天麻不同极性成分的体外抗氧化综合评价
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摘要
通过体外抗氧化活性评价实验,研究天麻6种不同极性成分的抗氧化作用。分别用水和95%的乙醇回流提取天麻块茎,浓缩的乙醇浸膏依次用石油醚、二氯甲烷、乙酸乙酯和正丁醇进行萃取,各极性成分分别配制成适当浓度的溶液,采用1,1-二苯基-2-苦基肼(DPPH)自由基法、2,2-连氮-双-(3-乙基苯并噻唑啉磺酸-6)二铵盐(ABTS)自由基法、邻苯三酚自氧化法和铁氰化钾还原法测定天麻各极性成分的自由基清除能力和还原能力。从天麻中提取的6种极性成分对DPPH自由基的清除能力效果最好的是乙酸乙酯、二氯甲烷和石油醚萃取物,清除率分别为87.58%、83.33%和68.55%;ABTS自由基的清除率6种成分效果均较好,特别是乙酸乙酯萃取物(99.15%)和二氯甲烷萃取物(98.72%)清除率接近于阳性对照V_E;但超氧阴离子自由基的清除能力6个成分均较弱,清除率明显低于阳性对照V_E;而在还原力测定实验中,在高浓度时,石油醚萃取物表现出比V_E更强的还原能力。在天麻的6种不同极性成分中,石油醚萃取物的综合抗氧化活性最强,其APC指数达到了71.82%。
To investigate the antioxidant activity of six different polar extracts form Gastrodia elata Bl. in vitro. Gastrodia elata Bl. was extracted over 2 hours with 10 times of 95% alcohol refluxing for 2 times and the combined alcohol extracts were concentrated under vacuum drying, and the same method to get the water extract. The resultant residue was partitioned in petroleum ether, dichloromethane, ethyl acetate and n-butanol orderly. The four extracts were configured to the appropriate solution. The antioxidant activity of the six extracts were analyzed by 1,1-diphenyl-2-picrylhydrazyl(DPPH) radical scavenging, 2′,2′-azino-bis(3-ethylbenzothiazline-6-sulfonic acid) diammonium salt(ABTS) radical scavenging, superoxide radical scavenging and Fe3+ reduction capacity experiments in vitro. Among the six polar components of Gastrodia elata Bl., the best components of DPPH scavenging ability was ethyl acetate extract, methylene chloride extract and petroleum ether extract with the clearance rates of 87.58% and 83.33%, 68.55% respectively; the six components have better efficiency for ABTS radical scavenging, especially ethyl acetate extract(99.15%) and dichloromethane extract(98.72%) clearance rate close to the positive control V_E; the scavenging capacity of superoxide anion radical was weak and the clearance rate was significantly lower than that of positive control V_E; However, in the determination of reducing power, the petroleum ether extract showed stronger reducing ability than V_E at high concentration. Petroleum ether extract is one of the six polar components of Gastrodia elata Bl., has the highest comprehensive antioxidant activity with APC index of 71.82%.
To investigate the antioxidant activity of six different polar extracts form Gastrodia elata Bl. in vitro. Gastrodia elata Bl. was extracted over 2 hours with 10 times of 95% alcohol refluxing for 2 times and the combined alcohol extracts were concentrated under vacuum drying, and the same method to get the water extract. The resultant residue was partitioned in petroleum ether, dichloromethane, ethyl acetate and n-butanol orderly. The four extracts were configured to the appropriate solution. The antioxidant activity of the six extracts were analyzed by 1,1-diphenyl-2-picrylhydrazyl(DPPH) radical scavenging, 2′,2′-azino-bis(3-ethylbenzothiazline-6-sulfonic acid) diammonium salt(ABTS) radical scavenging, superoxide radical scavenging and Fe3+ reduction capacity experiments in vitro. Among the six polar components of Gastrodia elata Bl., the best components of DPPH scavenging ability was ethyl acetate extract, methylene chloride extract and petroleum ether extract with the clearance rates of 87.58% and 83.33%, 68.55% respectively; the six components have better efficiency for ABTS radical scavenging, especially ethyl acetate extract(99.15%) and dichloromethane extract(98.72%) clearance rate close to the positive control V_E; the scavenging capacity of superoxide anion radical was weak and the clearance rate was significantly lower than that of positive control V_E; However, in the determination of reducing power, the petroleum ether extract showed stronger reducing ability than V_E at high concentration. Petroleum ether extract is one of the six polar components of Gastrodia elata Bl., has the highest comprehensive antioxidant activity with APC index of 71.82%.
引文
[1]于雪,胡文忠,姜爱丽,等.天麻的活性成分及功能性研究进展[J].食品工业科技,2016,37(8):392
[2]Chen P J,Sheen L Y.Gastrodiae Rhizoma(tiān má):a review of biological activity and antidepressant mechanisms[J].J Tradit Complement Med,2011,1(1):31-40
[3]Matias M,Silvestre S,Falc?o A,et al.Gastrodia elata and epilepsy:Rationale and therapeutic potential[J].Phytomedicine,2016,23(12):1511-1526
[4]Zhan H D,Zhou H Y,Sui Y P,et al.The rhizome of Gastrodia elata Blume-An ethnopharmacological review[J].J Ethnopharmacol,2016,189:361-385
[5]段小花,李资磊,杨大松,等.昭通产天麻化学成分研究[J].中药材,2013,36(10):1608-1611
[6]王亚男,林生,陈明华,等.天麻水提取物的化学成分研究[J].中国中药杂志,2012,37(12):1775-1781
[7]申博,高鸿,贾利蓉,等.加工和贮藏条件对天麻提取物抗氧化活性的影响[J].食品科技,2015,40(9):204-208
[8]王庆,李丹丹,潘芸芸,等.提取方法对天麻多糖提取率及其抗氧化活性的影响[J].食品与机械,2017,33(9):146-150
[9]孙海燕.天麻多酚的超声辅助提取工艺优化及抗氧化活性研究[J].食品与机械,2016,32(10):158-163
[10]Franklin G,Concei??o L F,Kombrink E,et al.Xanthone biosynthesis in Hypericum perforatum cells provides antioxidant and antimicrobial protection upon biotic stress[J].Phytochemistry,2009,70(1):60-68
[11]Lou H Q,Hu Y,Zhang L Y,et al.Nondestructive evaluation of the changes of total flavonoid,total phenols,ABTS and DPPH radical scavenging activities,and sugars during mulberry(Morus alba L.)fruits development by chlorophyll fluorescence and RGB intensity values[J].Lwt-Food Sci Technol,2012,47(1):19-24
[12]李楠,师俊玲,王昆.14种海棠果实多酚种类及体外抗氧化活性分析[J].食品科学,2014,35(5):53-58
[13]Gomathi D,Ravikumar G,Kalaiselvi M,et al.In vitro free radical scavenging activity of ethanolic extract of the whole plant of Evolvulus Alsinoides(L.)L[J].Chinese Journal of Integrative Medicine,2015,21(6):453-458
[14]解春艳,秦静,韩爽,等.4种常用食用菌子实体多糖抗氧化活性的比较[J].安徽农业科学,2014,42(35):12643-12645
[15]韩少华,朱靖博,王妍妍.邻苯三酚自氧化法测定抗氧化活性的方法研究[J].中国酿造,2009,28(6):155-157
[16]杨静毅,喻玲玲,吴梅,等.银合欢果皮总黄酮含量测定及抗氧化活性[J].食品科学,2015,36(8):187-190
[17]Seeram N P,Aviram M,Zhang Y J,et al.Comparison of antioxidant potency of commonly consumed polyphenolrich beverages in the United States[J].Journal of Agricultural and Food Chemistry,2008,56(4):1415-1422
[18]王根女.紫苏中酚类物质的微波辅助提取工艺及其抗氧化能力研究[D].杭州:浙江大学,2010
[19]Choi Y,Jeong H S,Lee J.Antioxidant activity of methanolic extracts from some grains consumed in Korea[J].Food Chemistry,2007,103(1):130-138
[20]张华,周志钦,席万鹏.15种柑橘果实主要酚类物质的体外抗氧化活性比较[J].食品科学,2015,36(11):64-70
[2]Chen P J,Sheen L Y.Gastrodiae Rhizoma(tiān má):a review of biological activity and antidepressant mechanisms[J].J Tradit Complement Med,2011,1(1):31-40
[3]Matias M,Silvestre S,Falc?o A,et al.Gastrodia elata and epilepsy:Rationale and therapeutic potential[J].Phytomedicine,2016,23(12):1511-1526
[4]Zhan H D,Zhou H Y,Sui Y P,et al.The rhizome of Gastrodia elata Blume-An ethnopharmacological review[J].J Ethnopharmacol,2016,189:361-385
[5]段小花,李资磊,杨大松,等.昭通产天麻化学成分研究[J].中药材,2013,36(10):1608-1611
[6]王亚男,林生,陈明华,等.天麻水提取物的化学成分研究[J].中国中药杂志,2012,37(12):1775-1781
[7]申博,高鸿,贾利蓉,等.加工和贮藏条件对天麻提取物抗氧化活性的影响[J].食品科技,2015,40(9):204-208
[8]王庆,李丹丹,潘芸芸,等.提取方法对天麻多糖提取率及其抗氧化活性的影响[J].食品与机械,2017,33(9):146-150
[9]孙海燕.天麻多酚的超声辅助提取工艺优化及抗氧化活性研究[J].食品与机械,2016,32(10):158-163
[10]Franklin G,Concei??o L F,Kombrink E,et al.Xanthone biosynthesis in Hypericum perforatum cells provides antioxidant and antimicrobial protection upon biotic stress[J].Phytochemistry,2009,70(1):60-68
[11]Lou H Q,Hu Y,Zhang L Y,et al.Nondestructive evaluation of the changes of total flavonoid,total phenols,ABTS and DPPH radical scavenging activities,and sugars during mulberry(Morus alba L.)fruits development by chlorophyll fluorescence and RGB intensity values[J].Lwt-Food Sci Technol,2012,47(1):19-24
[12]李楠,师俊玲,王昆.14种海棠果实多酚种类及体外抗氧化活性分析[J].食品科学,2014,35(5):53-58
[13]Gomathi D,Ravikumar G,Kalaiselvi M,et al.In vitro free radical scavenging activity of ethanolic extract of the whole plant of Evolvulus Alsinoides(L.)L[J].Chinese Journal of Integrative Medicine,2015,21(6):453-458
[14]解春艳,秦静,韩爽,等.4种常用食用菌子实体多糖抗氧化活性的比较[J].安徽农业科学,2014,42(35):12643-12645
[15]韩少华,朱靖博,王妍妍.邻苯三酚自氧化法测定抗氧化活性的方法研究[J].中国酿造,2009,28(6):155-157
[16]杨静毅,喻玲玲,吴梅,等.银合欢果皮总黄酮含量测定及抗氧化活性[J].食品科学,2015,36(8):187-190
[17]Seeram N P,Aviram M,Zhang Y J,et al.Comparison of antioxidant potency of commonly consumed polyphenolrich beverages in the United States[J].Journal of Agricultural and Food Chemistry,2008,56(4):1415-1422
[18]王根女.紫苏中酚类物质的微波辅助提取工艺及其抗氧化能力研究[D].杭州:浙江大学,2010
[19]Choi Y,Jeong H S,Lee J.Antioxidant activity of methanolic extracts from some grains consumed in Korea[J].Food Chemistry,2007,103(1):130-138
[20]张华,周志钦,席万鹏.15种柑橘果实主要酚类物质的体外抗氧化活性比较[J].食品科学,2015,36(11):64-70