HPLC法测定防感香佩包中欧前胡素和异欧前胡素的含量
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摘要
目的建立高效液相色谱(HPLC)法同时测定防感香佩包中欧前胡素及异欧前胡素的含量。方法采用Agilent ZORBAX SB-C_(18)(150 mm×4.6 mm,5μm)色谱柱,流动相为甲醇-水(62∶38),等度洗脱,柱温:25℃,流速:1.0 mL·min~(-1),检测波长:254 nm,进样量:20μL。结果欧前胡素在1.056～31.68 mg·L~(-1)范围内呈良好的线性关系(r=0.999 7),平均加样回收率为100.01%(n=9);异欧前胡素在1.118～33.54 mg·L~(-1)范围内呈良好的线性关系(r=0.999 8),平均加样回收率为100.20%(n=9)。结论该方法简单、准确、稳定,适用于防感香佩包的质量控制。
Objective To establish an HPLC method for determination of imperatorin and isoimperatorin in Antiinfluenza Sachets. Methods The samples were analyzed on a Agilent ZORBAX SB-C_(18)(150 mm×4.6 mm,5 μm)with the mobile phase of methanol-water wavelength was set at 254 nm,and the injection volume was 20 μL. Results The linear ranges of imperatorin and isoimperatorin were 1.056-31.68 mg·L~(-1)(r=0.999 8)and 1.118-33.54 mg·L~(-1)(r=0.999 7),respectively. The average recovery was 100.01% for imperatorin(n=9), 100.20% for isoimperatorin(n=9). Conclusion This method is simple,sensitive,and accurate,which could be applicable to the quality control of Anti-influenza Sachets.
Objective To establish an HPLC method for determination of imperatorin and isoimperatorin in Antiinfluenza Sachets. Methods The samples were analyzed on a Agilent ZORBAX SB-C_(18)(150 mm×4.6 mm,5 μm)with the mobile phase of methanol-water wavelength was set at 254 nm,and the injection volume was 20 μL. Results The linear ranges of imperatorin and isoimperatorin were 1.056-31.68 mg·L~(-1)(r=0.999 8)and 1.118-33.54 mg·L~(-1)(r=0.999 7),respectively. The average recovery was 100.01% for imperatorin(n=9), 100.20% for isoimperatorin(n=9). Conclusion This method is simple,sensitive,and accurate,which could be applicable to the quality control of Anti-influenza Sachets.
引文
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[11]舒翔,叶小春,陈军,等.高效液相色谱法同时测定白芷中欧前胡素和异欧前胡素的含量[J].中国医院药学杂志,2017,37(5):427-429.
[2]纪战尚,徐建涛,徐涛,等.防感香袋防治脾虚小儿反复呼吸道感染58例[J].中国中西医结合消化杂志,2010,18(1):51-52.
[3]刘龙,岳小强,王丽娜,等.辟秽防感香囊预防流行性感冒的疗效及其免疫调节机制[J].中西医结合学报,2010,8(10):949-954.
[4]张倩,陈进春.白芷活性成分的研究进展[J].中医临床研究,2016,8(28):145-146.
[5]刘成全,韩新民.参芪固本汤加防感香囊治疗儿童反复呼吸道感染68例临床观察[J].中国中医急症,2013,22(6):900-901.
[6]国家药典委员会.中华人民共和国药典(一部)[M].北京:中国医药科技出版社,2015:105.
[7]CHEN X,SUN W,GIANARIS N G,et al.Furanocoumarins are a novel class of modulators for the transient receptor potential vanilloid type 1(TRPV1)channel[J].Journal of Biological Chemistry,2014,289(14):9600-9610.
[8]TSASSI V B,HUSSAIN H,MEFFO B Y,et al.Antimicrobial coumarins from the stem bark of Afraegle paniculata[J].Natural Product Communications,2010,5(4):559-561.
[9]伍冠一,陈佳妤,徐小丽,等.欧前胡素的中药源与药理作用研究进展[J].中医药导报,2017,23(20):54-56.
[10]王梦月,贾敏如,马逾英,等.白芷中四种线型呋喃香豆素类成分药理作用研究[J].天然产物研究与开发,2010,22(3):485-489.
[11]舒翔,叶小春,陈军,等.高效液相色谱法同时测定白芷中欧前胡素和异欧前胡素的含量[J].中国医院药学杂志,2017,37(5):427-429.