高效液相色谱-质谱联用法测定北沙参中欧前胡素和异欧前胡素的含量
|
摘要
目的建立北沙参中欧前胡素和异欧前胡素含量测定的方法。方法采用高效液相色谱-质谱法:色谱柱:赛默飞世尔Accucore C18(100 mm×2.1 mm,2.6μm);流动相:5 mmol·L-1甲酸溶液-甲醇(80∶20,v/v);流速:0.2 m L·min-1,柱温:20℃。结果欧前胡素和异欧前胡素均在10~5000ng·m L-1与峰面积具有良好的线性关系,平均加样回收率分别为99.3%和99.1%,RSD分别为1.3%和1.1%(n=6)。结论本方法能够快速、准确、方便的检测北沙参中欧前胡素和异欧前胡素的含量,可作为北沙参质量评价的依据。
Objective To establish a liquid chromatography/tandem mass spectrometry(LC-MS/MS) method to determinate imperatorin and isoimperatorin in Glehniae Radix. Methods LC-MS/MS method was used. The column was Thermo Fisher Accucore C18(100 mm×2.1 mm, 2.6 μm). The mobile phase was 5 mmol·L-1 formic acid solution-methanol(80∶20, v/v), the flow rate was 0.2 m L·min-1, and the temperature was 20 ℃. Results The linear range of both imperatorin and isoimperatorin was 10-5000 ng·m L-1. The average recovery was 99.3% and 99.1%, with RSD of 1.3% and 1.1%(n = 6). Conclusion The method can be used to determine the content of imperatorin and isoimperatorin in Glehniae Radix rapidly, accurately and conveniently, and to control the quality of Glehniae Radix.
Objective To establish a liquid chromatography/tandem mass spectrometry(LC-MS/MS) method to determinate imperatorin and isoimperatorin in Glehniae Radix. Methods LC-MS/MS method was used. The column was Thermo Fisher Accucore C18(100 mm×2.1 mm, 2.6 μm). The mobile phase was 5 mmol·L-1 formic acid solution-methanol(80∶20, v/v), the flow rate was 0.2 m L·min-1, and the temperature was 20 ℃. Results The linear range of both imperatorin and isoimperatorin was 10-5000 ng·m L-1. The average recovery was 99.3% and 99.1%, with RSD of 1.3% and 1.1%(n = 6). Conclusion The method can be used to determine the content of imperatorin and isoimperatorin in Glehniae Radix rapidly, accurately and conveniently, and to control the quality of Glehniae Radix.
引文
[1]中国药典2015年版.一部[S].2015:100.
[2]苏星,李相坤,吴弢,等.北沙参药材薄层色谱指纹图谱研究[J].中药材,2012,35(2):210-213.
[3]郑旭光,陈钟,项峰,等.河北道地药材北沙参HPLCPDA指纹图谱研究[J].药物分析杂志,2013,31(9):1683-1688.
[4]王平,吴赵云,吴树华,等.不同产地北沙参中欧前胡素和异欧前胡素含量分析[J].上海中医药杂志,2011,45(2):72-74.
[5]牛韬.HPLC法测定硫磺熏蒸与未熏蒸北沙参中5种香豆素类成分的含量[J].中国药房,2015,26(27):3836-3838.
[6]刘伟,李中燕,田艳,等.北沙参的化学成分及药理作用研究进展[J].国际药学研究杂志,2013,40(3):291-294.
[7]李建业,刘运周,张薇,等.北沙参对小鼠免疫功能的影响研究[J].中国实验诊断学,2012,16(9):1599-1601.
[8]周燕萍.沙参麦冬汤对大鼠放射性肺炎的防治作用及机制研究[D].武汉:湖北中医药大学,2011.
[9]李建刚,李庆典.沙参多糖对自由基的清除作用[J].中国酿造,2011,(3):66-68.
[10]Kenney DM,Geschwindt RD,Kary MR,et al.Detection of newly diagnosed bladder cancer,bladder cancer recurrence and bladder cancer in patients hematuria using quantitative RT-PCR of urinary surviving[J].Tumour Biol,2007,28(2):57-62.
[2]苏星,李相坤,吴弢,等.北沙参药材薄层色谱指纹图谱研究[J].中药材,2012,35(2):210-213.
[3]郑旭光,陈钟,项峰,等.河北道地药材北沙参HPLCPDA指纹图谱研究[J].药物分析杂志,2013,31(9):1683-1688.
[4]王平,吴赵云,吴树华,等.不同产地北沙参中欧前胡素和异欧前胡素含量分析[J].上海中医药杂志,2011,45(2):72-74.
[5]牛韬.HPLC法测定硫磺熏蒸与未熏蒸北沙参中5种香豆素类成分的含量[J].中国药房,2015,26(27):3836-3838.
[6]刘伟,李中燕,田艳,等.北沙参的化学成分及药理作用研究进展[J].国际药学研究杂志,2013,40(3):291-294.
[7]李建业,刘运周,张薇,等.北沙参对小鼠免疫功能的影响研究[J].中国实验诊断学,2012,16(9):1599-1601.
[8]周燕萍.沙参麦冬汤对大鼠放射性肺炎的防治作用及机制研究[D].武汉:湖北中医药大学,2011.
[9]李建刚,李庆典.沙参多糖对自由基的清除作用[J].中国酿造,2011,(3):66-68.
[10]Kenney DM,Geschwindt RD,Kary MR,et al.Detection of newly diagnosed bladder cancer,bladder cancer recurrence and bladder cancer in patients hematuria using quantitative RT-PCR of urinary surviving[J].Tumour Biol,2007,28(2):57-62.