miR-208作为急性心肌梗死的标志物研究
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摘要
目的研究miR-208在急性心肌梗死(AMI)患者中的表达水平。方法入选2015年4—12月住院和健康体检者共91例,其中包括明确诊断为AMI的患者53例(AMI组)和健康对照者38例(对照组)。收集急性心肌梗死后2小时内采血,分离血浆提取总RNA,用TaqMan~? microRNA RT-PCR试剂盒进行反转录,使用线虫microRNA cel-miR-39做内参,采用相对Cq法(ΔCq)检测血浆中miR-208表达水平。结果 AMI组血浆miR-208的中位值为1.24,对照组血浆miR-208的中位值为0.14,采用直线相关统计学分析AMI组血浆miR-208显著高于对照组(P=0.013)。ROC曲线下面积(AUC)为0.780,miR-208诊断AMI的敏感性和特异性分别为88%和68%。结论急性心肌梗死患者血浆miR-208在心梗发生后2小时内表达量显著升高,miR-208可能对于急性心肌梗死的诊断具有一定价值。
Objective To evaluate the plasma miR-208 expression level as a diagnostic biomarker for acute myocardial infarction(AMI). Methods91 patients were enrolled from April to December 2015, including 53 patients diagnosed with AMI(AMI group) and 38 healthy controls(control group). The patients' blood was collected after AMI within 2 hours. Total RNA was extracted from separated plasma, the TaqMan~? microRNA RT-PCR was used to reverse transcript cDNA. Nematodes microRNA cel-miR-39 as reference gene. The expression of miR-208 in plasma was measured by relative Cq method(ΔCq). Results The median value of plasma miR-208 in AMI group was 1.24. The median of plasma miR-208 in the control group was 0.14. Linear correlation analysis showed that the level of plasma miR-208 in AMI group was significantly higher than that in control group(P=0.013). The area under the ROC curve(AUC) was 0.780, and the sensitivity and specificity of MI-208 in the diagnosis of AMI were 88% and 68%, respectively. Conclusion The expression level of plasma miR-208 in AMI group increased significantly within 2 hours after myocardial infarction, which may serve as a potention biomarker for AMI.
Objective To evaluate the plasma miR-208 expression level as a diagnostic biomarker for acute myocardial infarction(AMI). Methods91 patients were enrolled from April to December 2015, including 53 patients diagnosed with AMI(AMI group) and 38 healthy controls(control group). The patients' blood was collected after AMI within 2 hours. Total RNA was extracted from separated plasma, the TaqMan~? microRNA RT-PCR was used to reverse transcript cDNA. Nematodes microRNA cel-miR-39 as reference gene. The expression of miR-208 in plasma was measured by relative Cq method(ΔCq). Results The median value of plasma miR-208 in AMI group was 1.24. The median of plasma miR-208 in the control group was 0.14. Linear correlation analysis showed that the level of plasma miR-208 in AMI group was significantly higher than that in control group(P=0.013). The area under the ROC curve(AUC) was 0.780, and the sensitivity and specificity of MI-208 in the diagnosis of AMI were 88% and 68%, respectively. Conclusion The expression level of plasma miR-208 in AMI group increased significantly within 2 hours after myocardial infarction, which may serve as a potention biomarker for AMI.
引文
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[13]Wang GK,Zhu JQ,Zhang JT,et al.Circulating microrna:a novel potential biomarker for early diagnosis of acute myocardial infarction in humans.European Heart Journal,2010,31(6):659-666.
[14]Sayed AS,Xia K,Yang TL,et al.Circulating micrornas:a potential role in diagnosis and prognosis of acute myocardial infarction[J].Disease Markers,2013,35(5):561-566.
[15]Devaux Y,Mueller M,Haaf P,et al.Diagnostic and prognostic value of circulating microRNAs in patients with acute chest pain[J].Journal of Internal Medicine,2015,277(2):260-271.
[2]钱军,李莹.高敏肌钙蛋白T在急性冠脉综合征早期诊断中的应用[J].国际心血管病杂志,2017,44(5):278-281.
[3]French JK,White HD.Clinical implications of the new definition of myocardial infarction[J].Heart 90(1):99-106.
[4]Li C,Fang Z,Jiang T,et al.Serum microRNAs profilefrom genome-wide serves as a fingerprint for diagnosis ofacute myocardial infarction and angina pectoris[J].BMC Medical Genomics,2013,6(1):16.
[5]Dangwal S,Thum T.microRNA therapeutics in cardiovascular disease models[J].Annu Rev Pharmacol Toxicol,2014,54(54):185-203.
[6]Liu X,Fan Z,Zhao T,et al.Plasma mir-1,mir-208,mir-499as potential predictive biomarkers for acute myocardial infarction:an independent study of han population[J].Experimental Gerontology,2015(72):230-238.
[7]Livak KJ,Schmittgen TD.Analysis of relative gene expression data using real-time quantitative pcr and the 2 deltact method[J].Methods,2001(4):402-408.
[8]Mitchell PS,Parkin RK,Kroh EM,et al.Circulating micrornas as stable blood-based markers for cancer detection[J].Proceedings of the National Academy of Sciences of the United States of America,2008,105(30):10513-10518.
[9]Callis TE,Pandya K,Seok HY,et al.Microrna-208a is a regulator of cardiac hypertrophy and conduction in mice[J].Journal of Clinical Investigation,2009,119(9):277-286.
[10]Montgomery RL,Hullinger TG,Semus HM,et al.Therapeutic inhibition of mir-208a improves cardiac function and survival during heart failure[J].Circulation,2011,124(14):1537-1547.
[11]Fichtlscherer S,De RS,Fox H,et al.Circulating micrornas in patients with coronary artery disease[J].Circulation Research,2010,107(5):677-684.
[12]Liu H,Yang N,Fei Z,et al.Analysis of plasma mir-208a and mir-370 expression levels for early diagnosis of coronary artery disease[J].Biomedical Reports,2016,5(3):332-336.
[13]Wang GK,Zhu JQ,Zhang JT,et al.Circulating microrna:a novel potential biomarker for early diagnosis of acute myocardial infarction in humans.European Heart Journal,2010,31(6):659-666.
[14]Sayed AS,Xia K,Yang TL,et al.Circulating micrornas:a potential role in diagnosis and prognosis of acute myocardial infarction[J].Disease Markers,2013,35(5):561-566.
[15]Devaux Y,Mueller M,Haaf P,et al.Diagnostic and prognostic value of circulating microRNAs in patients with acute chest pain[J].Journal of Internal Medicine,2015,277(2):260-271.