青蒿琥酯对糖尿病大鼠伤口皮肤TNF-α的影响
|
摘要
目的建立糖尿病大鼠模型后,背部皮肤制造伤口,局部涂抹0.5%青蒿琥酯(ART)干预,观察药效并免疫组化法检测伤口皮肤肿瘤坏死因子-α(TNF-α)的影响。方法选取SD大鼠20只,随机分为糖尿病盐水组、糖尿病ART组,适应性喂养后糖尿病组大鼠注射链脲佐菌素(STZ)诱导1型糖尿病,监测血糖,成功建模2周后,所有大鼠背部皮肤制造伤口,用药2周后处死,取背部皮肤行免疫组织化学法观察皮肤TNF-α变化。结果注射STZ后成功建立糖尿病大鼠模型,免疫组化提示糖尿病ART组伤口皮肤中TNF-α表达较糖尿病盐水组显著下降(P<0.05)。结论 ART能下降TNF-α的表达,通过抗炎作用,促进伤口愈合,为糖尿病皮肤伤口研究提供一种新的药物治疗方案。
Objective To establish a diabetic rat model,the wounds were made on the back skin,and the0.5% artesunate(ART) was applied.The effects of the drug and the tumor necrosis factor-α(TNF-α) expression in skin wounds were observed.Methods 20 SD rats were randomly divided into diabetic saline group and diabetic ART group.Diabetic rats were injected with streptozotocin(STZ) to induce type 1 diabetes,and blood glucose was monitored.After 2 weeks,wounds were made on the back skin of all rats.After 2 weeks of treatment,the rats were sacri?ced.The effects of ART and the tumor necrosis factor-α(TNF-α) expression in skin wounds were observed.Results The diabetic rat model was successfully established.The expression of TNF-α in the diabetic ART group was significantly lower than that in the diabetic saline group(P<0.05).Conclusion ART cound exert antiin? ammatory effect and be good for wound healing by decreasing the expression of TNF-α,and this provides a new drug treatment plan for diabetic skin wound research.
Objective To establish a diabetic rat model,the wounds were made on the back skin,and the0.5% artesunate(ART) was applied.The effects of the drug and the tumor necrosis factor-α(TNF-α) expression in skin wounds were observed.Methods 20 SD rats were randomly divided into diabetic saline group and diabetic ART group.Diabetic rats were injected with streptozotocin(STZ) to induce type 1 diabetes,and blood glucose was monitored.After 2 weeks,wounds were made on the back skin of all rats.After 2 weeks of treatment,the rats were sacri?ced.The effects of ART and the tumor necrosis factor-α(TNF-α) expression in skin wounds were observed.Results The diabetic rat model was successfully established.The expression of TNF-α in the diabetic ART group was significantly lower than that in the diabetic saline group(P<0.05).Conclusion ART cound exert antiin? ammatory effect and be good for wound healing by decreasing the expression of TNF-α,and this provides a new drug treatment plan for diabetic skin wound research.
引文
[1]Li G,Zou X,Zhu Y,et al.Expression and infl uence of matrix metalloproteinase-9/tissue inhibitor of metalloproteinase-1and vascular endothelial growth factor in diabetic foot ulcers.Int J Low Extrem Wounds 2017;16:6-13.
[2]Kant V,Gopal A,Pathak NN,et al.Antioxidant and antiinflammatory potential of curcumin accelerated the cutaneous wound healing in streptozotocin-induced diabetic rats.Int Immunopharmacol 2014;20:322-330.
[3]Kasiewicz LN.Recent advances in biomaterials for the treatment of diabetic foot ulcers.Biomater Sci 2017.
[4]朱万博.糖尿病患者创面难愈机制及治疗研究进展.中国糖尿病杂志2016,24(12):1135-1139.
[5]Meisel JE.Selective small-molecule inhibitors as chemical tools to define the roles of matrix metalloproteinases in disease.Biochim Biophys Acta 2017.
[6]Kasiewicz LN,Whitehead KA.Lipid nanoparticles silence tumor necrosis factorαto improve wound healing in diabetic mice.Bioeng Transl Med 2019;4:75-82.
[7]Li Z,Shi X,Liu J,et al.Artesunate prevents type 1 diabetes in NOD mice mainly by inducing protective IL-4-producing Tcells and regulatory T cells.FASEB J 2019.
[8]Gugliandolo E,D'Amico R,Cordaro M,et al.Neuroprotective Effect of Artesunate in Experimental Model of Traumatic Brain Injury.Front Neurol 2018;9:590.
[2]Kant V,Gopal A,Pathak NN,et al.Antioxidant and antiinflammatory potential of curcumin accelerated the cutaneous wound healing in streptozotocin-induced diabetic rats.Int Immunopharmacol 2014;20:322-330.
[3]Kasiewicz LN.Recent advances in biomaterials for the treatment of diabetic foot ulcers.Biomater Sci 2017.
[4]朱万博.糖尿病患者创面难愈机制及治疗研究进展.中国糖尿病杂志2016,24(12):1135-1139.
[5]Meisel JE.Selective small-molecule inhibitors as chemical tools to define the roles of matrix metalloproteinases in disease.Biochim Biophys Acta 2017.
[6]Kasiewicz LN,Whitehead KA.Lipid nanoparticles silence tumor necrosis factorαto improve wound healing in diabetic mice.Bioeng Transl Med 2019;4:75-82.
[7]Li Z,Shi X,Liu J,et al.Artesunate prevents type 1 diabetes in NOD mice mainly by inducing protective IL-4-producing Tcells and regulatory T cells.FASEB J 2019.
[8]Gugliandolo E,D'Amico R,Cordaro M,et al.Neuroprotective Effect of Artesunate in Experimental Model of Traumatic Brain Injury.Front Neurol 2018;9:590.