大孔树脂纯化天麻多糖的工艺研究
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摘要
本研究以炮制的干天麻为原料,水提醇沉法提取多糖,大孔吸附树脂纯化,比较了八种大孔树脂(AB-8、D101、LX-17、D301、NKA-9、S-8、LSD-001、ADS-7)对天麻多糖静态吸附-解析效果,筛选出最佳纯化树脂,再研究最佳树脂纯化天麻多糖工艺参数.结果为:八种大孔吸附树脂中D101对天麻多糖的纯化效果最好.样品液浓度、温度、上样速度,洗脱用乙醇浓度、洗脱流速及洗脱体积等因素均对D101树脂吸附分离天麻多糖有影响.所得的最佳纯化工艺为:20℃是较适宜的吸附温度,上样速度1BV/h,上样浓度4mg/mL,进行吸附;吸附饱和平衡后,用解析液浓度60%乙醇,解析速率2BV/h,解析液体积3BV进行动态洗脱.通过该工艺天麻多糖的纯度提高到了65.7%,表明了大孔树脂D101对天麻多糖具有较好的纯化效果.
The Gastrodia elata polysaccharides were purified using a macroporous adsorption resins method.By comparing the Gastrodia elata polysaccharides separation effect of eight different types(AB-8,D101,LX-17,D301,NKA-9,S-8,LSD-001 and ADS-7)of macroporous resin,D101 was selected as the ideal adsorbent as it had a strong adsorption ability and a high desorption rate.The optimal purification technology was as follows:loading rate1 BV/h,loading concentration 4 mg/mL,60%ethanol as suitable elution solvent from the resin,the elution solvent volume 3 BV and the elution flow rate2 BV/h.The purity of Gastrodia elata polysaccharide was increased from 15.6%to 65.7%,which indicated that macroporous resin D101 had better purification effect on Gastrodia elata polysaccharide.
The Gastrodia elata polysaccharides were purified using a macroporous adsorption resins method.By comparing the Gastrodia elata polysaccharides separation effect of eight different types(AB-8,D101,LX-17,D301,NKA-9,S-8,LSD-001 and ADS-7)of macroporous resin,D101 was selected as the ideal adsorbent as it had a strong adsorption ability and a high desorption rate.The optimal purification technology was as follows:loading rate1 BV/h,loading concentration 4 mg/mL,60%ethanol as suitable elution solvent from the resin,the elution solvent volume 3 BV and the elution flow rate2 BV/h.The purity of Gastrodia elata polysaccharide was increased from 15.6%to 65.7%,which indicated that macroporous resin D101 had better purification effect on Gastrodia elata polysaccharide.
引文
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[23]陈可,曲绮雯,陈霞连,等.藏红花花瓣粗多糖的体外抗氧化活性研究[J].四川大学学报:自然科学版,2016,53:448.
[24]王静,雷宏杰,岳珍珍,等.大孔树脂对红枣汁中棒曲霉素的吸附动力学[J].农业工程学报,2015,31:285.
[25]朱建飞,陈玉玲,唐春红.大孔吸附树脂纯化紫苏粕多糖水提液的研究[J].中国油脂,2012,37:63.
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[2]Matias M,Silvestre S,Falc2o A,et al.Gastrodia elata and epilepsy:Rationale and therapeutic potential[J].Phytomedicine,2016,23:1511.
[3]Zhan H D,Zhou H Y,Sui Y P,et al.The rhizome of Gastrodia elata Blume-An ethnopharmacological review[J].J Ethnopharmacol,2016,189:361.
[4]段小花,李资磊,杨大松,等.昭通产天麻化学成分研究[J].中药材,2013,36:1608.
[5]Qiu H,Tang W,Tong X,et al.Structure elucidation and sulfated derivatives preparation of two alpha-D-glucans from Gastrodia elata Bl.and their anti-dengue virus bioactivities[J].Carbohydr Res,2007,342:2230.
[6]Chen X,Cao D,Zhou L,et al.Structure of a polysaccharide from Gastrodia elata Bl.,and oligosaccharides prepared there of with anti-pancreatic cancer cell growth activities[J].Carbohyd Polym,2011,86:1300.
[7]葛进,张磊,刘大会,等.产地、商品级别和干燥工艺对天麻品质影响研究[J].中药材,2017,40:637.
[8]魏士杰,陈文强.云芝子实体多糖对人宫颈癌HeLa细胞生长和凋亡的影响研究[J].四川大学学报:自然科学版,2016,53:1162.
[9]He X,Wang X,Fang J,et al.Polysaccharides in Grifola frondosa mushroom and their health promoting properties:A review[J].Int J Biol Macromol,2017,101:910.
[10]王丽波,程龙,徐雅琴,等.南瓜籽多糖热水提取工艺优化及其抗氧化活性[J].农业工程学报,2016,32:284.
[11]张梦娟,徐怀德,牛素哲.天麻多糖水提工艺优化研究[J].食品工业科技,2006,27:119.
[12]李志英,双少敏,张海容,等.微波法提取天麻多糖的研究[J].山西大学学报:自然科学版,2008,31:573.
[13]朱晓霞,罗学刚.天麻多糖提取工艺优化[J].时珍国医国药,2007,18:906.
[14]杨天友,刘金涛,李刚凤.超声波辅助提取德江天麻多糖工艺优化[J].中国酿造,2015,34:117.
[15]明建,曾凯芳,吴素蕊,等.天麻多糖PGEB-3-H对东莨菪碱所致小鼠学习记忆障碍的影响[J].食品科学,2010,31:246.
[16]周本宏,梁艺小,沈恒,等.天麻多糖对皮质酮损伤PC12细胞保护机制研究[J].中药材,2013,36:630.
[17]王强,张沂,李佳,等.天麻多糖通过影响小鼠免疫系统抑制肿瘤生长[J].免疫学杂志,2014,30:566.
[18]李峰,朱洁平,王艳梅,等.天麻多糖对小鼠免疫性肝损伤的保护作用[J].中药药理与临床,2015,31:111.
[19]李晓冰,展俊平,张月腾,等.天麻多糖对环磷酰胺所致免疫功能低下小鼠体液免疫功能的影响[J].中国老年学杂志,2016,36:1027.
[20]刘明学,李琼芳,刘强,等.天麻多糖分离、结构分析与自由基清除作用研究[J].食品科学,2009,30:29.
[21]张迪,廖剑华,王宏雨.大孔树脂柱吸附纯化双孢蘑菇多糖的工艺[J].福建农业学报,2017,32:175.
[22]陈琛,李鑫鑫,付昀东,等.汉中天麻多糖抗菌活性研究[J].江苏农业科学,2018,46:156.
[23]陈可,曲绮雯,陈霞连,等.藏红花花瓣粗多糖的体外抗氧化活性研究[J].四川大学学报:自然科学版,2016,53:448.
[24]王静,雷宏杰,岳珍珍,等.大孔树脂对红枣汁中棒曲霉素的吸附动力学[J].农业工程学报,2015,31:285.
[25]朱建飞,陈玉玲,唐春红.大孔吸附树脂纯化紫苏粕多糖水提液的研究[J].中国油脂,2012,37:63.
[26]黄民凤.金针菇菌渣多糖的提取纯化及功能评价[D].长沙:湖南农业大学,2015.
[27]马寅达.萝藦总多糖的提取纯化及药理作用的初步研究[J].辽宁医学院,2012,6:21.