶明,陈晓,朱立,等.暗盘孢属YM421黑色素稳定性及其抗氧化活性[J].菌物学报,2010,29(2):254-260.
[14]戴德慧,胡伟莲.丝状真菌D447胞外黑色素性质的研究[J].安徽农业科学,2013,8(8):3303-3305.