一株Nitratireductor sp.WJ5-4的生理生化特性及产电分析
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摘要
主要研究了n itratireductor sp.WJ5-4的生化特性,并以Nitratireductor sp.WJ5-4为产电菌构建双室盐桥微生物燃料电池.研究微生物燃料电池在葡萄糖不同浓度及不同碳源条件下的降解和产电情况,并通过极化曲线和能量密度曲线表征电池的内阻和产能情况.实验结果表明:Nitratireductor sp.WJ5-4厌氧菌,耐氧性能较差.添加酵母膏作为生长因子能明显促进Nitratireductor sp.WJ5-4生长,最适生长温度为35℃.可利用多种碳源生长,其中利用葡萄糖、乳糖、柠檬酸三铵生长良好.当葡萄糖浓度为11g/L时,输出最大稳定工作电压290mV,具有最大的功率密度96.13mW/m2和较高的CODCr处理效率77.03%.不同碳源为底物时,利用葡萄糖产电,可获得最大功率密度103.84 mW/m2、最大稳定输出电压450mV和最大CODCr处理效率83.60%.
Physiological and biochemical characteristics of Nitratireductor sp. WJ5- 4 was studied and two- chamber microbial fuel cells( MFCs) was constructed using Nitratireductor sp. WJ5- 4 as electricigen bacteria. Degradation and electricity generation was studied through polarization curve and energy density curve under different concentration of glucose and different carbon source. The results showed when the glucose concentration was 11g / L,the maximum stable working voltage was 290mV, power density was 96. 13mW / m2and the removal of CODCrwas up to 77. 03%. when glucose was substrate,power density was 103. 84mW / m2,the maximum voltage was 450mV,and the removal of CODCrwas up to 83. 60%.
Physiological and biochemical characteristics of Nitratireductor sp. WJ5- 4 was studied and two- chamber microbial fuel cells( MFCs) was constructed using Nitratireductor sp. WJ5- 4 as electricigen bacteria. Degradation and electricity generation was studied through polarization curve and energy density curve under different concentration of glucose and different carbon source. The results showed when the glucose concentration was 11g / L,the maximum stable working voltage was 290mV, power density was 96. 13mW / m2and the removal of CODCrwas up to 77. 03%. when glucose was substrate,power density was 103. 84mW / m2,the maximum voltage was 450mV,and the removal of CODCrwas up to 83. 60%.
引文
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[3]Reimers C E,Tender L M,Fertig S,et al.Harvesting Energy from the Marine Sediment-water Interface[J].Environ Sci Technol,2001,35(1):192-195.
[4]Kim H J,Park H S,Hyun M S,et al.A Mediator-less Microbial Fuel Cell Using a Metal Reducing Bacterium,Shewanella Putrefaciens[J].Enzyme Microb Technol,2002,30(2):145-152.
[5]Gil G C,Chang I S,Kim B H,et al.Operational Parameters Affecting the Performance of a Mediator-less Microbial Fuel Cell[J].Biosens Bioelectron,2003,18(4):327-334.
[6]Logan B E,Hamelers B,Rozendal R,et al.Microbial Fuel Cells:Methodology and Technology[J].Environ Sci Technol,2006,40(17):5181-5192.
[7]Oh S E,Logan B E.Hydrogen and Electricity Production from a Food Processing Wastewater Using Fermentation and Microbial Fuel Cell Technologies[J].Water Res,2005,39(19):4673-4682.
[8]Min B,Kim J R,Oh S E,et al.Electricity Generation from Swine Wastewater Using Microbial Fuel Cells[J].Water Res,2005,39(20):4961-4968.
[9]Min B,Logan B E.Continuous Electricity Generation from Domestic Wastewatrand Organic Substrates in a Flat Plate Microbial Fuel Cell[J].Environ Sci Technol,2004,38(21):5809-5814.
[10]Zuo Y,Maness P C,Logan B E.Electricity Production from Steam-exploded Corn Stover Biomass[J].Energy Fuel,2006,20(4):1716-1721.
[11]Xing D F,Zuo Y,Cheng S,et al.Electricity Generation by Rhodopseudomonas Palustris DX-1[J].Environmental Science and Technology,2008,42:4146-4151.
[12]晨风.英国河流发现太空细菌有望成为发电新能源[EB/OL].(2012-02-24).[2013-03-25]http://news.xinhuanet.com/tech/2012-02/24/c_122748750.htm,
[13]黄霞,范明志,梁鹏,等.微生物燃料电池阳极特性对产电性能的影响[J].中国给水排水,2007,23(3):8-12.
[14]Holmes D E,Bond D R,Lovley D R.Electron transfer by Desulfobulbus Propionicusto Fe(Ⅲ)and Graphite Electrodes[J].Appl Environ Microbiol,2004,70(2):1234-1237.
[15]奚旦立.环境监测实验[M].北京:高等教育出版社,2011:35-37.
[16]Miller G L.Use of Dinitrosalicylic Acid Reagent for the Determination of Reducing Sugars[J].Anal Chem,1959,31(3):426-428.
[17]胡家骏,周群英.环境工程微生物学[M].北京:高等教育出版社,2000:115.
[18]张翼峰.不同底物的微生物燃料电池阳极菌群及其产电特性分析[D].大连:大连理工大学环境学院,2008.