GC法测定唇香草挥发油中胡薄荷酮的含量
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摘要
目的采用气相色谱(Gas Chromatography,GC)法测定唇香草挥发油中胡薄荷酮含量。方法色谱柱RTX-5(30cm×0.25mm),程序升温条件:初始温度50℃,保留时间1min;以8℃/min的速率升至210℃,保留时间5min;以20℃/min的速率升至280℃,保留时间3 min,进样量1μL。结果胡薄荷酮浓度在0.57~3.43mg/mL的范围内线性关系良好(r=0.999 8),胡薄荷酮平均回收率为101.7%,RSD=1.7%,唇香草挥发油中胡薄荷酮含量为478.543mg/mL。结论 GC法简便、快速、准确、重现性好,可用于唇香草挥发油中胡薄荷酮的含量测定。
Objective To establish the method for the determination of Hu menthone in Zizphora clinopodioides Lam.volatile oil by gas chromatography(GC).Methods Column RTX-5(30cm×0.25mm)was used,programmed temperature conditions were:initial temperature of 50℃,the retention for1min;at a rate of 8 ℃/min to 210℃,the retention for 5min;at a rate of 20℃/min to 280℃,the retention time for 3min,sample volume of 1μL.Results Concentration of Hu menthone was in the range of0.57~3.43mg/mL where there was a good internal relationship(r =0.999 8),the average recovery was101.7% and RSD was 1.7%,the content of Hu menthol was 478.543 mg/mL in the Zizphora clinopodioides Lam.Conclusion The method we established was simple,rapid,accurate,and reproducible and it can be used to determine the content of Hu menthol in volatile oil from Zizphora clinopodioides Lam.
Objective To establish the method for the determination of Hu menthone in Zizphora clinopodioides Lam.volatile oil by gas chromatography(GC).Methods Column RTX-5(30cm×0.25mm)was used,programmed temperature conditions were:initial temperature of 50℃,the retention for1min;at a rate of 8 ℃/min to 210℃,the retention for 5min;at a rate of 20℃/min to 280℃,the retention time for 3min,sample volume of 1μL.Results Concentration of Hu menthone was in the range of0.57~3.43mg/mL where there was a good internal relationship(r =0.999 8),the average recovery was101.7% and RSD was 1.7%,the content of Hu menthol was 478.543 mg/mL in the Zizphora clinopodioides Lam.Conclusion The method we established was simple,rapid,accurate,and reproducible and it can be used to determine the content of Hu menthol in volatile oil from Zizphora clinopodioides Lam.
引文
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[8]孙文君,于生,单鸣秋,等.GC-MS法测定荆芥挥发油中薄荷酮、胡薄荷酮含量[J].药学与临床研究,2011,19(6):571-572.
[2]陈希元,于晖.唇香草抗心肌缺血药理作用得研究[J].新疆药讯,1982,6(2):66-70.
[3]李雪,张正方,唐军.唇香草挥发油的气相色谱-质谱分析[J].质谱学报,2008,29(2):105-109.
[4]郭玉婷,兰卫,吴燕妮,等.唇香草挥发油对自发性高血压大鼠降压作用的研究[J].新疆医科大学学报,2014,24(3):257-260.
[5]郭沛鑫,解宇环,敖丽,等.胡薄荷酮对LPS致急性肺损伤大鼠肺组织NF-κB、MyD88含量的影响[J].天然产物研究与开发,2013,25(11):1503-1506.
[6]汤奇.荆芥挥发油与主要成分抗流感病毒作用机器研究机制[D].成都:成都中医药大学,2012.
[7]唐杨,颜挺,晁若冰.GC测定荆芥及荆芥油中的薄荷酮和胡薄荷酮[J].华西药学杂志,2008,23(1):102-105.
[8]孙文君,于生,单鸣秋,等.GC-MS法测定荆芥挥发油中薄荷酮、胡薄荷酮含量[J].药学与临床研究,2011,19(6):571-572.