龙葵粗多糖对小鼠体内免疫系统的影响
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摘要
[目的]探讨龙葵粗多糖对小鼠体内免疫系统的影响.[方法]取雄性BALB/C小鼠随机分为空白对照组和龙葵粗多糖高、中、低(800,400,200 mg/kg)剂量组,空白对照组灌胃给予生理盐水,龙葵粗多糖高、中、低剂量组灌胃给予相应剂量的龙葵粗多糖,每日1次,连续28 d.检测小鼠胸腺脾脏指数.取小鼠脾脏细胞,分别与脂多糖、刀豆蛋白共同培养,利用CCK-8检测脾淋巴细胞数量变化情况及小鼠B,T淋巴细胞转化情况.采用LDH法检测小鼠脾细胞与YAC-1细胞联合孵育时的NK细胞活性.[结果]龙葵粗多糖高、中、低剂量组胸腺指数及脾脏指数,B,T淋巴细胞增殖转化、NK细胞活性均明显高于空白对照组(P<0.05,P<0.01).[结论]龙葵粗多糖可提高小鼠体内B,T细胞及NK细胞的活性,从而提高体内非特异性免疫与特异性免疫功能.
OBJECTIVE To explore the effects of crude polysaccharide from Solanum nigrum L. on immune system of mice. METHODS Male BALB/C mice were randomly divided into control group and high, medium and low dose of Solanum nigrum L.polysaccharide groups(800,400,200 mg/kg). The mice in the control group were given normal saline by gavage, while the mice in the high, medium and low dose groups were given corresponding dose of Solanum nigrum L.polysaccharide, once a day for 28 days. The thymus and spleen index of mice were measured. Mouse spleen cells were co-cultured with LPS and ConA, respectively. CCK 8 was used to detect the number of splenic lymphocytes and the transformation of B and T lymphocytes in mice. LDH assay was used to detect the activity of NK cells when mouse spleen cells were incubated with YAC-1 cells. RESULTS The thymus index, spleen index, B and T lymphocyte transformation and NK cell activity in the high, medium and low dose group were significantly higher than those in the control group(P<0.05, P<0.01). CONCLUSION Solanum nigrum L. polysaccharide could enhance the activity of B and T lymphocytes and NK cells in mice, so as to improve the function of nonspecific and specific immunity in vivo.
OBJECTIVE To explore the effects of crude polysaccharide from Solanum nigrum L. on immune system of mice. METHODS Male BALB/C mice were randomly divided into control group and high, medium and low dose of Solanum nigrum L.polysaccharide groups(800,400,200 mg/kg). The mice in the control group were given normal saline by gavage, while the mice in the high, medium and low dose groups were given corresponding dose of Solanum nigrum L.polysaccharide, once a day for 28 days. The thymus and spleen index of mice were measured. Mouse spleen cells were co-cultured with LPS and ConA, respectively. CCK 8 was used to detect the number of splenic lymphocytes and the transformation of B and T lymphocytes in mice. LDH assay was used to detect the activity of NK cells when mouse spleen cells were incubated with YAC-1 cells. RESULTS The thymus index, spleen index, B and T lymphocyte transformation and NK cell activity in the high, medium and low dose group were significantly higher than those in the control group(P<0.05, P<0.01). CONCLUSION Solanum nigrum L. polysaccharide could enhance the activity of B and T lymphocytes and NK cells in mice, so as to improve the function of nonspecific and specific immunity in vivo.
引文
[1] 刘利国,郭喜宝,姜小晶.龙葵的研究进展[J].中医药学刊,2006,24(7):1357-1358.
[2] 梅全喜.广东地产药材研究[M].广州:广东科技出版社,2011:247-250.
[3] 周新兰,何祥久,周光雄,等.龙葵全草皂苷类化学成分研究[J].中草药,2006,37(11):1618-1621.
[4] 赵莹,刘飞,娄红祥.龙葵化学成分研究[J].中药材,2010,33(4):555-556.
[5] Sikdar M,Dutta U.Traditional phytotherapy among the nath people of assam[J].Ethno-Med,2008,2(1):39-45.
[6] Jain R,Sharma A,Gupta S,et al..Solanum nigrum:current perspectives on therapeutic properties[J].Altern Med Rev,2011,16(1):78-85.
[7] 尚庆辉,解玉怀,张桂国,等.植物多糖的免疫调节作用及其机制研究进展[J].动物营养学报,2015,27(1):49-58.
[8] 章世元,徐春燕,董晓芳,等.苜蓿多糖和黄芪多糖对肉仔鸡淋巴细胞增殖的影响[J].动物营养学报,2010,22(3):670-674.
[9] Kim HS,Kim YJ,Lee HK,et al..Activation of macrophages by polysaccharide isolated from Paecilomyces cicadae through toll-like receptor 4[J].Food Chem Toxicol,2012,50(9):3190-3197.
[10] 王淑萍,郭艳平.龙葵多糖提取分离工艺研究[J].信阳师范学院学报:自然科学版,2011,24(2):258-260.
[11] 张淑杰,康玉凡.天然活性多糖研究进展[J].食品工业科技,2017,38(2):378-382,389.
[12] 刘鹭,潘道东,曾小群,等.硒化乳酸菌胞外多糖对体外培养小鼠脾淋巴细胞分泌NO、IL-4及IL-12的影响[J].中国食品学报,2014,14(3):17-21.
[13] 赵磊,王成涛,籍保平.鹿茸水提取物及其蛋白酶解物对小鼠脾淋巴细胞增殖的影响[J].北京工商大学学报:自然科学版,2011,29(3):20-27.
[14] 秦乐,陈新梅,邢佰颖,等.昆仑雪菊总黄酮对小鼠胸腺指数及脾脏指数的影响[J].山东化工,2017,46(15):41-42.
[15] Liu LL,Pfefferle A,Yi SV,et al..Harnessing adaptive natural killer cells in cancer immunotherapy[J].Mol Oncol,2015,9(10):1904-1917.
[16] 张辉,李玉虎.中药复方促使T淋巴细胞增殖的实验研究[J].中国实用医药,2009,4(1):1-2.
[17] Kuang P,Deng H,Cui H,et al..Suppressive effects of sodium fluoride on cultured splenic lymphocyte proliferation in mice[J].Oncotarget,2016,7(38):61905-61915.
[18] 余强.黑灵芝多糖的免疫调节活性及其作用机制[D].南昌大学,2014.
[19] 黄河,姜建民,傅惠英.中国被毛孢发酵物对小鼠T、B淋巴细胞转化功能的影响[J].浙江中医药大学学报,2010,34(4):604-605.
[20] Wu Y,Tian Z,Wei H.Developmental and functional control of natural killer cells by cytokines[J].Front Immunol,2017,8:930.
[2] 梅全喜.广东地产药材研究[M].广州:广东科技出版社,2011:247-250.
[3] 周新兰,何祥久,周光雄,等.龙葵全草皂苷类化学成分研究[J].中草药,2006,37(11):1618-1621.
[4] 赵莹,刘飞,娄红祥.龙葵化学成分研究[J].中药材,2010,33(4):555-556.
[5] Sikdar M,Dutta U.Traditional phytotherapy among the nath people of assam[J].Ethno-Med,2008,2(1):39-45.
[6] Jain R,Sharma A,Gupta S,et al..Solanum nigrum:current perspectives on therapeutic properties[J].Altern Med Rev,2011,16(1):78-85.
[7] 尚庆辉,解玉怀,张桂国,等.植物多糖的免疫调节作用及其机制研究进展[J].动物营养学报,2015,27(1):49-58.
[8] 章世元,徐春燕,董晓芳,等.苜蓿多糖和黄芪多糖对肉仔鸡淋巴细胞增殖的影响[J].动物营养学报,2010,22(3):670-674.
[9] Kim HS,Kim YJ,Lee HK,et al..Activation of macrophages by polysaccharide isolated from Paecilomyces cicadae through toll-like receptor 4[J].Food Chem Toxicol,2012,50(9):3190-3197.
[10] 王淑萍,郭艳平.龙葵多糖提取分离工艺研究[J].信阳师范学院学报:自然科学版,2011,24(2):258-260.
[11] 张淑杰,康玉凡.天然活性多糖研究进展[J].食品工业科技,2017,38(2):378-382,389.
[12] 刘鹭,潘道东,曾小群,等.硒化乳酸菌胞外多糖对体外培养小鼠脾淋巴细胞分泌NO、IL-4及IL-12的影响[J].中国食品学报,2014,14(3):17-21.
[13] 赵磊,王成涛,籍保平.鹿茸水提取物及其蛋白酶解物对小鼠脾淋巴细胞增殖的影响[J].北京工商大学学报:自然科学版,2011,29(3):20-27.
[14] 秦乐,陈新梅,邢佰颖,等.昆仑雪菊总黄酮对小鼠胸腺指数及脾脏指数的影响[J].山东化工,2017,46(15):41-42.
[15] Liu LL,Pfefferle A,Yi SV,et al..Harnessing adaptive natural killer cells in cancer immunotherapy[J].Mol Oncol,2015,9(10):1904-1917.
[16] 张辉,李玉虎.中药复方促使T淋巴细胞增殖的实验研究[J].中国实用医药,2009,4(1):1-2.
[17] Kuang P,Deng H,Cui H,et al..Suppressive effects of sodium fluoride on cultured splenic lymphocyte proliferation in mice[J].Oncotarget,2016,7(38):61905-61915.
[18] 余强.黑灵芝多糖的免疫调节活性及其作用机制[D].南昌大学,2014.
[19] 黄河,姜建民,傅惠英.中国被毛孢发酵物对小鼠T、B淋巴细胞转化功能的影响[J].浙江中医药大学学报,2010,34(4):604-605.
[20] Wu Y,Tian Z,Wei H.Developmental and functional control of natural killer cells by cytokines[J].Front Immunol,2017,8:930.