雄性C57BL/6J小鼠不同部位脂肪组织功能的差异
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摘要
目的 :研究雄性C57BL/6J小鼠各部位脂肪组织的棕色化功能差异。方法 :分离雄性C57BL/6J小鼠各部位脂肪组织,荧光定量PCR检测脂肪组织棕色化、成脂功能的标志基因、脂肪细胞因子、炎症因子表达情况。Western blot检测棕色化功能蛋白解偶联蛋白1(uncoupling protein-1,Ucp1)表达。结果:以肩胛间和肩胛下棕色脂肪为比较标准,其中甲状腺上、下部位脂肪的棕色化功能标志基因表达量最高,接近肩胛间和肩胛下;在肩胛间白色脂肪、腋下脂肪和肾周脂肪,上述棕色化标志基因表达量低于甲状腺周围脂肪;腹股沟皮下、附睾旁及肠系膜脂肪的上述棕色化标志基因表达最低。成脂功能的标志基因在皮下脂肪表达量明显高于附睾旁、肠系膜周围脂肪。脂肪细胞因子在白色脂肪特性的脂肪组织中表达量明显高于有棕色脂肪特性脂肪;附睾旁脂肪、肠系膜脂肪中炎症因子表达量显著升高,皮下脂肪炎症因子表达量最少。结论:甲状腺上、下部位脂肪的棕色化程度接近经典棕色脂肪。肩胛间白色脂肪、腋下脂肪和肾周脂肪较甲状腺周围脂肪的棕色化程度弱,成脂功能、脂肪细胞因子、炎症因子与肩胛间、肩胛下棕色脂肪基本一致且均较强。腹股沟皮下、附睾旁及肠系膜脂肪具有白色脂肪特性。皮下脂肪大量分泌脂肪细胞因子,炎症因子表达量小,成脂功能强。附睾旁脂肪、肠系膜周围脂肪细胞因子表达量较少,但炎症因子分泌相对较高且脂解作用较强。
Objective:To investigate browning ability differences of adipose tissues in each part of mice(male C57 BL / 6J).Methods: We isolated adipose tissues from mice(male C57 BL / 6J). The expressions of browning, adipogenesis, adipokine and inflammation markers were examined by fluorescence quantitative PCR. The protein expression of uncoupling protein-1(Ucp1) was detected by Western blotting. Results: The expression of browning genes in adipose tissues around the thyroid gland was the highest compared with those in the classic brown adipose tissues(interscapular and infrascapular brown adipose tissues). In mesenteric adipose tissues, perigonadal adipose tissues, and inguinal subcutaneous adipose tissues, browning genes showed the lowest expression. The expression of adipogenesis genes in subcutaneous adipose tissues was significantly higher than those in epididymis and mesenteric adipose tissues. The expression of adipocytokines in white adipocytes was significantly higher than that in brown adipocytes. The expression of inflammatory factor was significantly increased in perigonadal and mesenteric adipose tissues, while it was the lowest in subcutaneous adipose tissues. Conclusion: The browning ability in adipose tissues around the thyroid gland is close to that in interscapular and infrascapular brown adipose tissues. Adipose tissues around the thyroid gland, interscapular subcutaneous adipose tissues and adipose tissues from perirenal and axilla show brown-like abilities just like i BAT. Mesenteric adipose tissues,perigonadal adipose tissues, and inguinal subcutaneous adipose tissues showed strong abilities of adipogenesis and immune. The cytokine expression of mesenteric adipose tissues, perigonadal adipose tissues is lower, but inflammatory factor secretion is relatively higher, and lipolytic effect is stronger.
Objective:To investigate browning ability differences of adipose tissues in each part of mice(male C57 BL / 6J).Methods: We isolated adipose tissues from mice(male C57 BL / 6J). The expressions of browning, adipogenesis, adipokine and inflammation markers were examined by fluorescence quantitative PCR. The protein expression of uncoupling protein-1(Ucp1) was detected by Western blotting. Results: The expression of browning genes in adipose tissues around the thyroid gland was the highest compared with those in the classic brown adipose tissues(interscapular and infrascapular brown adipose tissues). In mesenteric adipose tissues, perigonadal adipose tissues, and inguinal subcutaneous adipose tissues, browning genes showed the lowest expression. The expression of adipogenesis genes in subcutaneous adipose tissues was significantly higher than those in epididymis and mesenteric adipose tissues. The expression of adipocytokines in white adipocytes was significantly higher than that in brown adipocytes. The expression of inflammatory factor was significantly increased in perigonadal and mesenteric adipose tissues, while it was the lowest in subcutaneous adipose tissues. Conclusion: The browning ability in adipose tissues around the thyroid gland is close to that in interscapular and infrascapular brown adipose tissues. Adipose tissues around the thyroid gland, interscapular subcutaneous adipose tissues and adipose tissues from perirenal and axilla show brown-like abilities just like i BAT. Mesenteric adipose tissues,perigonadal adipose tissues, and inguinal subcutaneous adipose tissues showed strong abilities of adipogenesis and immune. The cytokine expression of mesenteric adipose tissues, perigonadal adipose tissues is lower, but inflammatory factor secretion is relatively higher, and lipolytic effect is stronger.
引文
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[2]Sanchez-Gurmaches J,Guertin DA.Adipocyte lineages:Tracing back the origins of fat[J].Biochim Biophys Acta,2013,4(6):340-351
[3]Tchkonia T,Thomou T,Zhu Y,et al.Mechanisms and metabolic implications of regional differences among fat depots[J].Cell Metab,2013,17(5):644-656
[4]Cinti S.The adipose organ at a glance.[J].Dis Model Mech,2012,5(5):588-594
[5]Giralt M,Villarroya F.White,brown,beige/brite:different adipose cells for different functions?[J].Endocrinology,2013,154(9):2992-3000
[6]Cypess AM,Doyle AN,Sass CA,et al.Quantification of Human and rodent brown adipose tissue function using99m Tc-methoxyisobutylisonitrile SPECT/CT and 18FFDG PETCT[J].J Nuci Med,2013,54(11):1896-1901
[7]龚大为,李平,朱大龙.脂肪细胞因子研究进展[J].中国糖尿病杂志,2007,26(9):636-637
[8]Ross R,Aru J,Freeman J,et al.Abdominal adiposity and insulin resistance in obese men[J].Am J Physiol Endocrinal Metab,2002,282(3):567-663
[9]Vitali A,Murano I,Zingaretti MC,et al.The Adipose Organ of obesity-prone C57BL/6J mice is composed of mixed white and brown adipocytes[J].J Lipid Res,2012,53(4):619-629
[10]Wernstedt Asterholm I,Tao C,Morley TS,et al.Adipocyte inflammation is essential for healthy adipose tissue expansion and remodeling[J].Cell Metab,2014,20(1):103-118
[11]Suganami T,Ogawa Y.Adipose tissue macrophages:Their role in adipose tissue remodeling[J].J Leukoc Biol,2010,88(1):33-39
[12]Suganami T,Tanaka M,Ogawa Y.Adipose tissue inflammation and ectopic lipid accumulation[J].Endocr J,2012,25(7):849-857
[13]Panarotto D,Poisson J,Devroede G,et al.Lipoprotein lipase steadystate m RNA levels are lower in human omental versus subcutaneous abdominal adipose tissue[J].Metabolism,2000,49(9):1224-1227
[14]Ali AT,Hochfeld WE,Myburgh R,et al.Adipocyte and adipogenesis[J].Cell Biol,2014,1842(3):340-351
[15]Lee MJ,Wu Y,Fried SK.Adipose tissue heterogeneity:Implication of depot differences in adipose tissue for obesity complications[J].Mol Aspects Med,2013,34(1):1-11