荆芥汤中荆芥最佳煎煮时间的实验研究
|
摘要
目的:通过研究荆芥后下对荆芥汤中胡薄荷酮含量及镇咳、镇痛、免疫功能的影响,探讨荆芥的最佳煎煮时间,为荆芥特殊煎煮方法的临床应用提供理论依据。方法:按照《中国药典》(2015年版)中荆芥含量测定要求测定胡薄荷酮含量;采用致咳、致痛法观察荆芥后下对小鼠镇咳、镇痛作用的影响,通过分析小鼠CD4~+、CD8~+、IL-1、IL-6水平,探讨荆芥后下对小鼠免疫功能的影响。结果:荆芥煎煮7.5 min时,荆芥汤中胡薄荷酮含量最高,与空白组比较其能够明显改善小鼠痛阈值、咳嗽潜伏期及咳嗽次数(P<0.01),提高小鼠CD4~+、CD8~+、IL-1、IL-6水平(P<0.05)。结论:荆芥后下影响荆芥汤中胡薄荷酮含量及治疗效果,荆芥入解表类汤剂宜采用"后下",最佳时间为5～10 min。
OBJECTIVE To investigate the best decoction time of Schizonepetaby studying the effect of Schizonepetalaterdecoction on the content of pulegone in Schizonepetadecoction and its antitussive,analgesic and immune functions,provide a theoretical basis for the clinical application of Schizonepetadecoction.METHODS Content of menthol was determined in accordance with the determination of pulegone in Schizonepetain the 2015 edition of the Chinese Pharmacopoeia.The effects of Schizonepetaon the antitussive and analgesic effects of mice were observed by means of cough and analgesia.By analyzing the levels of CD4~+,CD8~+,IL-1 and IL-6 in mice,the effects of Schizonepetaon the immune function of mice were investigated.RESULTS The content of puergonone in Schizonepeta decoction was the highest at 7.5 min.Compared with the control group,it significantly improved the mice pain threshold,cough latency and cough frequency(P<0.01),increased levels of CD4~+,CD8~+,IL-1,and IL-6 in mice(P<0.05).CONCLUSION After the Schizonepeta affects the menthol content and therapeutic effect of Schizonepetadecoction,it is recommended to use "later-decoction" for the treatment of Schizonepeta,and the best time is 5-10 min.
OBJECTIVE To investigate the best decoction time of Schizonepetaby studying the effect of Schizonepetalaterdecoction on the content of pulegone in Schizonepetadecoction and its antitussive,analgesic and immune functions,provide a theoretical basis for the clinical application of Schizonepetadecoction.METHODS Content of menthol was determined in accordance with the determination of pulegone in Schizonepetain the 2015 edition of the Chinese Pharmacopoeia.The effects of Schizonepetaon the antitussive and analgesic effects of mice were observed by means of cough and analgesia.By analyzing the levels of CD4~+,CD8~+,IL-1 and IL-6 in mice,the effects of Schizonepetaon the immune function of mice were investigated.RESULTS The content of puergonone in Schizonepeta decoction was the highest at 7.5 min.Compared with the control group,it significantly improved the mice pain threshold,cough latency and cough frequency(P<0.01),increased levels of CD4~+,CD8~+,IL-1,and IL-6 in mice(P<0.05).CONCLUSION After the Schizonepeta affects the menthol content and therapeutic effect of Schizonepetadecoction,it is recommended to use "later-decoction" for the treatment of Schizonepeta,and the best time is 5-10 min.
引文
[1]Xu LT,Liu Y.Treatise on Originand Development of Medicine(医学源流论)[M].Beijing:China Press of Traditional Chinese Medicine,2008:43.
[2]Chen C,Pei ZY,Peng JZ,et al.Taiping Huimin Heji Ju Fang;Prescriptions of the Bureau of Taiping People's Welfare Pharmacy(太平惠民和剂局方)[M].Shen Yang:Liaoning Science and Technology Press,1997:74.
[3]Ch P.VolⅠ(中国药典.一部)[S].2015:232.
[4]Tang SW,Wu SZ.Zhenglei Bencao.Classified Materia Medica(证类本草)[M].Beijing:China Medical Science Press,2011:645.
[5]Huang XW,Liu YX,Yang MH,et al.Advances of chemical composition and pharmacological action of Catnip[J].Jilin JChin Med(吉林中医药),2017,37(08):817-819.
[6]Huang XW,Yang MH,Liu YX,et al.Effect of decocting time on the content of pulegone in Nepeta[J].Jilin J Chin Med(吉林中医药),2017,37(09):949-951.
[7]Han D,Sun LX.Study on Pharmacodynamics of Baijing Kangzhi Granule[J].Chin J Pharm Economic(中国药物经济学),2014,9(01):39-40.
[8]LiM,Shen JY,Li TX.Anti-inflammatory and analgesic actions of Xanthii Fructus and its processed product[J].Chin Hosp Pharm J(中国医院药学杂志),2017,37(03):232-234.
[9]Zhao YR.The determination method of high performance liquid and the effect of changes of pulegone content of Schizonepeta pieces in the decocting process of chromatography[J].JNorth Pharm(北方药学),2017,14(10):1-2.
[10]Zhang ZQ,Li ZG,Tong JB,et al.Research Progress on Cough Variant Asthma Treated by Zhisou Powder[J].J Hubei Univ Chin Med(湖北中医药大学学报),2018,20(03):118-121.
[11]Zhao YJ.Determination of the content of menthol in the process of decoction of Schizonepeta chinensis by high performance liquid chromatography[J].Chin J Pharm Economic(中国药物经济学),2016,11(11):21-23.
[12]Medical institution Chinese medicine decocting room management norms[Z].Beijing:State Administration of Traditional Chinese Medicine,2009.
[13]Wang B,Mao Y,Yang M.Efficacy enhancing and toxicity reducing mechanism of astragalus polysaccharides on doxorubicin therapy against mouse mammary cancer[J].Chin Hosp Pharm J(中国医院药学杂志),2017,37(06):497-501.
[14]Xu W.Effects of long-term moderate and small exercise exercise on IL-6 and TNF-αin peripheral blood of rats[J].Chin JRehabilitation Med(中国康复医学杂志),2017,27(9):837-838.
[15]Ge ZY,Tong J,Zhu WX,et al.Study on Effect of Qinggan Ningfei Decoction on CVA Rat's IgE、IL-6、ICAM-1[J].Chin Archives Tradit Chin Med(中华中医药学刊),2012,30(03):596-598.
[2]Chen C,Pei ZY,Peng JZ,et al.Taiping Huimin Heji Ju Fang;Prescriptions of the Bureau of Taiping People's Welfare Pharmacy(太平惠民和剂局方)[M].Shen Yang:Liaoning Science and Technology Press,1997:74.
[3]Ch P.VolⅠ(中国药典.一部)[S].2015:232.
[4]Tang SW,Wu SZ.Zhenglei Bencao.Classified Materia Medica(证类本草)[M].Beijing:China Medical Science Press,2011:645.
[5]Huang XW,Liu YX,Yang MH,et al.Advances of chemical composition and pharmacological action of Catnip[J].Jilin JChin Med(吉林中医药),2017,37(08):817-819.
[6]Huang XW,Yang MH,Liu YX,et al.Effect of decocting time on the content of pulegone in Nepeta[J].Jilin J Chin Med(吉林中医药),2017,37(09):949-951.
[7]Han D,Sun LX.Study on Pharmacodynamics of Baijing Kangzhi Granule[J].Chin J Pharm Economic(中国药物经济学),2014,9(01):39-40.
[8]LiM,Shen JY,Li TX.Anti-inflammatory and analgesic actions of Xanthii Fructus and its processed product[J].Chin Hosp Pharm J(中国医院药学杂志),2017,37(03):232-234.
[9]Zhao YR.The determination method of high performance liquid and the effect of changes of pulegone content of Schizonepeta pieces in the decocting process of chromatography[J].JNorth Pharm(北方药学),2017,14(10):1-2.
[10]Zhang ZQ,Li ZG,Tong JB,et al.Research Progress on Cough Variant Asthma Treated by Zhisou Powder[J].J Hubei Univ Chin Med(湖北中医药大学学报),2018,20(03):118-121.
[11]Zhao YJ.Determination of the content of menthol in the process of decoction of Schizonepeta chinensis by high performance liquid chromatography[J].Chin J Pharm Economic(中国药物经济学),2016,11(11):21-23.
[12]Medical institution Chinese medicine decocting room management norms[Z].Beijing:State Administration of Traditional Chinese Medicine,2009.
[13]Wang B,Mao Y,Yang M.Efficacy enhancing and toxicity reducing mechanism of astragalus polysaccharides on doxorubicin therapy against mouse mammary cancer[J].Chin Hosp Pharm J(中国医院药学杂志),2017,37(06):497-501.
[14]Xu W.Effects of long-term moderate and small exercise exercise on IL-6 and TNF-αin peripheral blood of rats[J].Chin JRehabilitation Med(中国康复医学杂志),2017,27(9):837-838.
[15]Ge ZY,Tong J,Zhu WX,et al.Study on Effect of Qinggan Ningfei Decoction on CVA Rat's IgE、IL-6、ICAM-1[J].Chin Archives Tradit Chin Med(中华中医药学刊),2012,30(03):596-598.