摘要
目的建立双波长HPLC法同时测定止痒地霜中地塞米松磷酸钠和羟苯乙酯的含量。方法采用RP-HPLC法,以Wondasil C_(18)(4.6 mm×250 mm,5μm)为色谱柱,以三乙胺溶液-甲醇-乙腈(52:43:5)为流动相;检测波长为242 nm,流速为1.0 ml/min。结果羟苯乙酯和地塞米松磷酸酯分别在21.42~64.26μg/ml(r=1.0000)和20.84~62.53μg/ml(r=0.9999)范围内有良好的线性关系,平均回收率分别为99.1%(RSD=0.41%)和99.0%(RSD=0.72%)。结论该法简便、准确、重复性好,可用于止痒地霜的质量控制。
Objective To establish a double wavelength HPLC method for determination of dexamethasone sodium phosphate and ethylparaben in Zhiyang dishuang cream.Methods The HPLC method was adopted with the Wondasil C_(18)(4.6 mm×250 mm,5 μm) and triethylamine solution-methanol-acetonitrile(52∶43∶5) as the mobile phase at the rate of 1 ml/min.The detection wavelength was 242 nm.Results The linear ranges of ethylparaben and dexamethasone phosphate were 21.42-64.26 μg/ml(r=1.0000)and 20.84-62.53μg/ml(r=0.9999)respectively.The average recoveries of the three contents were 99.1% and 99.0%,while RSD were 0.41% and 0.72%.Conclusion The method is simple,accurate,reproducible and suitable for quality control of Zhiyang dishuang cream.
引文
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