花生衣化学成分的分离与结构鉴定
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摘要
[目的]对花生衣中的化学成分进行研究,为花生衣的系统研究和开发利用提供物质基础。[方法]采用正相硅胶等柱色谱及高效液相色谱法进行分离纯化,并通过理化性质与波谱分析相结合的技术鉴定化合物的结构。[结果]从花生衣50%乙醇提取物中分离鉴定12个单体成分,分别为对羟基苯甲酸(1)、原儿茶酸(2)、原儿茶酸甲酯(3)、原儿茶酸乙酯(4)、对香豆酸(5)、儿茶素(6)、表儿茶素(7)、rhodonidin A(8)、槲皮素(9)、异鼠李素-3-O-β-D-葡萄糖苷(10)、异鼠李素-3-O-芸香糖苷(11)、3′,5,7-trihydroxyisoflavone-4′-methoxy-3′-O-β-glucopyranoside(12)。[结论]其中8为首次从落花生属中分离得到,3为首次从该植物中分离得到的化合物。
[Objective] To study chemical constituents from Arachis hypogaea Linn. in order to systematic research and exploitation. [Methods] The chemical compounds were isolated through Silica gel and PHPLC column chromatorgraphies,and identified by chemical and spectral analysis(1 H,13 C NMR,1 H-1 H COSY,HMQC,HMBC,and MS). [Results] Twelve compounds were obtained and identified as p-hydroxybenzoic acid(1),protocatechuic acid(2),protocatechuic acid methyl ester(3),protocatechuic acid ethyl ester(4),p-coumaric acid(5),catechin(6),epicatechin(7),rhodonidin A(8),quercetin(9),isorhamnetin-3-O-β-D-glucoside(10),isorhamnetin-3-Orutinoside(11),3',5,7-trihydroxyisoflavone-4'-methoxy-3'-O-β-glucopyranoside(12),respectively. [Conclusion]Compound 8 was obtained from Arachis genus firstly,3 was isolated from the plant for the first time.
[Objective] To study chemical constituents from Arachis hypogaea Linn. in order to systematic research and exploitation. [Methods] The chemical compounds were isolated through Silica gel and PHPLC column chromatorgraphies,and identified by chemical and spectral analysis(1 H,13 C NMR,1 H-1 H COSY,HMQC,HMBC,and MS). [Results] Twelve compounds were obtained and identified as p-hydroxybenzoic acid(1),protocatechuic acid(2),protocatechuic acid methyl ester(3),protocatechuic acid ethyl ester(4),p-coumaric acid(5),catechin(6),epicatechin(7),rhodonidin A(8),quercetin(9),isorhamnetin-3-O-β-D-glucoside(10),isorhamnetin-3-Orutinoside(11),3',5,7-trihydroxyisoflavone-4'-methoxy-3'-O-β-glucopyranoside(12),respectively. [Conclusion]Compound 8 was obtained from Arachis genus firstly,3 was isolated from the plant for the first time.
引文
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[2]张慧文.花生红衣原花青素化学成分、衍生物和生物活性研究[D].呼河浩特:内蒙古大学,2014.
[3]李孔燕.花生衣对环磷酰胺诱导的小鼠血小板减少症模型的作用研究[D].广州:暨南大学,2010.
[4]晁利平,阮静雅,刘艳霞,等.绵萆薢化学成分的分离与结构鉴定[J].天津中医药大学学报,2016,35(5):336-339.
[5]瞿璐,刘艳霞,李建良,等.祁菊叶化学成分的分离与结构鉴定[J].天津中医药大学学报,2017,36(1):57-60.
[6]董勇喆,史文中,杨圣财,等.沙葱化学成分的分离与结构鉴定II[J].天津中医药大学学报,2016,33(6):404-408.
[7]李晓霞,瞿璐,刘丽丽,等.国槐化学成分与药理作用的研究进展[J].天津中医药大学学报,2016,35(3):211-216.
[8]张祎,晁利平,陈秋,等.维药蜀葵花黄酮类成分的分离与结构鉴定[J].天津中医药大学学报,2016,35(1):36-39.
[9]李艳茸,李春,王智民,等.藏药甘青乌头化学成分研究(Ⅲ)[J].中国中药杂志,2014,39(7):1163-1167.
[10]傅志勤,黄泽豪,林婧,等.蛇附子化学成分及抗氧化活性研究[J].中草药,2015,46(11):1583-1588.
[11]朱仝飞,陈日荣,孙庆文,等.西藏胡黄连化学成分研究[J].中草药,2017,48(2):263-265.
[12]张航旗,周戚,付艳辉,等.头序瓜馥木化学成分研究[J].天然产物研究与开发,2017,29(2):245-248+244.
[13]谢雪,张宏达,温建辉,等.桂枝茯苓胶囊化学成分研究(VI)[J].中草药,2016,47(21):3795-3797.
[14]马强,房鑫,李俊,等.羊踯躅的化学成分研究[J].中草药,2018,49(5):1013-1018.
[15]张翠,刘占云,於洪建,等.黑豆种皮的酚酸类成分研究[J].中草药,2013,44(24):3440-3443.
[16] Wang CM,Hsu YM,Jhan YL,et al. Structure elucidation of procyanidins isolated from Rhododendron formosanum and their anti-oxidative and anti-bacterial activities[J].Molecules,2015,20(7):12787-12803.
[17]李蓉,杨春涛,田凯,等.东方草莓的化学成分研究[J].云南民族大学学报(自然科学版),2014,23(5):313-316.
[18]昶国平,李广雷,刘博譞,等.小花棘豆的化学成分研究[J].中医药学报,2014,42(4):15-16.
[19]张成刚,卢叶,王峥涛,等.阔刺兔唇花全草的化学成分研究[J].中草药,2014,45(22):3224-3229.
[20] Lou H,Yuan H,Yamazaki Y,et al. Alkaloids and flavonoids from peanut skins[J]. Planta medica,2001,67(4):345-349.