超声波耦合酶解强化多效模拟连续逆流提取黄芪多糖
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摘要
将超声波强化和酶解强化同时应用于黄芪多糖的多效模拟连续逆流提取过程,通过优化单元内静态提取过程达到对整个连续逆流提取过程优化。以多糖提取率为指标,对比提取过程中不同的超声波强化与酶解强化组合方式,并通过单元提取单因素试验与Box-Behnken中心组合响应面试验优化得到超声波耦合酶解提取黄芪多糖的最佳工艺条件为:固液比例1:11、提取时间50 min、超声功率45 W、提取温度50℃,实际验证黄芪多糖提取率达到7.75%,综合得率达到88.98%,同时响应面分析结果表明固液比例与超声功率、提取温度与超声功率均存在显著交互作用。对于超声波耦合酶辅助连续逆流提取黄芪多糖设备的设计制造与优化有一定的指导意义。
In this paper, the multi-effect simulative continuous countercurrent extraction(CCE) was assisted by ultrasound and enzymatic hydrolysis to extract Astragalus polysaccharide. The optimization of the CCE was achieved by the static extraction process simulating the unit. Taking the extraction rate of Astragalus polysaccharide(APS) as a response value, different combination methods of ultrasound and enzymatic hydrolysis in the extraction process were compared. The single-factor static extraction method and the Box-Behnken design center combined response surface test were used to optimize the extraction of APS assisted by ultrasound coupling enzymatic hydrolysis. The optimum process conditions were: solid-liquid ratio 1:11, extraction time 50 min, ultrasonic power 45 W, extraction temperature 50 ℃. The actual extraction rate of APS reached 7.75%, the comprehensive yield reached 88.98%. The results of response surface analysis showed that there was a significant interaction between solid-liquid ratio and ultrasonic power, extraction temperature and ultrasonic power. It showed certain guiding significance for the design, manufacture and optimization of ultrasonically and enzymatically assisted continuous countercurrent extraction of APS.
In this paper, the multi-effect simulative continuous countercurrent extraction(CCE) was assisted by ultrasound and enzymatic hydrolysis to extract Astragalus polysaccharide. The optimization of the CCE was achieved by the static extraction process simulating the unit. Taking the extraction rate of Astragalus polysaccharide(APS) as a response value, different combination methods of ultrasound and enzymatic hydrolysis in the extraction process were compared. The single-factor static extraction method and the Box-Behnken design center combined response surface test were used to optimize the extraction of APS assisted by ultrasound coupling enzymatic hydrolysis. The optimum process conditions were: solid-liquid ratio 1:11, extraction time 50 min, ultrasonic power 45 W, extraction temperature 50 ℃. The actual extraction rate of APS reached 7.75%, the comprehensive yield reached 88.98%. The results of response surface analysis showed that there was a significant interaction between solid-liquid ratio and ultrasonic power, extraction temperature and ultrasonic power. It showed certain guiding significance for the design, manufacture and optimization of ultrasonically and enzymatically assisted continuous countercurrent extraction of APS.
引文
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[12]梁霞,吕晓玲.复合酶和超声辅助两步法提取甘草酸的工艺[J].食品与机械,2018,34(10):145-151.
[13]陈艳,姚密,李美凤,等.超声波辅助复合酶法提取松茸多糖的工艺研究[J].中国酿造,2017,36(10):139-143.
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[18]WANG T,GUO N,WANG S,et al.Ultrasound-negative Pressure Cavitation Extraction of Phenolic Compounds from Blueberry Leaves and Evaluation of its DPPHRadical Scavenging Activity[J].Food and Bioproducts Processing,2018,108:69-80.
[19]RITTIRUT W,THONGURAI C,SIRIPATANA C.Mathematical Simulation of Solid-Liquid Diffusion in Continuous Countercurrrent Extraction Process,Part II-Modeling Verification and Its Application[J].International Journal of Chemical Reacter Engineering,2010,(8):A133.
[2]CAO Yuan,SHEN Tao,HUANG Xiuqing,et al.Astragalus Polysaccharide Restores Autophagic Flux and Improves Cardiomyocyte Function in Doxorubicin-induced Cardiotoxicity[J].Oncotarget,2017,8(3):4837-4848.
[3]WEI Wei,XIAO Haitao,BAO Wanrong,et al.TLR-4 May Mediate Signaling Pathways of Astragalus Polysaccharide RAP Induced Cytokine Expression of RAW264.7 Cells[J].Journal of Ethnopharmacology,2016,179(8):243-252.
[4]张晓,朱彩平,邓红,等.均匀设计优化超声协同酶法提取平菇多糖工艺[J].食品与机械,2016,32(9):166-171.
[5]张慧瑛,罗光宏,郝军元,等.超声波-生物酶法提取锁阳多糖工艺优化及其抗肿瘤活性[J].食品科学,2016,37(12):59-64.
[6]谢丽源,王富伟,李洪军,等.超声波复合酶法提取桑黄多糖研究[J].食品科学,2010,31(10):81-85.
[7]陈,田景奎.一种中药动态连续逆流提取的实验室模拟方法[J].亚太传统医药,2006,(1):60-64.
[8]张毅,宁正祥,董华强,等.多穗柯总黄酮的连续逆流提取研究[J].现代食品科技,2011,27(5):550-552.
[9]战宇,梁敏华.亮叶杨桐叶中类黄酮的连续逆流提取及抗氧化活性研究[J].食品科学,2010,31(14):97-100.
[10]栾连军,陈娜,刘雪松,等.葡萄籽原花青素多级逆流提取工艺研究[J].中国食品学报,2010,10(5):31-36.
[11]谢红旗,季可可,何英杰,等.模拟连续逆流提取黄柏中小檗碱工艺研究[J].天然产物研究与开发,2017,(11):1959-1965.
[12]梁霞,吕晓玲.复合酶和超声辅助两步法提取甘草酸的工艺[J].食品与机械,2018,34(10):145-151.
[13]陈艳,姚密,李美凤,等.超声波辅助复合酶法提取松茸多糖的工艺研究[J].中国酿造,2017,36(10):139-143.
[14]苏杭丽.超声波在悬浮液中的衰减[J].河海大学学报(自然科学版),2012,40(6):710-714.
[15]ROMDHANE M,GADRI A,CONTAMINE F,et al.Experimental Study of the Ultrasound Attenuation in Chemical Reactors[J].Ultrasonics Sonochemistry,1997,4(3):235-243.
[16]GUéDRA M,CORNU C,INSERRA C.A Derivation of the Stable Cavitation Threshold Accounting for BubbleBubble Interactions[J].Ultrasonics Sonochemistry,2017,38:168-173.
[17]REUTER F,LESNIK S,AYAZ-BUSTAMI K,et al.Bubble Size Measurements in Different Acoustic Cavitation Structures:Filaments,Clusters,and The Acoustically Cavitated Jet[J].Ultrasonics Sonochemistry,2018.
[18]WANG T,GUO N,WANG S,et al.Ultrasound-negative Pressure Cavitation Extraction of Phenolic Compounds from Blueberry Leaves and Evaluation of its DPPHRadical Scavenging Activity[J].Food and Bioproducts Processing,2018,108:69-80.
[19]RITTIRUT W,THONGURAI C,SIRIPATANA C.Mathematical Simulation of Solid-Liquid Diffusion in Continuous Countercurrrent Extraction Process,Part II-Modeling Verification and Its Application[J].International Journal of Chemical Reacter Engineering,2010,(8):A133.