爬山虎衰老过程中不同颜色叶片光合性能比较
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摘要
爬山虎(Parthenocissus tricuspidatadf Plach.)叶片颜色在秋季变化显著,是研究色素与叶片生理状态之间关系的理想材料。以秋季爬山虎绿色、红色、黄色叶片为材料,测定吸收光谱和叶绿素荧光动力学曲线,以及叶绿素、类胡萝卜素、花色素含量。在3种叶片中,黄色叶片的叶绿素、类胡萝卜素、花色素含量最低,类胡萝卜素含量显著低于花色素含量,可见光吸收最少,活性反应中心数量和光化学活性最低,荧光动力学曲线失去典型特征;红色叶的花色素含量最高,叶绿素含量、类胡萝卜素含量显著高于黄色叶,可见光吸收少于绿色叶,荧光动力学曲线基本上失去典型特征,活性反应中心数量和光化学活性高于黄色叶;绿色叶的叶绿素、类胡萝卜素含量最高,花色素含量高于黄色叶,光吸收光谱和可见光吸收光谱具有正常特征,光化学活性、活性反应中心数量较大。结果表明,绿色叶还具有较强活性氧产生能力,而黄色叶、红色叶的活性氧产生能力较弱,在3种叶片中花色素在活性氧清除方面都起重要作用。
引文
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[24]Gan S,Amasino R M. Making sense of senescence:molecular genetic regulation and manipulation of leaf senescence[J]. Plant Physiology,1997,113(2):313-319.
[25]胡紫菱,崔震海,秦萍,等.深冬季节油松针叶过剩光能的耗散及光氧化防御研究[J].沈阳农业大学学报,2012,43(5):555-559.
[26]Dietz K J,Turkan I,Krieger-Liszkay A. Redox-and reactive oxygen species-dependent signaling into and out of the photosynthesizing chloroplast[J]. Plant Physiology,2016,171(3):1541-1550.
[27]周丹蓉,方智振,廖汝玉,等.李果皮花色素苷、类黄酮和类胡萝卜素含量及抗氧化性研究[J].营养学报,2013,35(6):571-576.
[28]Thurnham D I. Antioxidant vitamins and cancer prevention[J]. J Micronutrient Anal,1990,7:279-299.
[29]Ogawa K,Sakakibara H,Iwata R,et al. Anthocyanin compositian and antioxidant activity of thecrowberry and other berries[J]. J Agric Food Chem,2008,56(12):4457-4462.
[30]Jhin C,Hwang K T. Prediction of radical scavenging activities of anthocyanins applying adaptive neurofuzzy inference system(ANFIS)with quantum chemical descriptors[J]. International Journal of Molecular Sciences,2014,15(8):14715-14727.
[2]孙玉莹,毕京翠,赵志超,等.作物叶片衰老研究进展[J].作物杂志,2013(4):11-19.
[3]晋晓彤.高温、强光及氮营养胁迫对黄瓜幼苗衰老的影响[D].天津:天津大学,2009.
[4]Weng X Y,Xu H X,Yang Y,et al. Water-water cycle involved in dissipation of excess photon energy in phosphorus deficient rice leaves[J]. Biologia Plantarum,2008,52(2):307-313.
[5]张翠利,付丽娜,杨小云,等.活性氧自由基与细胞衰老关系的研究进展[J].广州化工,2015,43(19):5-7.
[6]Miret J A,Munné-Bosch S. Redox signaling and stress tolerance in plants:a focus on vitamin E[J]. Annals of the New York Academy of Sciences,2015,1340(1):29-38.
[7]蒋艾平,刘军,姜景民.檫木叶片秋季衰老时叶色、色素和营养元素的关系[J].林业科学研究,2016,29(3):362-368.
[8]史宝胜,孟建朝,刘冬云,等.秋季不同色泽五叶地锦叶片生理生化特性的研究[J].华北农学报,2009,24(增刊1):172-175.
[9]Asada K. The water-water cycle in chloroplasts:scavenging of active oxygens and dissipation of excess photo[J]. Annual Review Plant Physiology Plant Molecular Biology,1999,50:601-639.
[10]赵文恩,韩雅珊,乔旭光.类胡萝卜素清除活性氧自由基的机理[J].化学通报,1999(4):26-28.
[11]余晶,鲍中英,徐玉敏,等.花青素抗氧化损伤及细胞凋亡的作用研究[J].中西医结合肝病杂志,2009,19(1):24-26.
[12]Ma T,Hu N,Ding C X,et al. In vitro and in vivo biological activities of anthocyanins from Nitraria tangutorun Bobr. fruits[J]. Food Chemistry,2016,194:296-303.
[13]张毅功,陆诗雷,孙振元,等.爬山虎属植物利用研究[J].资源科学,2005,27(5):141-145.
[14]董爱文,向中,李立君,等.爬山虎红色素的定性定量分析[J].无锡轻工大学学报(食品与生物技术),2003,22(6):99-102.
[15]黄秋婵,许元明,韦友欢,等.常见桑科榕属植物叶片类胡萝卜素含量的比较分析[J].中国园艺文摘,2013(6):1-3.
[16]韦友欢,黄秋婵,王慧珏,等.阴生植物与阳生植物色素含量的比较分析[J].湖北农业科学,2010,49(5):1126-1129.
[17]桂克印,文亮晶,李炎林,等.不同离子处理对红檵木叶片色素含量的影响[J].湖北农业科学,2009,48(8):1919-1922.
[18]江力,刘义新,张荣铣.烟叶成熟衰老过程中光合电子传递和光合酶活性变化的研究[J].华中农业大学学报,2000,19(6):533-536.
[19]罗紫东,关华德,章新平,等.枫香叶片衰老过程中光合能力的变化[J].应用生态学报,2016,27(10):3129-3136.
[20]逄好胜,张会慧,田野,等.不同光强下盐胁迫对桑树幼苗叶片活性氧和叶绿素荧光特性的影响[J].中南林业科技大学学报,2014,34(8):42-47.
[21]Zhang A O,Cui Z H,Yu J L,et al. Dissipation of excess excitation energy of the needle leaves in Pinus trees during cold winters[J].International Journal of Biometeorology,2016,60(12):1953-1960.
[22]陈瑾歆,陈建业.自由基与衰老关系的研究进展[J].川北医学院学报,2004,19(1):207-209.
[23]Lee D W,O'Keefe J,Holbrook N M,et al. Pigment dynamics and autumn leaf senescence in a New England deciduous forest,eastern USA[J]. Ecological Research,2003,18(6):677-694.
[24]Gan S,Amasino R M. Making sense of senescence:molecular genetic regulation and manipulation of leaf senescence[J]. Plant Physiology,1997,113(2):313-319.
[25]胡紫菱,崔震海,秦萍,等.深冬季节油松针叶过剩光能的耗散及光氧化防御研究[J].沈阳农业大学学报,2012,43(5):555-559.
[26]Dietz K J,Turkan I,Krieger-Liszkay A. Redox-and reactive oxygen species-dependent signaling into and out of the photosynthesizing chloroplast[J]. Plant Physiology,2016,171(3):1541-1550.
[27]周丹蓉,方智振,廖汝玉,等.李果皮花色素苷、类黄酮和类胡萝卜素含量及抗氧化性研究[J].营养学报,2013,35(6):571-576.
[28]Thurnham D I. Antioxidant vitamins and cancer prevention[J]. J Micronutrient Anal,1990,7:279-299.
[29]Ogawa K,Sakakibara H,Iwata R,et al. Anthocyanin compositian and antioxidant activity of thecrowberry and other berries[J]. J Agric Food Chem,2008,56(12):4457-4462.
[30]Jhin C,Hwang K T. Prediction of radical scavenging activities of anthocyanins applying adaptive neurofuzzy inference system(ANFIS)with quantum chemical descriptors[J]. International Journal of Molecular Sciences,2014,15(8):14715-14727.