5种酢浆草属植物对低温胁迫的生理响应及抗寒性评价
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摘要
以5种酢浆草属植物为试验材料,通过人工低温和自然低温处理,观测形态特征,测定相对电导率、丙二醛(MDA)质量摩尔浓度、超氧化物歧化酶(SOD)活性、过氧化物酶(POD)活性、可溶性蛋白质量分数、游离脯氨酸质量分数和叶绿素质量分数等指标,评价其抗寒性。结果表明:在人工低温处理下,随着处理温度的降低,5种植物的生理生化指标变化趋势基本一致,相对电导率呈上升趋势;MDA质量摩尔浓度呈先下降后上升再下降趋势;SOD活性、POD活性、可溶性蛋白质量分数和游离脯氨酸质量分数呈先上升后下降趋势;叶绿素质量分数呈下降或先上升后下降趋势。通过隶属函数法综合评价5种植物的抗寒性可知,在冬季的抗寒性由大到小的顺序为黄花酢浆草、红花酢浆草、大花酢浆草、芙蓉酢浆草、紫叶酢浆草;在春季的抗寒性由大到小的顺序为红花酢浆草、黄花酢浆草、大花酢浆草、紫叶酢浆草、芙蓉酢浆草,这与自然低温处理下观测的结果一致。研究旨在从中筛选出优异抗寒的种质材料,为酢浆草属植物抗寒性研究提供种质材料和理论依据。
In order to evaluate the cold resistance of five kinds of Oxalis,we observed the changes of the indexes including morphological characteristics,relative conductivity,the level of malondialdehyde(MDA),soluble protein,free proline and chlorophyll and the activity of superoxide(SOD)and peroxidase(POD)after the treatments of artificial and natural low temperature.With the artificial temperature decreasing,the changes of physiological and biochemical indexes of five plants were somewhat similar.The activity of SOD and POD,the contents of soluble protein and free proline went up firstly,and then went down,while the relative conductivity was increased and the MDA content had a fall before a rise,and then went down.The content of chlorophyll was decreased or increased firstly and then decreased.Through evaluating the cold resistance of the five kinds of plants by the membership function method,the descending orders of cold resistance in winter and spring,separately,were O.pes-caprae,O.corymbosa,O.bowiei,O.purpurea,O.violacea,and O.corymbosa,O.pes-caprae,O.bowiei,O.violacea,and O.purpurea,which agreed with the results under natural low temperature conditions.
In order to evaluate the cold resistance of five kinds of Oxalis,we observed the changes of the indexes including morphological characteristics,relative conductivity,the level of malondialdehyde(MDA),soluble protein,free proline and chlorophyll and the activity of superoxide(SOD)and peroxidase(POD)after the treatments of artificial and natural low temperature.With the artificial temperature decreasing,the changes of physiological and biochemical indexes of five plants were somewhat similar.The activity of SOD and POD,the contents of soluble protein and free proline went up firstly,and then went down,while the relative conductivity was increased and the MDA content had a fall before a rise,and then went down.The content of chlorophyll was decreased or increased firstly and then decreased.Through evaluating the cold resistance of the five kinds of plants by the membership function method,the descending orders of cold resistance in winter and spring,separately,were O.pes-caprae,O.corymbosa,O.bowiei,O.purpurea,O.violacea,and O.corymbosa,O.pes-caprae,O.bowiei,O.violacea,and O.purpurea,which agreed with the results under natural low temperature conditions.
引文
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[15]田景花,王红霞,高仪,等.核桃属4树种展叶期抗寒性鉴定[J].园艺学报,2012,39(1):2439-2446.
[16]林兴生,林占熺,林冬梅,等.低温胁迫5种菌草的抗寒性评价[J].草业学报,2013,22(2):227-234.
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[19]张远兵,刘爱荣,方蓉.外源一氧化氮对镉胁迫下黑麦草生长和抗氧化酶活性的影响[J].草业学报,2008,17(4):57-64.
[20]王罗霞,赵志光,王锁民.一氧化氮对水分胁迫下小麦叶片活性氧代谢及膜脂过氧化的影响[J].草业学报,2006,15(4):104-108.
[21]雷帅,蒋倩,汉梅兰,等.五种花灌木对低温胁迫的生理响应及抗寒性评价[J].甘肃农业大学学报,2015,50(5):35-41.
[22]MONROY A F,SANGWAN V,DHINDSA R S.Low temperature signal transduction during cold acclimation:protein phosphatase2A as an early target for cold-inactivation[J].The Plant Journal,1998,13(5):653-660.
[23]FRY J D,LANG N S,CLIFTON R G P,et al.Freezing tolerance and carbohydrate content of low-temperature-acclimated and nonacclimated centipedgrass[J].Crop Science,1993,33(5):1051-1055.
[24]时振振,李胜,杨柯,等.盐胁迫下豌豆幼苗对内外源NO的生理生化响应[J].草业学报,2014,23(5):193-200.
[25]贺磊,游凯,李远芳,等.5种典型热带引种园林植物幼苗抗寒性研究[J].中南林业科技大学学报,2011,31(2):65-71.
[26]PRASAD T K.Mechanisms of chilling-induced oxidative stress injury and tolerance in developing maize seedlings:changes in antioxidant system,oxidation of proteins and lipids,and protease activities[J].Plant Journal,1996,10(6):1017-1026.
[27]RUBIO M C,JAMES E K,CLEMENTE M R,et al.Localization of superoxide dismutases and hydrogen peroxide in legume root nodules[J].Molecular Plant-Microbe Interactions,2004,17(12):1294-1305.
[28]MILLER A.Superoxide dismutases:active sites that save,but a protein that kills[J].Science,2004,8(2):162-168.
[29]田景花,王红霞,张志华,等.低温逆境对不同核桃品种抗氧化系统及超微结构的影响[J].应用生态学报,2015,26(5):1320-1326.
[30]武雁军,刘建辉.低温胁迫对厚皮甜瓜幼苗抗寒性生理生化指标的影响[J].西北农林科技大学学报(自然科学版),2007,35(3):139-143.
[31]任俊杰,赵爽,苏彦苹,等.春季低温胁迫对核桃抗氧化酶指标的影响[J].西北农林科技大学学报(自然科学版),2016,44(3):75-81.
[32]杜永吉,于磊,孙吉雄,等.结缕草3个品种抗寒性的综合评价[J].草业学报,2008,17(3):6-16.
[33]许桂芳,张朝阳,向佐湘.利用隶属函数法对4种珍珠菜属植物的抗寒性综合评价[J].西北林学院学报,2009,24(3):24-26.
[2]TOSTO D,HOPP E.Characterization of the nuclear ribosomal DNA unit in Oxalis tuberosa(Oxalidacea)and related species[J].Electronic Journal of Biotechnology,2008,11(3):1-17.
[3]COSTA J,FERRERO V,LOUREIRO J,et al.Sexual reproduction of the pentaploid,short-styled Oxalis pes-caprae allows the production of viable offspring[J].Plant Biology,2014,16(1):208-214.
[4]PISSARD A,ARBIZU C,GHISLAIN M,et al.Congruence between morphological and molecular markers inferred from the analysis of the intra-morphotype genetic diversity and the spatial structure of Oxalis tuberosa Mol.[J].Genetica,2008,132(1):71-85.
[5]MALICE M,MARTIN N,PISSARD A,et al.A preliminary study of the genetic diversity of Bolivian oca(Oxalis tuberosa Mol.)varieties maintained in situ and ex situ through the utilization of ISSR molecular markers[J].Genetic Resources and Crop Evolution,2007,54(4):685-690.
[6]胡甦,王永清,陶炼.三角紫叶酢浆草ISSR反应体系的建立与优化[J].草业学报,2011,20(5):142-150.
[7]陈明林,王友保.水分胁迫下外来种铜锤草和本地种酢浆草的生理指标比较研究[J].草业学报,2008,17(6):52-59.
[8]陈明林,张云华,严密,等.盐胁迫下外来种铜锤草和本地种酢浆草的生理指标比较研究[J].上海交通大学学报(农业科学版),2007,25(3):282-288.
[9]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2003:37-281.
[10]朱根海,刘祖祺,朱培仁.应用Logistic方程确定植物组织低温半致死温度的研究[J].南京农业大学学报,1986(3):11-15.
[11]张文娥,王飞,潘学军.应用隶属函数法综合评价葡萄种间抗寒性[J].果树学报,2007,24(6):849-853.
[12]LYONS J M.Chilling injury in plants[J].Annual Review of Plant Physiology,1973,24(1):445-466.
[13]MITTLER R.Oxidative stress,antioxidants and stress tolerance[J].Trends in Plant Science,2002,7(9):405-410.
[14]张家洋,郭晖,张莉,等.不同低温胁迫对南京10种道路绿化树木形态及电导率的影响[J].东北林业大学学报,2012,40(5):25-28.
[15]田景花,王红霞,高仪,等.核桃属4树种展叶期抗寒性鉴定[J].园艺学报,2012,39(1):2439-2446.
[16]林兴生,林占熺,林冬梅,等.低温胁迫5种菌草的抗寒性评价[J].草业学报,2013,22(2):227-234.
[17]VELIKOVA V,YORDANOV I,EDREVA A.Oxidative stress and some antioxidant systems in acid rain-treated bean plants:protective role of exogenous polyamines[J].Plant Science,2000,151(1):59-66.
[18]杜有新,何春林,张乐华,等.庐山若干常绿树种对冬季低温的生理生化响应[J].生态环境学报,2014,23(6):945-949.
[19]张远兵,刘爱荣,方蓉.外源一氧化氮对镉胁迫下黑麦草生长和抗氧化酶活性的影响[J].草业学报,2008,17(4):57-64.
[20]王罗霞,赵志光,王锁民.一氧化氮对水分胁迫下小麦叶片活性氧代谢及膜脂过氧化的影响[J].草业学报,2006,15(4):104-108.
[21]雷帅,蒋倩,汉梅兰,等.五种花灌木对低温胁迫的生理响应及抗寒性评价[J].甘肃农业大学学报,2015,50(5):35-41.
[22]MONROY A F,SANGWAN V,DHINDSA R S.Low temperature signal transduction during cold acclimation:protein phosphatase2A as an early target for cold-inactivation[J].The Plant Journal,1998,13(5):653-660.
[23]FRY J D,LANG N S,CLIFTON R G P,et al.Freezing tolerance and carbohydrate content of low-temperature-acclimated and nonacclimated centipedgrass[J].Crop Science,1993,33(5):1051-1055.
[24]时振振,李胜,杨柯,等.盐胁迫下豌豆幼苗对内外源NO的生理生化响应[J].草业学报,2014,23(5):193-200.
[25]贺磊,游凯,李远芳,等.5种典型热带引种园林植物幼苗抗寒性研究[J].中南林业科技大学学报,2011,31(2):65-71.
[26]PRASAD T K.Mechanisms of chilling-induced oxidative stress injury and tolerance in developing maize seedlings:changes in antioxidant system,oxidation of proteins and lipids,and protease activities[J].Plant Journal,1996,10(6):1017-1026.
[27]RUBIO M C,JAMES E K,CLEMENTE M R,et al.Localization of superoxide dismutases and hydrogen peroxide in legume root nodules[J].Molecular Plant-Microbe Interactions,2004,17(12):1294-1305.
[28]MILLER A.Superoxide dismutases:active sites that save,but a protein that kills[J].Science,2004,8(2):162-168.
[29]田景花,王红霞,张志华,等.低温逆境对不同核桃品种抗氧化系统及超微结构的影响[J].应用生态学报,2015,26(5):1320-1326.
[30]武雁军,刘建辉.低温胁迫对厚皮甜瓜幼苗抗寒性生理生化指标的影响[J].西北农林科技大学学报(自然科学版),2007,35(3):139-143.
[31]任俊杰,赵爽,苏彦苹,等.春季低温胁迫对核桃抗氧化酶指标的影响[J].西北农林科技大学学报(自然科学版),2016,44(3):75-81.
[32]杜永吉,于磊,孙吉雄,等.结缕草3个品种抗寒性的综合评价[J].草业学报,2008,17(3):6-16.
[33]许桂芳,张朝阳,向佐湘.利用隶属函数法对4种珍珠菜属植物的抗寒性综合评价[J].西北林学院学报,2009,24(3):24-26.