茶多酚预防吗啡所致便秘的效果及机制
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摘要
目的探讨茶多酚对吗啡所致便秘的预防作用及其作用机制。方法雌性昆明种小鼠按随机数字表法分为空白对照组、模型对照组、茶多酚组、茶多酚+吗啡组,每组10只。茶多酚组和茶多酚+吗啡组小鼠灌胃给予茶多酚100 mg·kg~(-1),空白对照组和模型对照组给予0.5%羧甲基纤维素钠溶液0.1 m L·kg~(-1),连续4 d。给药第4天,模型对照组和茶多酚+吗啡组小鼠腹腔注射吗啡20 mg·kg~(-1),茶多酚组和空白对照组腹腔注射0.9%氯化钠溶液0.1 m L·kg~(-1),15 min后小鼠灌胃给予5%墨汁液0.2 m L。热板法检测各组小鼠的第1次舔足时间,观察茶多酚对吗啡镇痛效果的影响;酶联免疫吸附测定(ELISA)法检测各组小鼠小肠内胃动素、P-物质及生长抑素的含量。结果与空白对照组比较,模型对照组墨汁推进长度和墨汁推进率明显降低(P<0.01);茶多酚组墨汁液推进长度和墨汁推进率显著增加(P<0.05)。与模型对照组比较,茶多酚+吗啡组墨汁推进长度和墨汁推进率显著升高(P<0.01)。空白对照组和茶多酚组小鼠第1次舔足时间分别为(8.64±2.72),(9.11±2.13)s,模型对照组和茶多酚+吗啡组小鼠第1次舔足时间分别为(18.79±3.58),(20.10±3.72)s。与空白对照组比较,模型对照组胃动素、P物质含量减少(P<0.05),茶多酚组胃动素、P物质明显增加(P<0.05);与模型对照组比较,茶多酚+吗啡组胃动素、P物质含量明显增加(P<0.05)。与空白对照组比较,模型对照组生长抑素含量增加,而茶多酚组和茶多酚+吗啡组生长抑素含量显著减少(P<0.05)。结论茶多酚在不降低疼痛治疗效果的前提下,能有效改善小鼠肠蠕动预防吗啡所致便秘,这一作用与茶多酚对肠道胃动素、P物质和生长抑素的含量调节有关。
Objective To explore the preventive effect and mechanism of tea polyphenols on morphine-induced constipation. Methods Female Kunming mice were randomly divided into 4 groups( 10 mice per group),including blank control group,model control group,tea polyphenols group and tea polyphenols + morphine group. Tea polyphenols group and tea polyphenols + morphine group were pretreated with 100 mg·kg~(-1) of tea polyphenols for 4 days,meanwhile blank control group and model control group were preteated with 0.1 m L·kg~(-1) of 0.5% CMC-Na for 4 days. On the fourth day model control group and tea polyphenols + morphine group were intraperitoneal injected 20 mg kg~(-1) morphine,otherwise blank control group and tea polyphenols group were injected with 0.1 m L·kg~(-1) of 0.9% sodium chloride solution. Then mice were given 0.2 m L of 5% ink solution by intragastric administration 15 min later. The latency to paw licking was detected in hot plate test to evaluate the effect of tea polyphenols on morphine analgesia. The levele of motilin( MLT),substance P( SP) and somatostatin( SS) in intestinal tissue were determined by enzyme-linked immunosorbent assay( ELISA) among groups. Results Compared with the blank control group,the length of propelling ink and the propelling rate of ink were significantly lower in the model control group( P<0.01),meanwhile tea polyphenols group were much higher( P < 0. 05). Compared with model control group,the length of propelling ink and the propelling rate of ink were significantly higher in tea polyphenols + morphine group mice( P<0.05). The first paw licking time of control group and tea polyphenols group were( 8. 64 + 2. 72) s and( 9. 11 + 2. 13) s,and the time of model control group and tea polyphenols + morphine group were( 18.79±3.58) s and( 20.10±3.72) s. The contents of MLT and SP were reduced in model control group( P < 0. 05),but significantly increased in tea polyphenols group( P < 0. 05) compared with blank control group. Compared with the model control group,MLT and SP had an obviously increase in tea polyphenols +morphine group( P< 0. 05). Compared with blank control group,the content of SS was increased in model control group,but decreased markedly in tea polyphenols group and tea polyphenols + morphine group( P<0.05). Conclusion Tea polyphenols can prevent the morphine-induced constipation without decreasing the analgesic effect of morphine,which is related to the regulation of the content of MLT,SP and SS.
Objective To explore the preventive effect and mechanism of tea polyphenols on morphine-induced constipation. Methods Female Kunming mice were randomly divided into 4 groups( 10 mice per group),including blank control group,model control group,tea polyphenols group and tea polyphenols + morphine group. Tea polyphenols group and tea polyphenols + morphine group were pretreated with 100 mg·kg~(-1) of tea polyphenols for 4 days,meanwhile blank control group and model control group were preteated with 0.1 m L·kg~(-1) of 0.5% CMC-Na for 4 days. On the fourth day model control group and tea polyphenols + morphine group were intraperitoneal injected 20 mg kg~(-1) morphine,otherwise blank control group and tea polyphenols group were injected with 0.1 m L·kg~(-1) of 0.9% sodium chloride solution. Then mice were given 0.2 m L of 5% ink solution by intragastric administration 15 min later. The latency to paw licking was detected in hot plate test to evaluate the effect of tea polyphenols on morphine analgesia. The levele of motilin( MLT),substance P( SP) and somatostatin( SS) in intestinal tissue were determined by enzyme-linked immunosorbent assay( ELISA) among groups. Results Compared with the blank control group,the length of propelling ink and the propelling rate of ink were significantly lower in the model control group( P<0.01),meanwhile tea polyphenols group were much higher( P < 0. 05). Compared with model control group,the length of propelling ink and the propelling rate of ink were significantly higher in tea polyphenols + morphine group mice( P<0.05). The first paw licking time of control group and tea polyphenols group were( 8. 64 + 2. 72) s and( 9. 11 + 2. 13) s,and the time of model control group and tea polyphenols + morphine group were( 18.79±3.58) s and( 20.10±3.72) s. The contents of MLT and SP were reduced in model control group( P < 0. 05),but significantly increased in tea polyphenols group( P < 0. 05) compared with blank control group. Compared with the model control group,MLT and SP had an obviously increase in tea polyphenols +morphine group( P< 0. 05). Compared with blank control group,the content of SS was increased in model control group,but decreased markedly in tea polyphenols group and tea polyphenols + morphine group( P<0.05). Conclusion Tea polyphenols can prevent the morphine-induced constipation without decreasing the analgesic effect of morphine,which is related to the regulation of the content of MLT,SP and SS.
引文
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[12]王胜红.关于茶多酚的研究情况[J].中国中医药资讯,2011,3(10):68.
[13]GEORGIADES P,PUDNEY P D,ROGERS S,et al.Tea derived galloylated polyphenols cross-link purified gastrointestinal mucins[J].PLo S One,2014,9(8):e105302.
[14]VODNAR D C.Green tea increases the survival yield of bifidobacteria in simulated gastrointestinal environment and during refrigerated conditions[J].Chem Central J,2012,6(1):61.
[15]吴香兰.黑茶改善小鼠胃肠道功能的实验研究[D].长沙:湖南农业大学,2013.
[16]CHEN C Y,TSAI C Y.Ghrelin and motilin in the gastrointestinal system[J].Curr Pharm Design,2012,18(31):4755-4765.
[17]HOOGERWERF W A,SARNA S K.Tachykinin receptors as drug targets for motility disorders[J].Dig Dis,2006,24(1/2):83-90.
[18]CHAUDHURI L,BASU S,SETH P,et al.Prokinetic effect of black tea on gastrointestinal motility[J].Life Sci,2000,66(9):847-854.
[2]叶嵩,郭文俊.阿片类药物所致便秘的产生机制及治疗[J].长治医学院学报,2015,29(2):149-151.
[3]杨晓萍,覃筱燕.茶多酚药理活性的研究进展[J].中央民族大学学报(自然科学版),2013,22(3):24-28.
[4]AFZAL M,SAFER A M,MENON M.Green tea polyphenols and their potential role in health and disease[J].Inflammopharmacology,2015,23(4):151-161.
[5]王栋,康健.茶多酚的功效、提取和应用前景[J].新疆大学学报(自然科学版),2007,24(2):217.
[6]胡逸君,竹剑平.茶多酚治疗产后便秘52例临床疗效观察[J].海峡药学,2009,21(6):162-163.
[7]KON R,IKARASHI N,HAYAKAWA A,et al.Morphineinduced constipation develops with increased aquaporin-3expression in the colon via increased serotonin secretion[J].Toxicol Sci,2015,145(2):337-347.
[8]医学名词审定委员会.中国工具书网络出版总库[EB/OL].[2014-07-01].http://gongjushu.cnki.net/refbook/basicsearch.aspx?kw=吗啡.
[9]许钦燕,王国英.电针治疗吗啡所致便秘40例临床观察[J].河北中医,2013,35(12):1845-1846.
[10]叶富英,汪永坚.复方大黄膏敷脐结合按摩治疗吗啡致便秘50例[J].中国中医药科技,2013,20(2):150.
[11]姚兰,叶序卷,贾钰铭,等.番泻叶与茶叶浸出液联合治疗盐酸吗啡缓释片所致便秘的临床观察[J].中国医院用药评价与分析,2015,15(6):716-718.
[12]王胜红.关于茶多酚的研究情况[J].中国中医药资讯,2011,3(10):68.
[13]GEORGIADES P,PUDNEY P D,ROGERS S,et al.Tea derived galloylated polyphenols cross-link purified gastrointestinal mucins[J].PLo S One,2014,9(8):e105302.
[14]VODNAR D C.Green tea increases the survival yield of bifidobacteria in simulated gastrointestinal environment and during refrigerated conditions[J].Chem Central J,2012,6(1):61.
[15]吴香兰.黑茶改善小鼠胃肠道功能的实验研究[D].长沙:湖南农业大学,2013.
[16]CHEN C Y,TSAI C Y.Ghrelin and motilin in the gastrointestinal system[J].Curr Pharm Design,2012,18(31):4755-4765.
[17]HOOGERWERF W A,SARNA S K.Tachykinin receptors as drug targets for motility disorders[J].Dig Dis,2006,24(1/2):83-90.
[18]CHAUDHURI L,BASU S,SETH P,et al.Prokinetic effect of black tea on gastrointestinal motility[J].Life Sci,2000,66(9):847-854.