一株黑果枸杞内生真菌RML6的黄色素稳定性研究
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摘要
植物内生真菌具有产天然活性物质的潜能,亦是天然色素的来源之一,本研究从黑枸杞叶部分离筛选得到产黄色素的内生真菌RML6,经鉴定该菌为Coprinellus radians,该菌株所产黄色素在341 nm波长处有最大吸收峰。从温度、pH、光照、金属离子、氧化剂与还原剂等方面探究黑枸杞内生真菌RML6所产黄色素的稳定性。结果表明该色素在自然光下放置8 h黄色素光吸收值无显著变化,但对紫外较为敏感应尽量避光存放;在pH 6~10,20~100℃下不受太大影响;金属离子中,Na~+、K~+、Mg~(2+)、Cu~(2+)、Fe~(3+)对该色素有护色或增色作用,Zn~(2+)、Mn~(2+)、Ca~(2+)对色素有明显的影响;H_2O_2、NaHSO_3对该色素有较强的破坏力;常见几种添加剂对色素无太大影响,同时蔗糖对其还具有明显的增色效应。该色素可广泛用于多个用途。
Endophytic fungi in plants have the potential of producing natural active substances and it is one of the sources of natural pigments. In this study,endophytic fungi RML6 is screened out from the leaves of Lycium ruthenicum Murr.,and the strain is identified as Coprinellus radians,The yellow pigment which produced at this strain has the highest absorption peak at 341 nm. The stability of the yellow pigment which produced by the endophytic fungus RML6 of Lycium ruthenicum Murr was investigated from the aspects of temperature,pH,light,metal ion,oxidant and reducing agent.The results show that the pigment has no significant change in the light absorption value of yellow pigment for 8 hours under natural light,but it is less sensitive to UV storage. It is not affected much at pH 6-10 and 20-100 ℃. In the metal ions,Na+,k+,Mg~(2+),Cu~(2+),Fe~(3+) have color protection or burnish effect on the pigment,Zn~(2+),Mn~(2+),Ca~(2+) have a significant effect on the pigment. H_2O_2 and NaHSO_3 have strong destructive power to the pigment though several common additives have no effect on the pigment. Meanwhile,sucrose also has a significant hyperchromic effect on it. So the pigment can be used for a wide range of applications.
Endophytic fungi in plants have the potential of producing natural active substances and it is one of the sources of natural pigments. In this study,endophytic fungi RML6 is screened out from the leaves of Lycium ruthenicum Murr.,and the strain is identified as Coprinellus radians,The yellow pigment which produced at this strain has the highest absorption peak at 341 nm. The stability of the yellow pigment which produced by the endophytic fungus RML6 of Lycium ruthenicum Murr was investigated from the aspects of temperature,pH,light,metal ion,oxidant and reducing agent.The results show that the pigment has no significant change in the light absorption value of yellow pigment for 8 hours under natural light,but it is less sensitive to UV storage. It is not affected much at pH 6-10 and 20-100 ℃. In the metal ions,Na+,k+,Mg~(2+),Cu~(2+),Fe~(3+) have color protection or burnish effect on the pigment,Zn~(2+),Mn~(2+),Ca~(2+) have a significant effect on the pigment. H_2O_2 and NaHSO_3 have strong destructive power to the pigment though several common additives have no effect on the pigment. Meanwhile,sucrose also has a significant hyperchromic effect on it. So the pigment can be used for a wide range of applications.
引文
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2 Li H(李涵).Research on diversity and metabolite activity of plant endop Hytic Fungi[D];Journal of Jilin University.
3 Dong DH(董得红).Qaidam Lycium ruthenicum Murr[J].Chinese Turkish(中国土族),2014,(02):25-26.
4 Yang B(杨斌),Wang XD(王向未).Application and development of Lycium ruthenicum Murr and its functional components in food industry[J].Light Industry Science and Technology(轻工科技),2014,30(10):22-23.
5 Xu YJ(许友姣),Lu LX(陆利霞),Xiong XH(熊晓辉).Technologies to improing The Stability of the natural food colorants[J].Food R D(食品研究与开发),2008,(02):188-192.
6 Wang J(王君),Zhang BS(张宝善).Research progress on natural pigments produced by microorganisms[J].Microbiology(微生物学通报),2007,(3):580-583.
7 Liu W(刘维),Ruan LY(阮璐雅),Guan CL(管成林),et al.Study on stability of red pigments produced by an ednop Hytic fungus H1B1 from Dendrobium officinale[J].China Food Addit(中国食品添加剂),2016,(01):71-75.
8 Zhang LQ(张立强),Liang HB(梁华兵),Ai TS(艾桃山),et al.Characterization of a Red Pigments Producing Fungus and Study on the Stabilty of the Red Pigments[J].Hubei Agric Sci(湖北农业科学),2015,54:2863-2865.
9 Li WJ(李文娟),Lv JL(吕嘉枥),Du B(杜冰),et al.Study on Properties of Pigment Produced during the Liquid Fermentation of Eurotium cristatum[J].Food Ind(食品工业),2016,37(1):27-31.
10 Zhou J(周静),Liu Z(刘姝),Hu CY(胡晟源),et al.Isolation and identification of yellow pigment producing marine fungi strain and characterization of the melanin pigment[J].Sci Technol Food Ind(食品工业科技),2017,38:217-220.
11 Yang L(杨柳),Wang QQ(王青青),Wu JX(吴俊贤),et al.Stability of yellow pigment from Lachnum sp.[J].Microbiology China(微生物学通报),2016,43(5):1042-1050.
12 Li YT(李玉婷),Lv JL(吕嘉枥).Study on stability and antioxidant activity of the yellow pigments from Eurotium cristatum[J].Microbiology China(微生物学通报),2013,40:2030-2036.
13 Lin GS(林观盛),Wang S(王松),Xia HG(夏华刚),et al.Studies on Resources of Macro-fungi in Southwest University[J].Indian J Agr Sci(安徽农业科学),2010,38:20875-20877.
14 Gao Y(高越),Xi YB(郗砚彬).A new Guanacastane type diterpenoid compound was isolated from the gibbon.J Tradit Chin Med(国际中医中药杂志),2012,34:676-676.