白介素-1β在大鼠皮肤创伤后不同时段的表达
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摘要
目的观察白介素-1β(IL-1β)在大鼠皮肤创伤后的表达变化。方法 15只SD(Sprague-Dawley)大鼠随机分为1d,3d,7d组,每组5只,采用全层皮肤切除制作创伤修复动物模型,造模后测量伤口未愈面积,并通过HE染色和免疫组织化学染色方法观察损伤区域不同时间段的组织病理学改变和IL-1β的表达变化。结果大鼠皮肤创伤后1d、3d、7d大鼠创伤皮肤未愈合面积均逐渐缩小,3d后的创面修复速率(79%)明显高于3d前的修复速率(2%)。IL-1β在动物上皮组织、巨噬细胞、成纤维细胞中有表达,阳性反应物主要定位在胞核、胞浆和基质中。创伤后IL-1β表达逐渐增强,3d达到高峰值,随后表达逐渐减弱。损伤后3d组IL-1β表达强度高于1d、7d组(P <0.05)。结论大鼠创伤皮肤修复模型诱导了IL-1β的表达,IL-1β在修复过程中发挥着重要作用。
Objective To To observe the change of interleukin-1β(IL-1β) expression in skin wound repair model of rat.Methods15 SD(Sprague-Dawley) rats were randomly divided into 1 d, 3 d and 7 d groups, 5 rats in each group, all rats were subjected to the full-thickness skin excision.The wound unrepaired area was measurement of all the animal models, and the pathologicchange and the expression change of IL-1β were observed by HE staining and immunohistochemistry.Results Determination of unrepaired area of skin wound after injury 1 d, 3 d, 7 d was gradually reduced, and the recovery rate(79%) after 3 days was significantly higher than that before 3 d(2%).IL-1β is expressed in animal epithelial tissue, macrophages and fibroblasts. The positive reactants mainly locate in nucleus, cytoplasm and matrix.The expression of IL-1β gradually increased after trauma and peaked at 3 d, then gradually weakened.The intensity of IL-1β in the 3 d group after injury was higher than that in the 1 st and 7 th day(P < 0.05).Conclusion Rat traumatic skin repair model induced IL-1β expression, IL-1β plays an important role in the repair process.
Objective To To observe the change of interleukin-1β(IL-1β) expression in skin wound repair model of rat.Methods15 SD(Sprague-Dawley) rats were randomly divided into 1 d, 3 d and 7 d groups, 5 rats in each group, all rats were subjected to the full-thickness skin excision.The wound unrepaired area was measurement of all the animal models, and the pathologicchange and the expression change of IL-1β were observed by HE staining and immunohistochemistry.Results Determination of unrepaired area of skin wound after injury 1 d, 3 d, 7 d was gradually reduced, and the recovery rate(79%) after 3 days was significantly higher than that before 3 d(2%).IL-1β is expressed in animal epithelial tissue, macrophages and fibroblasts. The positive reactants mainly locate in nucleus, cytoplasm and matrix.The expression of IL-1β gradually increased after trauma and peaked at 3 d, then gradually weakened.The intensity of IL-1β in the 3 d group after injury was higher than that in the 1 st and 7 th day(P < 0.05).Conclusion Rat traumatic skin repair model induced IL-1β expression, IL-1β plays an important role in the repair process.
引文
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[13]陈先玖.舒筋活血汤对慢性创伤性膝关节滑膜炎的临床疗效及对TNF-α、IL-1β因子的影响[J].中国医药导刊,2017,19(06):582-583.DOI:10.3969/j.issn.1009-0959.2017.06.022
[14]朱晓燕,彭成,钟振东,等.香连金黄散外治阳证疮疡对皮肤炎症微环境影响的实验研究[J].时珍国医国药,2016,27(07):1540-1543.DOI:10.3969/j.issn.1008-0805.2016.07.002
[15]李凤蕾,杜菲,吴迪,等.乌灵菌粉早期应用对创伤后应激障碍模型大鼠行为及海马组织中IL-1β、IL-6水平的影响[J].中国药房,,2016,27(25):3478-3480.DOI:10.6039/j.issn.1001-0408.2016.25.07
[16]张莉,杜冠华.炎性细胞因子抑制剂高通量筛选方法的研究[J].中国药理学通报,2003,(11):1260-1263.DOI:10.3321/j.issn:1001-1978.2003.11.015
[2]蔡源源,李光早.血管内皮生长因子的调控及其作用研究进展[J].组织工程与重建外科杂志,2011,7(1):51-54.DOI:10.3969/j.issn.1673-0364.2011.01.015
[3]丁宁,崔芳芳,邹检平.血管内皮生长因子及其功能[J].中国计划生育学杂志,2011,19(03):189-191.DOI:10.3969/j.issn.1004-8189.2011.03.021
[4]石磊.白介素1与幽门螺旋杆菌研究进展[J].河北医学,2010,16(10):1252-1254.DOI:10.3969/j.issn 1006-6233 2010.10.042
[5]薛盛中,刘保健,董万涛,等.皮肤创伤愈合的分子机制研究进展[J].中医临床研究,2014,6(23):139-142.DOI:10.3969/j.issn.1674-7860.2014.23.085
[6]李晓康,王舒,于杨,等.皮肤创伤修复研究进展[J].中国中西医结合皮肤性病学杂志,2016,15(01):62-65.DOI:10.3969/j.issn.1672-0709.2016.01.026
[7]Demidova-Rice TN,Hamblin MR,Herman IM,etal.Acute and impaired wound healing:pathophysiology and current methods for drug delivery,part1:normal and chronic wounds:biology,causes,and approaches to care[J].Adv Skin Wound Care,2012,25:304-314.DOI:10.1097/01.ASW.0000416006.55218.d0.
[8]Honnegowda TM,Kumar P,Kumar S,etal.Role of angiogenesis and angiogenic factors in acute and chronic wound healing[J].Plast Aesthet Res,2015,2:243-249.DOI:10.4103/2347-9264.165438
[9]Dinarcllo C A,Rosenwasser L J,Wollff S M,etal.Demonstration of a circulating suppressor factor of thymocyte proliferation during endotoxin fever in humans[J].J immunol,1981,127(6):2517-9
[10]Garlanda C,Dinarello C A,Mantovani A,etal.The interleukin-1 family:back to the future[J].Immunity,2013,39(6):1003-18.DOI:10.1016/j.immuni.2013.11.010.
[11]郭甫坤,吴曙光,白介素1信号转导研究进展.国外医学(分子生物学分册),13-15(2001).DOI:10.3870/j.issn.1672-8009.2001.01.005
[12]乐海浪,罗国强.创伤后早期炎症因子TNF-a、IL-1、IL-6的研究进展[J].现代诊断与治疗,2014,25(04):763-765.
[13]陈先玖.舒筋活血汤对慢性创伤性膝关节滑膜炎的临床疗效及对TNF-α、IL-1β因子的影响[J].中国医药导刊,2017,19(06):582-583.DOI:10.3969/j.issn.1009-0959.2017.06.022
[14]朱晓燕,彭成,钟振东,等.香连金黄散外治阳证疮疡对皮肤炎症微环境影响的实验研究[J].时珍国医国药,2016,27(07):1540-1543.DOI:10.3969/j.issn.1008-0805.2016.07.002
[15]李凤蕾,杜菲,吴迪,等.乌灵菌粉早期应用对创伤后应激障碍模型大鼠行为及海马组织中IL-1β、IL-6水平的影响[J].中国药房,,2016,27(25):3478-3480.DOI:10.6039/j.issn.1001-0408.2016.25.07
[16]张莉,杜冠华.炎性细胞因子抑制剂高通量筛选方法的研究[J].中国药理学通报,2003,(11):1260-1263.DOI:10.3321/j.issn:1001-1978.2003.11.015