舟山群岛海洋沉积物中脂类生物标志物特征及物源解析
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摘要
为研究舟山海域表层沉积物的有机质来源和浮游植物群落结构,利用气相色谱-质谱联用(GCMS)和超高效液相色谱-质谱联用(UPLC-MS)分别对岱山、衡山和嵊泗3个岛屿附近的23个站点的表层海洋沉积物中的非极性脂和极性膜脂进行分析。结果显示舟山沉积物中舟山沉积物的有机质来源主要有陆源输入和海源输入,其中陆源输入为60%左右,海洋输入为40%左右。舟山沉积物中的陆源正构烷烃与海源正构烷烃之比(∑T/∑M)值在0.54~2.73之间,高∑T/∑M值区域靠近杭州湾,低∑T/∑M值区域靠近东海近岸海域。与长江口区域相比,舟山沉积物受到的陆源输入影响较小,但远远高于东海赤潮区域。沉积物中的磷脂酰甘油含量较高,脂酰乙醇胺和磷脂酰肌醇含量较低,脂肪酸参数∑_(C16)/∑C_(18)在1附近。这说明舟山沉积物中的主要浮游植物是微藻,但甲藻和硅藻无各自优势。
In order to study the origin of organic matter and phytoplankton community structure of marine sediments in Zhoushan,the non-polar matter and intact phospholipids composition in the sediment cores at 23 sites of the Daishan,Hengshan and Shengsi islands was examined by GC-MS and UPLC-MS. The study showed that the sediments organic material in the Zhoushan was derived from both terrestrial plants and marine organism,of which terrestrial inputs were about 60% and marine inputs were about 40%. The range of the ratio of high to low carbon hydrocarbon( ∑T/∑M)was between 0. 54 and 2. 73,and the high ∑T/∑M value area was close to Hangzhou bay,the low ∑T/∑M value area was near the coastal of the East China sea. Compared with the Yangtze estuary area,Zhoushan sediment was less affected by terrestrial input,but much higher than the tide-frequent-occurrence area in the East China Sea. The content of Phosphatidylglycerol in sediments was higher than the Phosphatidylethanolamine and Phosphatidylinositol,the fatty acid parameter ∑C_(16)/∑C_(18) was near 1. These results indicated that the main Phytoplankton in the sediments of Zhoushan was microalgae,and dinoflagellates and diatoms had no special advantages.
In order to study the origin of organic matter and phytoplankton community structure of marine sediments in Zhoushan,the non-polar matter and intact phospholipids composition in the sediment cores at 23 sites of the Daishan,Hengshan and Shengsi islands was examined by GC-MS and UPLC-MS. The study showed that the sediments organic material in the Zhoushan was derived from both terrestrial plants and marine organism,of which terrestrial inputs were about 60% and marine inputs were about 40%. The range of the ratio of high to low carbon hydrocarbon( ∑T/∑M)was between 0. 54 and 2. 73,and the high ∑T/∑M value area was close to Hangzhou bay,the low ∑T/∑M value area was near the coastal of the East China sea. Compared with the Yangtze estuary area,Zhoushan sediment was less affected by terrestrial input,but much higher than the tide-frequent-occurrence area in the East China Sea. The content of Phosphatidylglycerol in sediments was higher than the Phosphatidylethanolamine and Phosphatidylinositol,the fatty acid parameter ∑C_(16)/∑C_(18) was near 1. These results indicated that the main Phytoplankton in the sediments of Zhoushan was microalgae,and dinoflagellates and diatoms had no special advantages.
引文
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[18]李凤,刘亚娟,王江涛,等.东海赤潮高发区沉积物柱状样中正构烷烃和脂肪醇的分布与来源[J].沉积学报,2014,32(5):988-995.
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[2]王益鸣,王明翠,杨红丽,等.舟山群岛海洋动物体内石油烃的含量及对海域有机污染的指示作用[J].海洋环境科学,2008,27(6):610-614.
[3]GRIMALT J O,OLIVJ.Source input elucidation in aquatic systems by factor and principal component analysis of molecular marker data[J].Analytica Chimica Acta,1993,278(1):159-176.
[4]卢龙飞,蔡进功,雷天柱,等.东海表层沉积物黏土粒级中多态脂肪酸组成与地球化学特征[J].地球化学,2011,40(2):188-198.
[5]鲁晓红,陈颖军,黄国培,等.黄渤海表层沉积物中正构烷烃和甾醇的分布及来源研究[J].生态环境学报,2011,20(6/7):1117-1122.
[6]VOLKMAN J K,ROHJANS D,RULLKTTER J,et al.Sources and diagenesis of organic matter in tidal flat sediments from the German Wadden Sea[J].Continental Shelf Research,2000,20(10/11):1139-1158.
[7]BLIGH E G,DYER W J.A rapid method of total lipid extraction and purification[J].Canadian Journal of Biochemistry and Physiology,1959,37(8):911-917.
[8]WEHNER H,TESCHNER M,BOSECKER K.Chemical reactions and stability of biomarkers and stable isotope ratios during in vitro biodegradation of petroleum[J].Organic Geochemistry,1986,10(1):463-471.
[9]GEARING P,GEARING J N,LYTLE T F,et al.Hydrocarbons in60 northeast Gulf of Mexico shelf sediments:a preliminary survey[J].Geochimica et Cosmochimica Acta,1976,40(9):1005-1017.
[10]胡日军,吴建政,朱龙海,等.东海舟山群岛海域表层沉积物运移特性[J].中国海洋大学学报,2009,39(3):495-500,442.
[11]褚宏大,苏荣国,王修林,等.东海赤潮高发区表层沉积物中部分脂类标记物的分布与来源[J].海洋科学,2008,32(10):68-76.
[12]姜善春,贾蓉芬,王岩,等.中国南海北部湾海洋柱状沉积物中甾烯醇甾烷醇的演化[J].沉积学报,1991,9(S1):97-102.
[13]MEAD R,GOI M A.A lipid molecular marker assessment of sediments from the Northern Gulf of Mexico before and after the passage of Hurricane Lili[J].Organic Geochemistry,2006,37(9):1115-1129.
[14]HU J F,ZHANG H B,PENG P A.Fatty acid composition of surface sediments in the subtropical Pearl River estuary and adjacent shelf,southern China[J].Estuarine,Coastal and Shelf Science,2006,66(1/2):346-356.
[15]刘亚娟,王江涛,贺行良.东海赤潮高发区沉积物中脂肪酸分布及物源指示意义[J].海洋环境科学,2012,31(6):803-807.
[16]杨庶.典型海洋沉积物中脂类生物标志物的形态分布及来源分析[D].青岛:中国海洋大学,2009:10-12.
[17]郭志刚,杨作升,陈致林,等.东海陆架泥质区沉积有机质的物源分析[J].地球化学,2001,30(5):416-424.
[18]李凤,刘亚娟,王江涛,等.东海赤潮高发区沉积物柱状样中正构烷烃和脂肪醇的分布与来源[J].沉积学报,2014,32(5):988-995.
[19]RTTERS H,SASS H,CYPIONKA H,et al.Microbial communities in a Wadden Sea sediment core-clues from analyses of intact glyceride lipids,and released fatty acids[J].Organic Geochemistry,2002,33(7):803-816.